Job ID = 14520618 SRX = SRX11781084 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:27:17 prefetch.2.10.7: 1) Downloading 'SRR15481027'... 2022-01-15T10:27:17 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:28:44 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:28:44 prefetch.2.10.7: 1) 'SRR15481027' was downloaded successfully 2022-01-15T10:28:44 prefetch.2.10.7: 'SRR15481027' has 0 unresolved dependencies Read 17997565 spots for SRR15481027/SRR15481027.sra Written 17997565 spots for SRR15481027/SRR15481027.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:09:24 17997565 reads; of these: 17997565 (100.00%) were paired; of these: 3393459 (18.86%) aligned concordantly 0 times 6690978 (37.18%) aligned concordantly exactly 1 time 7913128 (43.97%) aligned concordantly >1 times ---- 3393459 pairs aligned concordantly 0 times; of these: 96117 (2.83%) aligned discordantly 1 time ---- 3297342 pairs aligned 0 times concordantly or discordantly; of these: 6594684 mates make up the pairs; of these: 4465182 (67.71%) aligned 0 times 959914 (14.56%) aligned exactly 1 time 1169588 (17.74%) aligned >1 times 87.60% overall alignment rate Time searching: 01:09:24 Overall time: 01:09:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2249754 / 14698245 = 0.1531 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:56:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:56:18: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:56:18: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:56:23: 1000000 INFO @ Sat, 15 Jan 2022 20:56:29: 2000000 INFO @ Sat, 15 Jan 2022 20:56:34: 3000000 INFO @ Sat, 15 Jan 2022 20:56:39: 4000000 INFO @ Sat, 15 Jan 2022 20:56:44: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:56:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:56:48: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:56:48: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:56:50: 6000000 INFO @ Sat, 15 Jan 2022 20:56:54: 1000000 INFO @ Sat, 15 Jan 2022 20:56:55: 7000000 INFO @ Sat, 15 Jan 2022 20:56:59: 2000000 INFO @ Sat, 15 Jan 2022 20:57:01: 8000000 INFO @ Sat, 15 Jan 2022 20:57:04: 3000000 INFO @ Sat, 15 Jan 2022 20:57:06: 9000000 INFO @ Sat, 15 Jan 2022 20:57:10: 4000000 INFO @ Sat, 15 Jan 2022 20:57:11: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:57:16: 5000000 INFO @ Sat, 15 Jan 2022 20:57:17: 11000000 INFO @ Sat, 15 Jan 2022 20:57:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:57:18: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:57:18: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:57:22: 12000000 INFO @ Sat, 15 Jan 2022 20:57:23: 6000000 INFO @ Sat, 15 Jan 2022 20:57:25: 1000000 INFO @ Sat, 15 Jan 2022 20:57:27: 13000000 INFO @ Sat, 15 Jan 2022 20:57:29: 7000000 INFO @ Sat, 15 Jan 2022 20:57:31: 2000000 INFO @ Sat, 15 Jan 2022 20:57:33: 14000000 INFO @ Sat, 15 Jan 2022 20:57:35: 8000000 INFO @ Sat, 15 Jan 2022 20:57:37: 3000000 INFO @ Sat, 15 Jan 2022 20:57:38: 15000000 INFO @ Sat, 15 Jan 2022 20:57:41: 9000000 INFO @ Sat, 15 Jan 2022 20:57:43: 4000000 INFO @ Sat, 15 Jan 2022 20:57:43: 16000000 INFO @ Sat, 15 Jan 2022 20:57:47: 10000000 INFO @ Sat, 15 Jan 2022 20:57:49: 17000000 INFO @ Sat, 15 Jan 2022 20:57:49: 5000000 INFO @ Sat, 15 Jan 2022 20:57:53: 11000000 INFO @ Sat, 15 Jan 2022 20:57:54: 18000000 INFO @ Sat, 15 Jan 2022 20:57:55: 6000000 INFO @ Sat, 15 Jan 2022 20:57:59: 12000000 INFO @ Sat, 15 Jan 2022 20:57:59: 19000000 INFO @ Sat, 15 Jan 2022 20:58:01: 7000000 INFO @ Sat, 15 Jan 2022 20:58:05: 20000000 INFO @ Sat, 15 Jan 2022 20:58:05: 13000000 INFO @ Sat, 15 Jan 2022 20:58:07: 8000000 INFO @ Sat, 15 Jan 2022 20:58:10: 21000000 INFO @ Sat, 15 Jan 2022 20:58:11: 14000000 INFO @ Sat, 15 Jan 2022 20:58:13: 9000000 INFO @ Sat, 15 Jan 2022 20:58:15: 22000000 INFO @ Sat, 15 Jan 2022 20:58:18: 15000000 INFO @ Sat, 15 Jan 2022 20:58:19: 10000000 INFO @ Sat, 15 Jan 2022 20:58:21: 23000000 INFO @ Sat, 15 Jan 2022 20:58:24: 16000000 INFO @ Sat, 15 Jan 2022 20:58:25: 11000000 INFO @ Sat, 15 Jan 2022 20:58:26: 24000000 INFO @ Sat, 15 Jan 2022 20:58:30: 17000000 INFO @ Sat, 15 Jan 2022 20:58:31: 12000000 INFO @ Sat, 15 Jan 2022 20:58:32: 25000000 INFO @ Sat, 15 Jan 2022 20:58:35: 18000000 INFO @ Sat, 15 Jan 2022 20:58:37: 13000000 INFO @ Sat, 15 Jan 2022 20:58:38: 26000000 INFO @ Sat, 15 Jan 2022 20:58:42: 19000000 INFO @ Sat, 15 Jan 2022 20:58:43: 27000000 INFO @ Sat, 15 Jan 2022 20:58:44: 14000000 INFO @ Sat, 15 Jan 2022 20:58:44: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:58:44: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:58:44: #1 total tags in treatment: 12361976 INFO @ Sat, 15 Jan 2022 20:58:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:58:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:58:44: #1 tags after filtering in treatment: 4937699 INFO @ Sat, 15 Jan 2022 20:58:44: #1 Redundant rate of treatment: 0.60 INFO @ Sat, 15 Jan 2022 20:58:44: #1 finished! INFO @ Sat, 15 Jan 2022 20:58:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:58:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:58:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:58:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:58:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:58:48: 20000000 INFO @ Sat, 15 Jan 2022 20:58:50: 15000000 INFO @ Sat, 15 Jan 2022 20:58:54: 21000000 INFO @ Sat, 15 Jan 2022 20:58:56: 16000000 INFO @ Sat, 15 Jan 2022 20:59:00: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:59:02: 17000000 INFO @ Sat, 15 Jan 2022 20:59:06: 23000000 INFO @ Sat, 15 Jan 2022 20:59:09: 18000000 INFO @ Sat, 15 Jan 2022 20:59:13: 24000000 INFO @ Sat, 15 Jan 2022 20:59:15: 19000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:59:20: 25000000 INFO @ Sat, 15 Jan 2022 20:59:22: 20000000 INFO @ Sat, 15 Jan 2022 20:59:27: 26000000 INFO @ Sat, 15 Jan 2022 20:59:29: 21000000 INFO @ Sat, 15 Jan 2022 20:59:34: 27000000 INFO @ Sat, 15 Jan 2022 20:59:34: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:59:34: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:59:34: #1 total tags in treatment: 12361976 INFO @ Sat, 15 Jan 2022 20:59:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:59:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:59:34: #1 tags after filtering in treatment: 4937699 INFO @ Sat, 15 Jan 2022 20:59:34: #1 Redundant rate of treatment: 0.60 INFO @ Sat, 15 Jan 2022 20:59:34: #1 finished! INFO @ Sat, 15 Jan 2022 20:59:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:59:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:59:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:59:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:59:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:59:35: 22000000 INFO @ Sat, 15 Jan 2022 20:59:42: 23000000 INFO @ Sat, 15 Jan 2022 20:59:48: 24000000 INFO @ Sat, 15 Jan 2022 20:59:54: 25000000 INFO @ Sat, 15 Jan 2022 21:00:00: 26000000 INFO @ Sat, 15 Jan 2022 21:00:05: 27000000 INFO @ Sat, 15 Jan 2022 21:00:05: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 21:00:05: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 21:00:05: #1 total tags in treatment: 12361976 INFO @ Sat, 15 Jan 2022 21:00:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:00:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:00:06: #1 tags after filtering in treatment: 4937699 INFO @ Sat, 15 Jan 2022 21:00:06: #1 Redundant rate of treatment: 0.60 INFO @ Sat, 15 Jan 2022 21:00:06: #1 finished! INFO @ Sat, 15 Jan 2022 21:00:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:00:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:00:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:00:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:00:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781084/SRX11781084.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling