Job ID = 14520568 SRX = SRX11781063 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:22:32 prefetch.2.10.7: 1) Downloading 'SRR15481006'... 2022-01-15T10:22:32 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:23:20 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:23:20 prefetch.2.10.7: 1) 'SRR15481006' was downloaded successfully 2022-01-15T10:23:20 prefetch.2.10.7: 'SRR15481006' has 0 unresolved dependencies Read 10413132 spots for SRR15481006/SRR15481006.sra Written 10413132 spots for SRR15481006/SRR15481006.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:10 10413132 reads; of these: 10413132 (100.00%) were paired; of these: 1186013 (11.39%) aligned concordantly 0 times 7231258 (69.44%) aligned concordantly exactly 1 time 1995861 (19.17%) aligned concordantly >1 times ---- 1186013 pairs aligned concordantly 0 times; of these: 310268 (26.16%) aligned discordantly 1 time ---- 875745 pairs aligned 0 times concordantly or discordantly; of these: 1751490 mates make up the pairs; of these: 1232813 (70.39%) aligned 0 times 249201 (14.23%) aligned exactly 1 time 269476 (15.39%) aligned >1 times 94.08% overall alignment rate Time searching: 00:09:11 Overall time: 00:09:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 734696 / 9488513 = 0.0774 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:47:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:47:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:47:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:47:51: 1000000 INFO @ Sat, 15 Jan 2022 19:47:58: 2000000 INFO @ Sat, 15 Jan 2022 19:48:05: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:48:13: 4000000 INFO @ Sat, 15 Jan 2022 19:48:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:48:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:48:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:48:21: 5000000 INFO @ Sat, 15 Jan 2022 19:48:21: 1000000 INFO @ Sat, 15 Jan 2022 19:48:29: 6000000 INFO @ Sat, 15 Jan 2022 19:48:29: 2000000 INFO @ Sat, 15 Jan 2022 19:48:37: 7000000 INFO @ Sat, 15 Jan 2022 19:48:38: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:48:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:48:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:48:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:48:46: 8000000 INFO @ Sat, 15 Jan 2022 19:48:46: 4000000 INFO @ Sat, 15 Jan 2022 19:48:51: 1000000 INFO @ Sat, 15 Jan 2022 19:48:54: 9000000 INFO @ Sat, 15 Jan 2022 19:48:55: 5000000 INFO @ Sat, 15 Jan 2022 19:48:59: 2000000 INFO @ Sat, 15 Jan 2022 19:49:03: 6000000 INFO @ Sat, 15 Jan 2022 19:49:03: 10000000 INFO @ Sat, 15 Jan 2022 19:49:07: 3000000 INFO @ Sat, 15 Jan 2022 19:49:11: 7000000 INFO @ Sat, 15 Jan 2022 19:49:12: 11000000 INFO @ Sat, 15 Jan 2022 19:49:15: 4000000 INFO @ Sat, 15 Jan 2022 19:49:20: 8000000 INFO @ Sat, 15 Jan 2022 19:49:21: 12000000 INFO @ Sat, 15 Jan 2022 19:49:24: 5000000 INFO @ Sat, 15 Jan 2022 19:49:28: 9000000 INFO @ Sat, 15 Jan 2022 19:49:29: 13000000 INFO @ Sat, 15 Jan 2022 19:49:32: 6000000 INFO @ Sat, 15 Jan 2022 19:49:37: 10000000 INFO @ Sat, 15 Jan 2022 19:49:38: 14000000 INFO @ Sat, 15 Jan 2022 19:49:40: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:49:45: 11000000 INFO @ Sat, 15 Jan 2022 19:49:46: 15000000 INFO @ Sat, 15 Jan 2022 19:49:49: 8000000 INFO @ Sat, 15 Jan 2022 19:49:54: 12000000 INFO @ Sat, 15 Jan 2022 19:49:55: 16000000 INFO @ Sat, 15 Jan 2022 19:49:58: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:50:02: 13000000 INFO @ Sat, 15 Jan 2022 19:50:03: 17000000 INFO @ Sat, 15 Jan 2022 19:50:07: 10000000 INFO @ Sat, 15 Jan 2022 19:50:10: 14000000 INFO @ Sat, 15 Jan 2022 19:50:12: 18000000 INFO @ Sat, 15 Jan 2022 19:50:13: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:50:13: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:50:13: #1 total tags in treatment: 8508192 INFO @ Sat, 15 Jan 2022 19:50:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:50:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:50:13: #1 tags after filtering in treatment: 5948198 INFO @ Sat, 15 Jan 2022 19:50:13: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 19:50:13: #1 finished! INFO @ Sat, 15 Jan 2022 19:50:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:50:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:50:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:50:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:50:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:50:16: 11000000 INFO @ Sat, 15 Jan 2022 19:50:19: 15000000 INFO @ Sat, 15 Jan 2022 19:50:24: 12000000 INFO @ Sat, 15 Jan 2022 19:50:27: 16000000 INFO @ Sat, 15 Jan 2022 19:50:33: 13000000 INFO @ Sat, 15 Jan 2022 19:50:35: 17000000 INFO @ Sat, 15 Jan 2022 19:50:41: 14000000 INFO @ Sat, 15 Jan 2022 19:50:44: 18000000 INFO @ Sat, 15 Jan 2022 19:50:44: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:50:44: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:50:44: #1 total tags in treatment: 8508192 INFO @ Sat, 15 Jan 2022 19:50:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:50:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:50:45: #1 tags after filtering in treatment: 5948198 INFO @ Sat, 15 Jan 2022 19:50:45: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 19:50:45: #1 finished! INFO @ Sat, 15 Jan 2022 19:50:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:50:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:50:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:50:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:50:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:50:49: 15000000 INFO @ Sat, 15 Jan 2022 19:50:56: 16000000 INFO @ Sat, 15 Jan 2022 19:51:04: 17000000 INFO @ Sat, 15 Jan 2022 19:51:11: 18000000 INFO @ Sat, 15 Jan 2022 19:51:12: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:51:12: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:51:12: #1 total tags in treatment: 8508192 INFO @ Sat, 15 Jan 2022 19:51:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:12: #1 tags after filtering in treatment: 5948198 INFO @ Sat, 15 Jan 2022 19:51:12: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 19:51:12: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:51:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781063/SRX11781063.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling