Job ID = 14520614 SRX = SRX11781080 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:27:06 prefetch.2.10.7: 1) Downloading 'SRR15481023'... 2022-01-15T10:27:06 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:28:56 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:28:56 prefetch.2.10.7: 1) 'SRR15481023' was downloaded successfully 2022-01-15T10:28:56 prefetch.2.10.7: 'SRR15481023' has 0 unresolved dependencies Read 20755236 spots for SRR15481023/SRR15481023.sra Written 20755236 spots for SRR15481023/SRR15481023.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:31:29 20755236 reads; of these: 20755236 (100.00%) were paired; of these: 5297990 (25.53%) aligned concordantly 0 times 5965742 (28.74%) aligned concordantly exactly 1 time 9491504 (45.73%) aligned concordantly >1 times ---- 5297990 pairs aligned concordantly 0 times; of these: 56352 (1.06%) aligned discordantly 1 time ---- 5241638 pairs aligned 0 times concordantly or discordantly; of these: 10483276 mates make up the pairs; of these: 6039582 (57.61%) aligned 0 times 1679839 (16.02%) aligned exactly 1 time 2763855 (26.36%) aligned >1 times 85.45% overall alignment rate Time searching: 01:31:29 Overall time: 01:31:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2428469 / 15512122 = 0.1566 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:26:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:26:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:26:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:26:09: 1000000 INFO @ Sat, 15 Jan 2022 21:26:18: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:26:28: 3000000 INFO @ Sat, 15 Jan 2022 21:26:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:26:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:26:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:26:38: 4000000 INFO @ Sat, 15 Jan 2022 21:26:41: 1000000 INFO @ Sat, 15 Jan 2022 21:26:47: 5000000 INFO @ Sat, 15 Jan 2022 21:26:53: 2000000 INFO @ Sat, 15 Jan 2022 21:26:57: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:27:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:27:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:27:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:27:04: 3000000 INFO @ Sat, 15 Jan 2022 21:27:07: 7000000 INFO @ Sat, 15 Jan 2022 21:27:09: 1000000 INFO @ Sat, 15 Jan 2022 21:27:16: 4000000 INFO @ Sat, 15 Jan 2022 21:27:17: 8000000 INFO @ Sat, 15 Jan 2022 21:27:18: 2000000 INFO @ Sat, 15 Jan 2022 21:27:27: 5000000 INFO @ Sat, 15 Jan 2022 21:27:27: 3000000 INFO @ Sat, 15 Jan 2022 21:27:28: 9000000 INFO @ Sat, 15 Jan 2022 21:27:35: 4000000 INFO @ Sat, 15 Jan 2022 21:27:38: 6000000 INFO @ Sat, 15 Jan 2022 21:27:38: 10000000 INFO @ Sat, 15 Jan 2022 21:27:43: 5000000 INFO @ Sat, 15 Jan 2022 21:27:49: 11000000 INFO @ Sat, 15 Jan 2022 21:27:50: 7000000 INFO @ Sat, 15 Jan 2022 21:27:52: 6000000 INFO @ Sat, 15 Jan 2022 21:27:59: 12000000 INFO @ Sat, 15 Jan 2022 21:28:00: 7000000 INFO @ Sat, 15 Jan 2022 21:28:01: 8000000 INFO @ Sat, 15 Jan 2022 21:28:08: 13000000 INFO @ Sat, 15 Jan 2022 21:28:09: 8000000 INFO @ Sat, 15 Jan 2022 21:28:13: 9000000 INFO @ Sat, 15 Jan 2022 21:28:17: 9000000 INFO @ Sat, 15 Jan 2022 21:28:18: 14000000 INFO @ Sat, 15 Jan 2022 21:28:23: 10000000 INFO @ Sat, 15 Jan 2022 21:28:26: 10000000 INFO @ Sat, 15 Jan 2022 21:28:28: 15000000 INFO @ Sat, 15 Jan 2022 21:28:35: 11000000 INFO @ Sat, 15 Jan 2022 21:28:35: 11000000 INFO @ Sat, 15 Jan 2022 21:28:39: 16000000 INFO @ Sat, 15 Jan 2022 21:28:44: 12000000 INFO @ Sat, 15 Jan 2022 21:28:46: 12000000 INFO @ Sat, 15 Jan 2022 21:28:49: 17000000 INFO @ Sat, 15 Jan 2022 21:28:54: 13000000 INFO @ Sat, 15 Jan 2022 21:28:58: 13000000 INFO @ Sat, 15 Jan 2022 21:29:00: 18000000 INFO @ Sat, 15 Jan 2022 21:29:03: 14000000 INFO @ Sat, 15 Jan 2022 21:29:10: 14000000 INFO @ Sat, 15 Jan 2022 21:29:12: 19000000 INFO @ Sat, 15 Jan 2022 21:29:13: 15000000 INFO @ Sat, 15 Jan 2022 21:29:21: 15000000 INFO @ Sat, 15 Jan 2022 21:29:22: 16000000 INFO @ Sat, 15 Jan 2022 21:29:24: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:29:31: 17000000 INFO @ Sat, 15 Jan 2022 21:29:33: 16000000 INFO @ Sat, 15 Jan 2022 21:29:35: 21000000 INFO @ Sat, 15 Jan 2022 21:29:40: 18000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:29:45: 17000000 INFO @ Sat, 15 Jan 2022 21:29:46: 22000000 INFO @ Sat, 15 Jan 2022 21:29:50: 19000000 INFO @ Sat, 15 Jan 2022 21:29:58: 23000000 INFO @ Sat, 15 Jan 2022 21:29:58: 18000000 INFO @ Sat, 15 Jan 2022 21:29:59: 20000000 INFO @ Sat, 15 Jan 2022 21:30:08: 24000000 INFO @ Sat, 15 Jan 2022 21:30:09: 21000000 INFO @ Sat, 15 Jan 2022 21:30:10: 19000000 INFO @ Sat, 15 Jan 2022 21:30:18: 22000000 INFO @ Sat, 15 Jan 2022 21:30:19: 25000000 INFO @ Sat, 15 Jan 2022 21:30:22: 20000000 INFO @ Sat, 15 Jan 2022 21:30:27: 23000000 INFO @ Sat, 15 Jan 2022 21:30:29: 26000000 INFO @ Sat, 15 Jan 2022 21:30:34: 21000000 INFO @ Sat, 15 Jan 2022 21:30:37: 24000000 INFO @ Sat, 15 Jan 2022 21:30:39: 27000000 INFO @ Sat, 15 Jan 2022 21:30:46: 22000000 INFO @ Sat, 15 Jan 2022 21:30:47: 25000000 INFO @ Sat, 15 Jan 2022 21:30:50: 28000000 INFO @ Sat, 15 Jan 2022 21:30:55: 26000000 INFO @ Sat, 15 Jan 2022 21:30:58: 23000000 INFO @ Sat, 15 Jan 2022 21:31:00: 29000000 INFO @ Sat, 15 Jan 2022 21:31:04: 27000000 INFO @ Sat, 15 Jan 2022 21:31:09: 24000000 INFO @ Sat, 15 Jan 2022 21:31:11: 30000000 INFO @ Sat, 15 Jan 2022 21:31:13: 28000000 INFO @ Sat, 15 Jan 2022 21:31:17: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 21:31:17: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 21:31:17: #1 total tags in treatment: 13031189 INFO @ Sat, 15 Jan 2022 21:31:17: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:31:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:31:17: #1 tags after filtering in treatment: 4974634 INFO @ Sat, 15 Jan 2022 21:31:17: #1 Redundant rate of treatment: 0.62 INFO @ Sat, 15 Jan 2022 21:31:17: #1 finished! INFO @ Sat, 15 Jan 2022 21:31:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:31:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:31:18: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:31:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:31:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:31:21: 29000000 INFO @ Sat, 15 Jan 2022 21:31:21: 25000000 INFO @ Sat, 15 Jan 2022 21:31:29: 30000000 INFO @ Sat, 15 Jan 2022 21:31:33: 26000000 INFO @ Sat, 15 Jan 2022 21:31:35: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 21:31:35: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 21:31:35: #1 total tags in treatment: 13031189 INFO @ Sat, 15 Jan 2022 21:31:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:31:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:31:35: #1 tags after filtering in treatment: 4974634 INFO @ Sat, 15 Jan 2022 21:31:35: #1 Redundant rate of treatment: 0.62 INFO @ Sat, 15 Jan 2022 21:31:35: #1 finished! INFO @ Sat, 15 Jan 2022 21:31:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:31:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:31:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:31:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:31:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:31:45: 27000000 INFO @ Sat, 15 Jan 2022 21:31:56: 28000000 INFO @ Sat, 15 Jan 2022 21:32:07: 29000000 INFO @ Sat, 15 Jan 2022 21:32:18: 30000000 INFO @ Sat, 15 Jan 2022 21:32:24: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 21:32:24: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 21:32:24: #1 total tags in treatment: 13031189 INFO @ Sat, 15 Jan 2022 21:32:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:32:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:32:25: #1 tags after filtering in treatment: 4974634 INFO @ Sat, 15 Jan 2022 21:32:25: #1 Redundant rate of treatment: 0.62 INFO @ Sat, 15 Jan 2022 21:32:25: #1 finished! INFO @ Sat, 15 Jan 2022 21:32:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:32:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:32:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:32:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:32:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781080/SRX11781080.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling