Job ID = 14520599 SRX = SRX11781077 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:25:19 prefetch.2.10.7: 1) Downloading 'SRR15481020'... 2022-01-15T10:25:19 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:26:21 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:26:21 prefetch.2.10.7: 1) 'SRR15481020' was downloaded successfully 2022-01-15T10:26:21 prefetch.2.10.7: 'SRR15481020' has 0 unresolved dependencies Read 15802414 spots for SRR15481020/SRR15481020.sra Written 15802414 spots for SRR15481020/SRR15481020.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:50 15802414 reads; of these: 15802414 (100.00%) were paired; of these: 2406005 (15.23%) aligned concordantly 0 times 12071371 (76.39%) aligned concordantly exactly 1 time 1325038 (8.39%) aligned concordantly >1 times ---- 2406005 pairs aligned concordantly 0 times; of these: 198485 (8.25%) aligned discordantly 1 time ---- 2207520 pairs aligned 0 times concordantly or discordantly; of these: 4415040 mates make up the pairs; of these: 2999385 (67.94%) aligned 0 times 1220312 (27.64%) aligned exactly 1 time 195343 (4.42%) aligned >1 times 90.51% overall alignment rate Time searching: 00:19:50 Overall time: 00:19:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3409751 / 13593670 = 0.2508 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:01:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:01:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:01:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:02:02: 1000000 INFO @ Sat, 15 Jan 2022 20:02:12: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:02:21: 3000000 INFO @ Sat, 15 Jan 2022 20:02:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:02:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:02:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:02:31: 4000000 INFO @ Sat, 15 Jan 2022 20:02:33: 1000000 INFO @ Sat, 15 Jan 2022 20:02:40: 5000000 INFO @ Sat, 15 Jan 2022 20:02:43: 2000000 INFO @ Sat, 15 Jan 2022 20:02:49: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:02:52: 3000000 INFO @ Sat, 15 Jan 2022 20:02:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:02:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:02:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:02:59: 7000000 INFO @ Sat, 15 Jan 2022 20:03:03: 4000000 INFO @ Sat, 15 Jan 2022 20:03:03: 1000000 INFO @ Sat, 15 Jan 2022 20:03:09: 8000000 INFO @ Sat, 15 Jan 2022 20:03:14: 5000000 INFO @ Sat, 15 Jan 2022 20:03:14: 2000000 INFO @ Sat, 15 Jan 2022 20:03:18: 9000000 INFO @ Sat, 15 Jan 2022 20:03:24: 6000000 INFO @ Sat, 15 Jan 2022 20:03:24: 3000000 INFO @ Sat, 15 Jan 2022 20:03:28: 10000000 INFO @ Sat, 15 Jan 2022 20:03:35: 7000000 INFO @ Sat, 15 Jan 2022 20:03:35: 4000000 INFO @ Sat, 15 Jan 2022 20:03:38: 11000000 INFO @ Sat, 15 Jan 2022 20:03:45: 8000000 INFO @ Sat, 15 Jan 2022 20:03:45: 5000000 INFO @ Sat, 15 Jan 2022 20:03:48: 12000000 INFO @ Sat, 15 Jan 2022 20:03:55: 9000000 INFO @ Sat, 15 Jan 2022 20:03:56: 6000000 INFO @ Sat, 15 Jan 2022 20:03:58: 13000000 INFO @ Sat, 15 Jan 2022 20:04:06: 10000000 INFO @ Sat, 15 Jan 2022 20:04:06: 7000000 INFO @ Sat, 15 Jan 2022 20:04:07: 14000000 INFO @ Sat, 15 Jan 2022 20:04:16: 11000000 INFO @ Sat, 15 Jan 2022 20:04:16: 8000000 INFO @ Sat, 15 Jan 2022 20:04:17: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:04:27: 12000000 INFO @ Sat, 15 Jan 2022 20:04:27: 9000000 INFO @ Sat, 15 Jan 2022 20:04:27: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:04:37: 13000000 INFO @ Sat, 15 Jan 2022 20:04:37: 10000000 INFO @ Sat, 15 Jan 2022 20:04:38: 17000000 INFO @ Sat, 15 Jan 2022 20:04:48: 14000000 INFO @ Sat, 15 Jan 2022 20:04:48: 11000000 INFO @ Sat, 15 Jan 2022 20:04:48: 18000000 INFO @ Sat, 15 Jan 2022 20:04:58: 15000000 INFO @ Sat, 15 Jan 2022 20:04:58: 19000000 INFO @ Sat, 15 Jan 2022 20:04:59: 12000000 INFO @ Sat, 15 Jan 2022 20:05:08: 20000000 INFO @ Sat, 15 Jan 2022 20:05:09: 16000000 INFO @ Sat, 15 Jan 2022 20:05:09: 13000000 INFO @ Sat, 15 Jan 2022 20:05:18: 21000000 INFO @ Sat, 15 Jan 2022 20:05:19: 17000000 INFO @ Sat, 15 Jan 2022 20:05:20: 14000000 INFO @ Sat, 15 Jan 2022 20:05:26: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:05:26: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:05:26: #1 total tags in treatment: 10012678 INFO @ Sat, 15 Jan 2022 20:05:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:05:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:05:26: #1 tags after filtering in treatment: 5605754 INFO @ Sat, 15 Jan 2022 20:05:26: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 20:05:26: #1 finished! INFO @ Sat, 15 Jan 2022 20:05:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:05:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:05:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:05:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:05:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 151 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:05:30: 18000000 INFO @ Sat, 15 Jan 2022 20:05:30: 15000000 INFO @ Sat, 15 Jan 2022 20:05:41: 19000000 INFO @ Sat, 15 Jan 2022 20:05:41: 16000000 INFO @ Sat, 15 Jan 2022 20:05:51: 20000000 INFO @ Sat, 15 Jan 2022 20:05:51: 17000000 INFO @ Sat, 15 Jan 2022 20:06:01: 21000000 INFO @ Sat, 15 Jan 2022 20:06:02: 18000000 INFO @ Sat, 15 Jan 2022 20:06:09: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:06:09: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:06:09: #1 total tags in treatment: 10012678 INFO @ Sat, 15 Jan 2022 20:06:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:06:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:06:09: #1 tags after filtering in treatment: 5605754 INFO @ Sat, 15 Jan 2022 20:06:09: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 20:06:09: #1 finished! INFO @ Sat, 15 Jan 2022 20:06:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:06:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:06:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:06:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:06:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:06:12: 19000000 INFO @ Sat, 15 Jan 2022 20:06:22: 20000000 INFO @ Sat, 15 Jan 2022 20:06:32: 21000000 INFO @ Sat, 15 Jan 2022 20:06:40: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:06:40: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:06:40: #1 total tags in treatment: 10012678 INFO @ Sat, 15 Jan 2022 20:06:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:06:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:06:40: #1 tags after filtering in treatment: 5605754 INFO @ Sat, 15 Jan 2022 20:06:40: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 20:06:40: #1 finished! INFO @ Sat, 15 Jan 2022 20:06:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:06:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:06:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:06:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:06:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781077/SRX11781077.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling