Job ID = 14520617 SRX = SRX11781083 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:27:17 prefetch.2.10.7: 1) Downloading 'SRR15481026'... 2022-01-15T10:27:17 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:29:01 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:29:01 prefetch.2.10.7: 1) 'SRR15481026' was downloaded successfully 2022-01-15T10:29:01 prefetch.2.10.7: 'SRR15481026' has 0 unresolved dependencies Read 21261280 spots for SRR15481026/SRR15481026.sra Written 21261280 spots for SRR15481026/SRR15481026.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:54 21261280 reads; of these: 21261280 (100.00%) were paired; of these: 3508163 (16.50%) aligned concordantly 0 times 15284363 (71.89%) aligned concordantly exactly 1 time 2468754 (11.61%) aligned concordantly >1 times ---- 3508163 pairs aligned concordantly 0 times; of these: 349832 (9.97%) aligned discordantly 1 time ---- 3158331 pairs aligned 0 times concordantly or discordantly; of these: 6316662 mates make up the pairs; of these: 3563369 (56.41%) aligned 0 times 2228220 (35.28%) aligned exactly 1 time 525073 (8.31%) aligned >1 times 91.62% overall alignment rate Time searching: 00:18:54 Overall time: 00:18:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3123015 / 18100666 = 0.1725 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:08:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:08:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:08:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:08:18: 1000000 INFO @ Sat, 15 Jan 2022 20:08:25: 2000000 INFO @ Sat, 15 Jan 2022 20:08:32: 3000000 INFO @ Sat, 15 Jan 2022 20:08:38: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:08:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:08:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:08:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:08:45: 5000000 INFO @ Sat, 15 Jan 2022 20:08:48: 1000000 INFO @ Sat, 15 Jan 2022 20:08:53: 6000000 INFO @ Sat, 15 Jan 2022 20:08:56: 2000000 INFO @ Sat, 15 Jan 2022 20:09:01: 7000000 INFO @ Sat, 15 Jan 2022 20:09:04: 3000000 INFO @ Sat, 15 Jan 2022 20:09:08: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:09:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:09:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:09:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:09:12: 4000000 INFO @ Sat, 15 Jan 2022 20:09:16: 9000000 INFO @ Sat, 15 Jan 2022 20:09:18: 1000000 INFO @ Sat, 15 Jan 2022 20:09:20: 5000000 INFO @ Sat, 15 Jan 2022 20:09:23: 10000000 INFO @ Sat, 15 Jan 2022 20:09:25: 2000000 INFO @ Sat, 15 Jan 2022 20:09:28: 6000000 INFO @ Sat, 15 Jan 2022 20:09:30: 11000000 INFO @ Sat, 15 Jan 2022 20:09:32: 3000000 INFO @ Sat, 15 Jan 2022 20:09:35: 7000000 INFO @ Sat, 15 Jan 2022 20:09:37: 12000000 INFO @ Sat, 15 Jan 2022 20:09:39: 4000000 INFO @ Sat, 15 Jan 2022 20:09:43: 8000000 INFO @ Sat, 15 Jan 2022 20:09:44: 13000000 INFO @ Sat, 15 Jan 2022 20:09:46: 5000000 INFO @ Sat, 15 Jan 2022 20:09:50: 9000000 INFO @ Sat, 15 Jan 2022 20:09:51: 14000000 INFO @ Sat, 15 Jan 2022 20:09:53: 6000000 INFO @ Sat, 15 Jan 2022 20:09:58: 10000000 INFO @ Sat, 15 Jan 2022 20:09:59: 15000000 INFO @ Sat, 15 Jan 2022 20:10:00: 7000000 INFO @ Sat, 15 Jan 2022 20:10:05: 11000000 INFO @ Sat, 15 Jan 2022 20:10:06: 16000000 INFO @ Sat, 15 Jan 2022 20:10:07: 8000000 INFO @ Sat, 15 Jan 2022 20:10:13: 17000000 INFO @ Sat, 15 Jan 2022 20:10:13: 12000000 INFO @ Sat, 15 Jan 2022 20:10:14: 9000000 INFO @ Sat, 15 Jan 2022 20:10:19: 18000000 INFO @ Sat, 15 Jan 2022 20:10:20: 13000000 INFO @ Sat, 15 Jan 2022 20:10:21: 10000000 INFO @ Sat, 15 Jan 2022 20:10:26: 19000000 INFO @ Sat, 15 Jan 2022 20:10:27: 11000000 INFO @ Sat, 15 Jan 2022 20:10:28: 14000000 INFO @ Sat, 15 Jan 2022 20:10:33: 20000000 INFO @ Sat, 15 Jan 2022 20:10:34: 12000000 INFO @ Sat, 15 Jan 2022 20:10:36: 15000000 INFO @ Sat, 15 Jan 2022 20:10:40: 21000000 INFO @ Sat, 15 Jan 2022 20:10:41: 13000000 INFO @ Sat, 15 Jan 2022 20:10:44: 16000000 INFO @ Sat, 15 Jan 2022 20:10:47: 22000000 INFO @ Sat, 15 Jan 2022 20:10:48: 14000000 INFO @ Sat, 15 Jan 2022 20:10:51: 17000000 INFO @ Sat, 15 Jan 2022 20:10:54: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:10:55: 15000000 INFO @ Sat, 15 Jan 2022 20:10:59: 18000000 INFO @ Sat, 15 Jan 2022 20:11:01: 24000000 INFO @ Sat, 15 Jan 2022 20:11:02: 16000000 INFO @ Sat, 15 Jan 2022 20:11:07: 19000000 INFO @ Sat, 15 Jan 2022 20:11:08: 25000000 INFO @ Sat, 15 Jan 2022 20:11:09: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:11:15: 20000000 INFO @ Sat, 15 Jan 2022 20:11:15: 26000000 INFO @ Sat, 15 Jan 2022 20:11:16: 18000000 INFO @ Sat, 15 Jan 2022 20:11:22: 21000000 INFO @ Sat, 15 Jan 2022 20:11:22: 27000000 INFO @ Sat, 15 Jan 2022 20:11:24: 19000000 INFO @ Sat, 15 Jan 2022 20:11:30: 28000000 INFO @ Sat, 15 Jan 2022 20:11:30: 22000000 INFO @ Sat, 15 Jan 2022 20:11:31: 20000000 INFO @ Sat, 15 Jan 2022 20:11:37: 29000000 INFO @ Sat, 15 Jan 2022 20:11:37: 23000000 INFO @ Sat, 15 Jan 2022 20:11:38: 21000000 INFO @ Sat, 15 Jan 2022 20:11:44: 30000000 INFO @ Sat, 15 Jan 2022 20:11:45: 24000000 INFO @ Sat, 15 Jan 2022 20:11:45: 22000000 INFO @ Sat, 15 Jan 2022 20:11:51: 31000000 INFO @ Sat, 15 Jan 2022 20:11:52: 23000000 INFO @ Sat, 15 Jan 2022 20:11:52: 25000000 INFO @ Sat, 15 Jan 2022 20:11:57: 32000000 INFO @ Sat, 15 Jan 2022 20:11:59: 24000000 INFO @ Sat, 15 Jan 2022 20:12:00: 26000000 INFO @ Sat, 15 Jan 2022 20:12:03: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:12:03: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:12:03: #1 total tags in treatment: 14660487 INFO @ Sat, 15 Jan 2022 20:12:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:12:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:12:03: #1 tags after filtering in treatment: 8500900 INFO @ Sat, 15 Jan 2022 20:12:03: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 20:12:03: #1 finished! INFO @ Sat, 15 Jan 2022 20:12:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:12:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:12:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:12:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:12:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:12:06: 25000000 INFO @ Sat, 15 Jan 2022 20:12:08: 27000000 INFO @ Sat, 15 Jan 2022 20:12:12: 26000000 INFO @ Sat, 15 Jan 2022 20:12:15: 28000000 INFO @ Sat, 15 Jan 2022 20:12:19: 27000000 INFO @ Sat, 15 Jan 2022 20:12:23: 29000000 INFO @ Sat, 15 Jan 2022 20:12:26: 28000000 INFO @ Sat, 15 Jan 2022 20:12:30: 30000000 INFO @ Sat, 15 Jan 2022 20:12:33: 29000000 INFO @ Sat, 15 Jan 2022 20:12:38: 31000000 INFO @ Sat, 15 Jan 2022 20:12:40: 30000000 INFO @ Sat, 15 Jan 2022 20:12:46: 32000000 INFO @ Sat, 15 Jan 2022 20:12:47: 31000000 INFO @ Sat, 15 Jan 2022 20:12:51: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:12:51: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:12:51: #1 total tags in treatment: 14660487 INFO @ Sat, 15 Jan 2022 20:12:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:12:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:12:51: #1 tags after filtering in treatment: 8500900 INFO @ Sat, 15 Jan 2022 20:12:51: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 20:12:51: #1 finished! INFO @ Sat, 15 Jan 2022 20:12:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:12:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:12:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:12:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:12:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 8 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:12:53: 32000000 INFO @ Sat, 15 Jan 2022 20:12:58: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:12:58: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:12:58: #1 total tags in treatment: 14660487 INFO @ Sat, 15 Jan 2022 20:12:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:12:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:12:58: #1 tags after filtering in treatment: 8500900 INFO @ Sat, 15 Jan 2022 20:12:58: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 20:12:58: #1 finished! INFO @ Sat, 15 Jan 2022 20:12:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:12:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:12:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:12:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:12:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781083/SRX11781083.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling