Job ID = 14520601 SRX = SRX11781079 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:25:39 prefetch.2.10.7: 1) Downloading 'SRR15481022'... 2022-01-15T10:25:39 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:26:47 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:26:47 prefetch.2.10.7: 1) 'SRR15481022' was downloaded successfully 2022-01-15T10:26:47 prefetch.2.10.7: 'SRR15481022' has 0 unresolved dependencies Read 12857372 spots for SRR15481022/SRR15481022.sra Written 12857372 spots for SRR15481022/SRR15481022.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:15 12857372 reads; of these: 12857372 (100.00%) were paired; of these: 1731226 (13.46%) aligned concordantly 0 times 9689586 (75.36%) aligned concordantly exactly 1 time 1436560 (11.17%) aligned concordantly >1 times ---- 1731226 pairs aligned concordantly 0 times; of these: 137577 (7.95%) aligned discordantly 1 time ---- 1593649 pairs aligned 0 times concordantly or discordantly; of these: 3187298 mates make up the pairs; of these: 2104321 (66.02%) aligned 0 times 908416 (28.50%) aligned exactly 1 time 174561 (5.48%) aligned >1 times 91.82% overall alignment rate Time searching: 00:17:15 Overall time: 00:17:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1766608 / 11262848 = 0.1569 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:58:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:58:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:58:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:58:29: 1000000 INFO @ Sat, 15 Jan 2022 19:58:42: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:58:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:58:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:58:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:58:55: 3000000 INFO @ Sat, 15 Jan 2022 19:58:59: 1000000 INFO @ Sat, 15 Jan 2022 19:59:09: 4000000 INFO @ Sat, 15 Jan 2022 19:59:13: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:59:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:59:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:59:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:59:22: 5000000 INFO @ Sat, 15 Jan 2022 19:59:27: 1000000 INFO @ Sat, 15 Jan 2022 19:59:27: 3000000 INFO @ Sat, 15 Jan 2022 19:59:35: 6000000 INFO @ Sat, 15 Jan 2022 19:59:37: 2000000 INFO @ Sat, 15 Jan 2022 19:59:41: 4000000 INFO @ Sat, 15 Jan 2022 19:59:47: 3000000 INFO @ Sat, 15 Jan 2022 19:59:48: 7000000 INFO @ Sat, 15 Jan 2022 19:59:55: 5000000 INFO @ Sat, 15 Jan 2022 19:59:56: 4000000 INFO @ Sat, 15 Jan 2022 20:00:01: 8000000 INFO @ Sat, 15 Jan 2022 20:00:05: 5000000 INFO @ Sat, 15 Jan 2022 20:00:08: 6000000 INFO @ Sat, 15 Jan 2022 20:00:14: 6000000 INFO @ Sat, 15 Jan 2022 20:00:15: 9000000 INFO @ Sat, 15 Jan 2022 20:00:21: 7000000 INFO @ Sat, 15 Jan 2022 20:00:23: 7000000 INFO @ Sat, 15 Jan 2022 20:00:29: 10000000 INFO @ Sat, 15 Jan 2022 20:00:33: 8000000 INFO @ Sat, 15 Jan 2022 20:00:35: 8000000 INFO @ Sat, 15 Jan 2022 20:00:42: 9000000 INFO @ Sat, 15 Jan 2022 20:00:42: 11000000 INFO @ Sat, 15 Jan 2022 20:00:48: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:00:51: 10000000 INFO @ Sat, 15 Jan 2022 20:00:55: 12000000 INFO @ Sat, 15 Jan 2022 20:01:00: 11000000 INFO @ Sat, 15 Jan 2022 20:01:01: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:01:09: 13000000 INFO @ Sat, 15 Jan 2022 20:01:10: 12000000 INFO @ Sat, 15 Jan 2022 20:01:15: 11000000 INFO @ Sat, 15 Jan 2022 20:01:19: 13000000 INFO @ Sat, 15 Jan 2022 20:01:22: 14000000 INFO @ Sat, 15 Jan 2022 20:01:28: 14000000 INFO @ Sat, 15 Jan 2022 20:01:28: 12000000 INFO @ Sat, 15 Jan 2022 20:01:35: 15000000 INFO @ Sat, 15 Jan 2022 20:01:37: 15000000 INFO @ Sat, 15 Jan 2022 20:01:41: 13000000 INFO @ Sat, 15 Jan 2022 20:01:46: 16000000 INFO @ Sat, 15 Jan 2022 20:01:48: 16000000 INFO @ Sat, 15 Jan 2022 20:01:55: 14000000 INFO @ Sat, 15 Jan 2022 20:01:56: 17000000 INFO @ Sat, 15 Jan 2022 20:02:00: 17000000 INFO @ Sat, 15 Jan 2022 20:02:04: 18000000 INFO @ Sat, 15 Jan 2022 20:02:08: 15000000 INFO @ Sat, 15 Jan 2022 20:02:13: 19000000 INFO @ Sat, 15 Jan 2022 20:02:13: 18000000 INFO @ Sat, 15 Jan 2022 20:02:20: 16000000 INFO @ Sat, 15 Jan 2022 20:02:22: 20000000 INFO @ Sat, 15 Jan 2022 20:02:22: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:02:22: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:02:22: #1 total tags in treatment: 9369030 INFO @ Sat, 15 Jan 2022 20:02:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:02:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:02:22: #1 tags after filtering in treatment: 5540275 INFO @ Sat, 15 Jan 2022 20:02:22: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 20:02:22: #1 finished! INFO @ Sat, 15 Jan 2022 20:02:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:02:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:02:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:02:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:02:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:02:26: 19000000 INFO @ Sat, 15 Jan 2022 20:02:32: 17000000 INFO @ Sat, 15 Jan 2022 20:02:39: 20000000 INFO @ Sat, 15 Jan 2022 20:02:40: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:02:40: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:02:40: #1 total tags in treatment: 9369030 INFO @ Sat, 15 Jan 2022 20:02:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:02:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:02:40: #1 tags after filtering in treatment: 5540275 INFO @ Sat, 15 Jan 2022 20:02:40: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 20:02:40: #1 finished! INFO @ Sat, 15 Jan 2022 20:02:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:02:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:02:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:02:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:02:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 319 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:02:45: 18000000 INFO @ Sat, 15 Jan 2022 20:02:58: 19000000 INFO @ Sat, 15 Jan 2022 20:03:10: 20000000 INFO @ Sat, 15 Jan 2022 20:03:11: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:03:11: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:03:11: #1 total tags in treatment: 9369030 INFO @ Sat, 15 Jan 2022 20:03:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:03:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:03:11: #1 tags after filtering in treatment: 5540275 INFO @ Sat, 15 Jan 2022 20:03:11: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 20:03:11: #1 finished! INFO @ Sat, 15 Jan 2022 20:03:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:03:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:03:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:03:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:03:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781079/SRX11781079.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling