Job ID = 14520572 SRX = SRX11781067 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:22:32 prefetch.2.10.7: 1) Downloading 'SRR15481010'... 2022-01-15T10:22:32 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:23:26 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:23:26 prefetch.2.10.7: 1) 'SRR15481010' was downloaded successfully 2022-01-15T10:23:26 prefetch.2.10.7: 'SRR15481010' has 0 unresolved dependencies Read 12525375 spots for SRR15481010/SRR15481010.sra Written 12525375 spots for SRR15481010/SRR15481010.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:10 12525375 reads; of these: 12525375 (100.00%) were paired; of these: 1578088 (12.60%) aligned concordantly 0 times 9579197 (76.48%) aligned concordantly exactly 1 time 1368090 (10.92%) aligned concordantly >1 times ---- 1578088 pairs aligned concordantly 0 times; of these: 93709 (5.94%) aligned discordantly 1 time ---- 1484379 pairs aligned 0 times concordantly or discordantly; of these: 2968758 mates make up the pairs; of these: 1949496 (65.67%) aligned 0 times 863977 (29.10%) aligned exactly 1 time 155285 (5.23%) aligned >1 times 92.22% overall alignment rate Time searching: 00:17:10 Overall time: 00:17:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1787656 / 11040070 = 0.1619 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:56:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:56:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:56:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:56:16: 1000000 INFO @ Sat, 15 Jan 2022 19:56:28: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:56:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:56:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:56:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:56:40: 3000000 INFO @ Sat, 15 Jan 2022 19:56:44: 1000000 INFO @ Sat, 15 Jan 2022 19:56:52: 4000000 INFO @ Sat, 15 Jan 2022 19:56:53: 2000000 INFO @ Sat, 15 Jan 2022 19:57:01: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:57:03: 5000000 INFO @ Sat, 15 Jan 2022 19:57:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:57:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:57:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:57:10: 4000000 INFO @ Sat, 15 Jan 2022 19:57:15: 6000000 INFO @ Sat, 15 Jan 2022 19:57:17: 1000000 INFO @ Sat, 15 Jan 2022 19:57:19: 5000000 INFO @ Sat, 15 Jan 2022 19:57:27: 7000000 INFO @ Sat, 15 Jan 2022 19:57:28: 6000000 INFO @ Sat, 15 Jan 2022 19:57:29: 2000000 INFO @ Sat, 15 Jan 2022 19:57:36: 7000000 INFO @ Sat, 15 Jan 2022 19:57:40: 8000000 INFO @ Sat, 15 Jan 2022 19:57:41: 3000000 INFO @ Sat, 15 Jan 2022 19:57:46: 8000000 INFO @ Sat, 15 Jan 2022 19:57:52: 9000000 INFO @ Sat, 15 Jan 2022 19:57:54: 4000000 INFO @ Sat, 15 Jan 2022 19:57:55: 9000000 INFO @ Sat, 15 Jan 2022 19:58:04: 10000000 INFO @ Sat, 15 Jan 2022 19:58:05: 10000000 INFO @ Sat, 15 Jan 2022 19:58:06: 5000000 INFO @ Sat, 15 Jan 2022 19:58:14: 11000000 INFO @ Sat, 15 Jan 2022 19:58:17: 11000000 INFO @ Sat, 15 Jan 2022 19:58:18: 6000000 INFO @ Sat, 15 Jan 2022 19:58:23: 12000000 INFO @ Sat, 15 Jan 2022 19:58:30: 7000000 INFO @ Sat, 15 Jan 2022 19:58:30: 12000000 INFO @ Sat, 15 Jan 2022 19:58:32: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:58:41: 14000000 INFO @ Sat, 15 Jan 2022 19:58:42: 8000000 INFO @ Sat, 15 Jan 2022 19:58:42: 13000000 INFO @ Sat, 15 Jan 2022 19:58:51: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:58:54: 9000000 INFO @ Sat, 15 Jan 2022 19:58:55: 14000000 INFO @ Sat, 15 Jan 2022 19:59:00: 16000000 INFO @ Sat, 15 Jan 2022 19:59:07: 10000000 INFO @ Sat, 15 Jan 2022 19:59:08: 15000000 INFO @ Sat, 15 Jan 2022 19:59:10: 17000000 INFO @ Sat, 15 Jan 2022 19:59:20: 11000000 INFO @ Sat, 15 Jan 2022 19:59:21: 16000000 INFO @ Sat, 15 Jan 2022 19:59:21: 18000000 INFO @ Sat, 15 Jan 2022 19:59:32: 19000000 INFO @ Sat, 15 Jan 2022 19:59:33: 12000000 INFO @ Sat, 15 Jan 2022 19:59:34: 17000000 INFO @ Sat, 15 Jan 2022 19:59:37: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 19:59:37: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 19:59:37: #1 total tags in treatment: 9166139 INFO @ Sat, 15 Jan 2022 19:59:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:59:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:59:37: #1 tags after filtering in treatment: 5546319 INFO @ Sat, 15 Jan 2022 19:59:37: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:59:37: #1 finished! INFO @ Sat, 15 Jan 2022 19:59:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:59:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:59:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:59:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:59:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:59:46: 13000000 INFO @ Sat, 15 Jan 2022 19:59:48: 18000000 INFO @ Sat, 15 Jan 2022 19:59:58: 14000000 INFO @ Sat, 15 Jan 2022 20:00:00: 19000000 INFO @ Sat, 15 Jan 2022 20:00:07: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:00:07: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:00:07: #1 total tags in treatment: 9166139 INFO @ Sat, 15 Jan 2022 20:00:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:00:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:00:07: #1 tags after filtering in treatment: 5546319 INFO @ Sat, 15 Jan 2022 20:00:07: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 20:00:07: #1 finished! INFO @ Sat, 15 Jan 2022 20:00:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:00:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:00:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:00:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:00:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:00:10: 15000000 INFO @ Sat, 15 Jan 2022 20:00:22: 16000000 INFO @ Sat, 15 Jan 2022 20:00:34: 17000000 INFO @ Sat, 15 Jan 2022 20:00:46: 18000000 INFO @ Sat, 15 Jan 2022 20:00:59: 19000000 INFO @ Sat, 15 Jan 2022 20:01:05: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 20:01:05: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 20:01:05: #1 total tags in treatment: 9166139 INFO @ Sat, 15 Jan 2022 20:01:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:01:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:01:05: #1 tags after filtering in treatment: 5546319 INFO @ Sat, 15 Jan 2022 20:01:05: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 20:01:05: #1 finished! INFO @ Sat, 15 Jan 2022 20:01:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:01:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:01:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:01:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:01:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 18 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781067/SRX11781067.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling