Job ID = 14520616 SRX = SRX11781082 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 20336417 spots for SRR15481025/SRR15481025.sra Written 20336417 spots for SRR15481025/SRR15481025.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:17 20336417 reads; of these: 20336417 (100.00%) were paired; of these: 2903480 (14.28%) aligned concordantly 0 times 12018438 (59.10%) aligned concordantly exactly 1 time 5414499 (26.62%) aligned concordantly >1 times ---- 2903480 pairs aligned concordantly 0 times; of these: 115201 (3.97%) aligned discordantly 1 time ---- 2788279 pairs aligned 0 times concordantly or discordantly; of these: 5576558 mates make up the pairs; of these: 3380811 (60.63%) aligned 0 times 1418000 (25.43%) aligned exactly 1 time 777747 (13.95%) aligned >1 times 91.69% overall alignment rate Time searching: 00:29:17 Overall time: 00:29:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2274655 / 17546392 = 0.1296 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:16:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:16:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:16:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:16:16: 1000000 INFO @ Sat, 15 Jan 2022 20:16:21: 2000000 INFO @ Sat, 15 Jan 2022 20:16:27: 3000000 INFO @ Sat, 15 Jan 2022 20:16:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:16:39: 5000000 INFO @ Sat, 15 Jan 2022 20:16:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:16:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:16:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:16:44: 6000000 INFO @ Sat, 15 Jan 2022 20:16:45: 1000000 INFO @ Sat, 15 Jan 2022 20:16:50: 2000000 INFO @ Sat, 15 Jan 2022 20:16:50: 7000000 INFO @ Sat, 15 Jan 2022 20:16:55: 3000000 INFO @ Sat, 15 Jan 2022 20:16:56: 8000000 INFO @ Sat, 15 Jan 2022 20:16:59: 4000000 INFO @ Sat, 15 Jan 2022 20:17:02: 9000000 INFO @ Sat, 15 Jan 2022 20:17:04: 5000000 INFO @ Sat, 15 Jan 2022 20:17:08: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:17:09: 6000000 INFO @ Sat, 15 Jan 2022 20:17:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:17:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:17:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:17:14: 11000000 INFO @ Sat, 15 Jan 2022 20:17:14: 7000000 INFO @ Sat, 15 Jan 2022 20:17:15: 1000000 INFO @ Sat, 15 Jan 2022 20:17:19: 8000000 INFO @ Sat, 15 Jan 2022 20:17:19: 12000000 INFO @ Sat, 15 Jan 2022 20:17:20: 2000000 INFO @ Sat, 15 Jan 2022 20:17:24: 9000000 INFO @ Sat, 15 Jan 2022 20:17:25: 3000000 INFO @ Sat, 15 Jan 2022 20:17:25: 13000000 INFO @ Sat, 15 Jan 2022 20:17:29: 10000000 INFO @ Sat, 15 Jan 2022 20:17:30: 4000000 INFO @ Sat, 15 Jan 2022 20:17:31: 14000000 INFO @ Sat, 15 Jan 2022 20:17:34: 11000000 INFO @ Sat, 15 Jan 2022 20:17:34: 5000000 INFO @ Sat, 15 Jan 2022 20:17:37: 15000000 INFO @ Sat, 15 Jan 2022 20:17:39: 12000000 INFO @ Sat, 15 Jan 2022 20:17:39: 6000000 INFO @ Sat, 15 Jan 2022 20:17:43: 16000000 INFO @ Sat, 15 Jan 2022 20:17:43: 13000000 INFO @ Sat, 15 Jan 2022 20:17:44: 7000000 INFO @ Sat, 15 Jan 2022 20:17:48: 14000000 INFO @ Sat, 15 Jan 2022 20:17:48: 17000000 INFO @ Sat, 15 Jan 2022 20:17:49: 8000000 INFO @ Sat, 15 Jan 2022 20:17:53: 15000000 INFO @ Sat, 15 Jan 2022 20:17:54: 9000000 INFO @ Sat, 15 Jan 2022 20:17:54: 18000000 INFO @ Sat, 15 Jan 2022 20:17:58: 16000000 INFO @ Sat, 15 Jan 2022 20:17:59: 10000000 INFO @ Sat, 15 Jan 2022 20:18:00: 19000000 INFO @ Sat, 15 Jan 2022 20:18:03: 17000000 INFO @ Sat, 15 Jan 2022 20:18:04: 11000000 INFO @ Sat, 15 Jan 2022 20:18:06: 20000000 INFO @ Sat, 15 Jan 2022 20:18:08: 18000000 INFO @ Sat, 15 Jan 2022 20:18:09: 12000000 INFO @ Sat, 15 Jan 2022 20:18:11: 21000000 INFO @ Sat, 15 Jan 2022 20:18:13: 19000000 INFO @ Sat, 15 Jan 2022 20:18:14: 13000000 INFO @ Sat, 15 Jan 2022 20:18:17: 22000000 INFO @ Sat, 15 Jan 2022 20:18:18: 20000000 INFO @ Sat, 15 Jan 2022 20:18:18: 14000000 INFO @ Sat, 15 Jan 2022 20:18:23: 23000000 INFO @ Sat, 15 Jan 2022 20:18:23: 21000000 INFO @ Sat, 15 Jan 2022 20:18:23: 15000000 INFO @ Sat, 15 Jan 2022 20:18:27: 22000000 INFO @ Sat, 15 Jan 2022 20:18:28: 16000000 INFO @ Sat, 15 Jan 2022 20:18:28: 24000000 INFO @ Sat, 15 Jan 2022 20:18:32: 23000000 INFO @ Sat, 15 Jan 2022 20:18:33: 17000000 INFO @ Sat, 15 Jan 2022 20:18:34: 25000000 INFO @ Sat, 15 Jan 2022 20:18:37: 24000000 INFO @ Sat, 15 Jan 2022 20:18:38: 18000000 INFO @ Sat, 15 Jan 2022 20:18:40: 26000000 INFO @ Sat, 15 Jan 2022 20:18:42: 25000000 INFO @ Sat, 15 Jan 2022 20:18:43: 19000000 INFO @ Sat, 15 Jan 2022 20:18:46: 27000000 INFO @ Sat, 15 Jan 2022 20:18:47: 26000000 INFO @ Sat, 15 Jan 2022 20:18:47: 20000000 INFO @ Sat, 15 Jan 2022 20:18:52: 27000000 INFO @ Sat, 15 Jan 2022 20:18:52: 28000000 INFO @ Sat, 15 Jan 2022 20:18:52: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:18:57: 28000000 INFO @ Sat, 15 Jan 2022 20:18:57: 22000000 INFO @ Sat, 15 Jan 2022 20:18:58: 29000000 INFO @ Sat, 15 Jan 2022 20:19:01: 23000000 INFO @ Sat, 15 Jan 2022 20:19:01: 29000000 INFO @ Sat, 15 Jan 2022 20:19:03: 30000000 INFO @ Sat, 15 Jan 2022 20:19:06: 24000000 INFO @ Sat, 15 Jan 2022 20:19:06: 30000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:19:09: 31000000 INFO @ Sat, 15 Jan 2022 20:19:11: 25000000 INFO @ Sat, 15 Jan 2022 20:19:12: 31000000 INFO @ Sat, 15 Jan 2022 20:19:15: 32000000 INFO @ Sat, 15 Jan 2022 20:19:16: 26000000 INFO @ Sat, 15 Jan 2022 20:19:17: 32000000 INFO @ Sat, 15 Jan 2022 20:19:19: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:19:19: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:19:19: #1 total tags in treatment: 15164436 INFO @ Sat, 15 Jan 2022 20:19:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:19:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:19:20: #1 tags after filtering in treatment: 8209379 INFO @ Sat, 15 Jan 2022 20:19:20: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 20:19:20: #1 finished! INFO @ Sat, 15 Jan 2022 20:19:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:19:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:19:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:19:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:19:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:19:21: 27000000 INFO @ Sat, 15 Jan 2022 20:19:21: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:19:21: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:19:21: #1 total tags in treatment: 15164436 INFO @ Sat, 15 Jan 2022 20:19:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:19:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:19:21: #1 tags after filtering in treatment: 8209379 INFO @ Sat, 15 Jan 2022 20:19:21: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 20:19:21: #1 finished! INFO @ Sat, 15 Jan 2022 20:19:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:19:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:19:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:19:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:19:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 15 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:19:26: 28000000 INFO @ Sat, 15 Jan 2022 20:19:31: 29000000 INFO @ Sat, 15 Jan 2022 20:19:35: 30000000 INFO @ Sat, 15 Jan 2022 20:19:40: 31000000 INFO @ Sat, 15 Jan 2022 20:19:45: 32000000 INFO @ Sat, 15 Jan 2022 20:19:49: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:19:49: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:19:49: #1 total tags in treatment: 15164436 INFO @ Sat, 15 Jan 2022 20:19:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:19:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:19:49: #1 tags after filtering in treatment: 8209379 INFO @ Sat, 15 Jan 2022 20:19:49: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 20:19:49: #1 finished! INFO @ Sat, 15 Jan 2022 20:19:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:19:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:19:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:19:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:19:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781082/SRX11781082.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling