Job ID = 14520520 SRX = SRX11781059 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:16:53 prefetch.2.10.7: 1) Downloading 'SRR15481002'... 2022-01-15T10:16:53 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:17:47 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:17:47 prefetch.2.10.7: 1) 'SRR15481002' was downloaded successfully 2022-01-15T10:17:47 prefetch.2.10.7: 'SRR15481002' has 0 unresolved dependencies Read 13862548 spots for SRR15481002/SRR15481002.sra Written 13862548 spots for SRR15481002/SRR15481002.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:52 13862548 reads; of these: 13862548 (100.00%) were paired; of these: 1326011 (9.57%) aligned concordantly 0 times 10027457 (72.33%) aligned concordantly exactly 1 time 2509080 (18.10%) aligned concordantly >1 times ---- 1326011 pairs aligned concordantly 0 times; of these: 398559 (30.06%) aligned discordantly 1 time ---- 927452 pairs aligned 0 times concordantly or discordantly; of these: 1854904 mates make up the pairs; of these: 1265774 (68.24%) aligned 0 times 289518 (15.61%) aligned exactly 1 time 299612 (16.15%) aligned >1 times 95.43% overall alignment rate Time searching: 00:11:52 Overall time: 00:11:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 985561 / 12877691 = 0.0765 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:44:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:44:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:44:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:44:35: 1000000 INFO @ Sat, 15 Jan 2022 19:44:40: 2000000 INFO @ Sat, 15 Jan 2022 19:44:45: 3000000 INFO @ Sat, 15 Jan 2022 19:44:51: 4000000 INFO @ Sat, 15 Jan 2022 19:44:56: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:44:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:44:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:44:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:45:02: 6000000 INFO @ Sat, 15 Jan 2022 19:45:05: 1000000 INFO @ Sat, 15 Jan 2022 19:45:07: 7000000 INFO @ Sat, 15 Jan 2022 19:45:10: 2000000 INFO @ Sat, 15 Jan 2022 19:45:13: 8000000 INFO @ Sat, 15 Jan 2022 19:45:16: 3000000 INFO @ Sat, 15 Jan 2022 19:45:18: 9000000 INFO @ Sat, 15 Jan 2022 19:45:21: 4000000 INFO @ Sat, 15 Jan 2022 19:45:23: 10000000 INFO @ Sat, 15 Jan 2022 19:45:27: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:45:29: 11000000 INFO @ Sat, 15 Jan 2022 19:45:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:45:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:45:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:45:32: 6000000 INFO @ Sat, 15 Jan 2022 19:45:34: 1000000 INFO @ Sat, 15 Jan 2022 19:45:34: 12000000 INFO @ Sat, 15 Jan 2022 19:45:38: 7000000 INFO @ Sat, 15 Jan 2022 19:45:39: 2000000 INFO @ Sat, 15 Jan 2022 19:45:40: 13000000 INFO @ Sat, 15 Jan 2022 19:45:43: 8000000 INFO @ Sat, 15 Jan 2022 19:45:44: 3000000 INFO @ Sat, 15 Jan 2022 19:45:46: 14000000 INFO @ Sat, 15 Jan 2022 19:45:49: 4000000 INFO @ Sat, 15 Jan 2022 19:45:49: 9000000 INFO @ Sat, 15 Jan 2022 19:45:51: 15000000 INFO @ Sat, 15 Jan 2022 19:45:54: 5000000 INFO @ Sat, 15 Jan 2022 19:45:55: 10000000 INFO @ Sat, 15 Jan 2022 19:45:57: 16000000 INFO @ Sat, 15 Jan 2022 19:45:58: 6000000 INFO @ Sat, 15 Jan 2022 19:46:01: 11000000 INFO @ Sat, 15 Jan 2022 19:46:02: 17000000 INFO @ Sat, 15 Jan 2022 19:46:03: 7000000 INFO @ Sat, 15 Jan 2022 19:46:06: 12000000 INFO @ Sat, 15 Jan 2022 19:46:08: 18000000 INFO @ Sat, 15 Jan 2022 19:46:08: 8000000 INFO @ Sat, 15 Jan 2022 19:46:12: 13000000 INFO @ Sat, 15 Jan 2022 19:46:13: 9000000 INFO @ Sat, 15 Jan 2022 19:46:13: 19000000 INFO @ Sat, 15 Jan 2022 19:46:17: 10000000 INFO @ Sat, 15 Jan 2022 19:46:18: 14000000 INFO @ Sat, 15 Jan 2022 19:46:19: 20000000 INFO @ Sat, 15 Jan 2022 19:46:22: 11000000 INFO @ Sat, 15 Jan 2022 19:46:24: 15000000 INFO @ Sat, 15 Jan 2022 19:46:24: 21000000 INFO @ Sat, 15 Jan 2022 19:46:27: 12000000 INFO @ Sat, 15 Jan 2022 19:46:29: 16000000 INFO @ Sat, 15 Jan 2022 19:46:30: 22000000 INFO @ Sat, 15 Jan 2022 19:46:32: 13000000 INFO @ Sat, 15 Jan 2022 19:46:35: 17000000 INFO @ Sat, 15 Jan 2022 19:46:35: 23000000 INFO @ Sat, 15 Jan 2022 19:46:37: 14000000 INFO @ Sat, 15 Jan 2022 19:46:41: 18000000 INFO @ Sat, 15 Jan 2022 19:46:41: 24000000 INFO @ Sat, 15 Jan 2022 19:46:42: 15000000 INFO @ Sat, 15 Jan 2022 19:46:44: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:46:44: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:46:44: #1 total tags in treatment: 11569782 INFO @ Sat, 15 Jan 2022 19:46:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:46:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:46:44: #1 tags after filtering in treatment: 7754675 INFO @ Sat, 15 Jan 2022 19:46:44: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 19:46:44: #1 finished! INFO @ Sat, 15 Jan 2022 19:46:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:46:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:46:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:46:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:46:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:46:46: 16000000 INFO @ Sat, 15 Jan 2022 19:46:47: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:46:51: 17000000 INFO @ Sat, 15 Jan 2022 19:46:52: 20000000 INFO @ Sat, 15 Jan 2022 19:46:56: 18000000 INFO @ Sat, 15 Jan 2022 19:46:58: 21000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:47:01: 19000000 INFO @ Sat, 15 Jan 2022 19:47:04: 22000000 INFO @ Sat, 15 Jan 2022 19:47:06: 20000000 INFO @ Sat, 15 Jan 2022 19:47:09: 23000000 INFO @ Sat, 15 Jan 2022 19:47:10: 21000000 INFO @ Sat, 15 Jan 2022 19:47:15: 22000000 INFO @ Sat, 15 Jan 2022 19:47:15: 24000000 INFO @ Sat, 15 Jan 2022 19:47:18: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:47:18: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:47:18: #1 total tags in treatment: 11569782 INFO @ Sat, 15 Jan 2022 19:47:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:47:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:47:18: #1 tags after filtering in treatment: 7754675 INFO @ Sat, 15 Jan 2022 19:47:18: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 19:47:18: #1 finished! INFO @ Sat, 15 Jan 2022 19:47:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:47:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:47:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:47:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:47:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:47:20: 23000000 INFO @ Sat, 15 Jan 2022 19:47:25: 24000000 INFO @ Sat, 15 Jan 2022 19:47:27: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:47:27: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:47:27: #1 total tags in treatment: 11569782 INFO @ Sat, 15 Jan 2022 19:47:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:47:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:47:27: #1 tags after filtering in treatment: 7754675 INFO @ Sat, 15 Jan 2022 19:47:27: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 19:47:27: #1 finished! INFO @ Sat, 15 Jan 2022 19:47:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:47:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:47:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:47:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:47:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781059/SRX11781059.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling