Job ID = 14520516 SRX = SRX11781055 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10165015 spots for SRR15480998/SRR15480998.sra Written 10165015 spots for SRR15480998/SRR15480998.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:48 10165015 reads; of these: 10165015 (100.00%) were paired; of these: 1545412 (15.20%) aligned concordantly 0 times 6904119 (67.92%) aligned concordantly exactly 1 time 1715484 (16.88%) aligned concordantly >1 times ---- 1545412 pairs aligned concordantly 0 times; of these: 297261 (19.24%) aligned discordantly 1 time ---- 1248151 pairs aligned 0 times concordantly or discordantly; of these: 2496302 mates make up the pairs; of these: 1817624 (72.81%) aligned 0 times 424613 (17.01%) aligned exactly 1 time 254065 (10.18%) aligned >1 times 91.06% overall alignment rate Time searching: 00:07:48 Overall time: 00:07:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 571639 / 8869873 = 0.0644 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:31:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:31:48: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:31:48: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:31:54: 1000000 INFO @ Sat, 15 Jan 2022 19:32:01: 2000000 INFO @ Sat, 15 Jan 2022 19:32:07: 3000000 INFO @ Sat, 15 Jan 2022 19:32:13: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:32:19: 5000000 INFO @ Sat, 15 Jan 2022 19:32:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:32:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:32:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:32:26: 6000000 INFO @ Sat, 15 Jan 2022 19:32:28: 1000000 INFO @ Sat, 15 Jan 2022 19:32:33: 7000000 INFO @ Sat, 15 Jan 2022 19:32:34: 2000000 INFO @ Sat, 15 Jan 2022 19:32:40: 8000000 INFO @ Sat, 15 Jan 2022 19:32:40: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:32:46: 4000000 INFO @ Sat, 15 Jan 2022 19:32:47: 9000000 INFO @ Sat, 15 Jan 2022 19:32:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:32:48: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:32:48: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:32:53: 5000000 INFO @ Sat, 15 Jan 2022 19:32:54: 10000000 INFO @ Sat, 15 Jan 2022 19:32:55: 1000000 INFO @ Sat, 15 Jan 2022 19:32:59: 6000000 INFO @ Sat, 15 Jan 2022 19:33:01: 2000000 INFO @ Sat, 15 Jan 2022 19:33:01: 11000000 INFO @ Sat, 15 Jan 2022 19:33:05: 7000000 INFO @ Sat, 15 Jan 2022 19:33:07: 3000000 INFO @ Sat, 15 Jan 2022 19:33:08: 12000000 INFO @ Sat, 15 Jan 2022 19:33:11: 8000000 INFO @ Sat, 15 Jan 2022 19:33:13: 4000000 INFO @ Sat, 15 Jan 2022 19:33:15: 13000000 INFO @ Sat, 15 Jan 2022 19:33:17: 9000000 INFO @ Sat, 15 Jan 2022 19:33:19: 5000000 INFO @ Sat, 15 Jan 2022 19:33:22: 14000000 INFO @ Sat, 15 Jan 2022 19:33:23: 10000000 INFO @ Sat, 15 Jan 2022 19:33:25: 6000000 INFO @ Sat, 15 Jan 2022 19:33:29: 15000000 INFO @ Sat, 15 Jan 2022 19:33:29: 11000000 INFO @ Sat, 15 Jan 2022 19:33:31: 7000000 INFO @ Sat, 15 Jan 2022 19:33:35: 12000000 INFO @ Sat, 15 Jan 2022 19:33:36: 16000000 INFO @ Sat, 15 Jan 2022 19:33:37: 8000000 INFO @ Sat, 15 Jan 2022 19:33:41: 13000000 INFO @ Sat, 15 Jan 2022 19:33:43: 9000000 INFO @ Sat, 15 Jan 2022 19:33:43: 17000000 INFO @ Sat, 15 Jan 2022 19:33:46: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:33:46: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:33:46: #1 total tags in treatment: 8061440 INFO @ Sat, 15 Jan 2022 19:33:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:33:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:33:46: #1 tags after filtering in treatment: 5802397 INFO @ Sat, 15 Jan 2022 19:33:46: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 19:33:46: #1 finished! INFO @ Sat, 15 Jan 2022 19:33:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:33:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:33:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:33:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:33:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:33:47: 14000000 INFO @ Sat, 15 Jan 2022 19:33:49: 10000000 INFO @ Sat, 15 Jan 2022 19:33:53: 15000000 INFO @ Sat, 15 Jan 2022 19:33:55: 11000000 INFO @ Sat, 15 Jan 2022 19:33:59: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:34:00: 12000000 INFO @ Sat, 15 Jan 2022 19:34:05: 17000000 INFO @ Sat, 15 Jan 2022 19:34:06: 13000000 INFO @ Sat, 15 Jan 2022 19:34:07: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:34:07: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:34:07: #1 total tags in treatment: 8061440 INFO @ Sat, 15 Jan 2022 19:34:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:34:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:34:07: #1 tags after filtering in treatment: 5802397 INFO @ Sat, 15 Jan 2022 19:34:07: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 19:34:07: #1 finished! INFO @ Sat, 15 Jan 2022 19:34:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:34:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:34:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:34:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:34:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:34:11: 14000000 INFO @ Sat, 15 Jan 2022 19:34:16: 15000000 INFO @ Sat, 15 Jan 2022 19:34:21: 16000000 INFO @ Sat, 15 Jan 2022 19:34:27: 17000000 INFO @ Sat, 15 Jan 2022 19:34:28: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:34:28: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:34:28: #1 total tags in treatment: 8061440 INFO @ Sat, 15 Jan 2022 19:34:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:34:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:34:29: #1 tags after filtering in treatment: 5802397 INFO @ Sat, 15 Jan 2022 19:34:29: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 19:34:29: #1 finished! INFO @ Sat, 15 Jan 2022 19:34:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:34:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:34:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:34:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:34:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781055/SRX11781055.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling