Job ID = 14520512 SRX = SRX11781051 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:15:53 prefetch.2.10.7: 1) Downloading 'SRR15480994'... 2022-01-15T10:15:53 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:17:27 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:17:27 prefetch.2.10.7: 1) 'SRR15480994' was downloaded successfully 2022-01-15T10:17:27 prefetch.2.10.7: 'SRR15480994' has 0 unresolved dependencies Read 12858178 spots for SRR15480994/SRR15480994.sra Written 12858178 spots for SRR15480994/SRR15480994.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:47 12858178 reads; of these: 12858178 (100.00%) were paired; of these: 1405117 (10.93%) aligned concordantly 0 times 9182378 (71.41%) aligned concordantly exactly 1 time 2270683 (17.66%) aligned concordantly >1 times ---- 1405117 pairs aligned concordantly 0 times; of these: 297074 (21.14%) aligned discordantly 1 time ---- 1108043 pairs aligned 0 times concordantly or discordantly; of these: 2216086 mates make up the pairs; of these: 1636764 (73.86%) aligned 0 times 340070 (15.35%) aligned exactly 1 time 239252 (10.80%) aligned >1 times 93.64% overall alignment rate Time searching: 00:09:47 Overall time: 00:09:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 791670 / 11703616 = 0.0676 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:36:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:36:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:36:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:36:26: 1000000 INFO @ Sat, 15 Jan 2022 19:36:33: 2000000 INFO @ Sat, 15 Jan 2022 19:36:40: 3000000 INFO @ Sat, 15 Jan 2022 19:36:47: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:36:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:36:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:36:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:36:54: 5000000 INFO @ Sat, 15 Jan 2022 19:36:56: 1000000 INFO @ Sat, 15 Jan 2022 19:37:02: 6000000 INFO @ Sat, 15 Jan 2022 19:37:04: 2000000 INFO @ Sat, 15 Jan 2022 19:37:09: 7000000 INFO @ Sat, 15 Jan 2022 19:37:11: 3000000 INFO @ Sat, 15 Jan 2022 19:37:16: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:37:18: 4000000 INFO @ Sat, 15 Jan 2022 19:37:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:37:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:37:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:37:23: 9000000 INFO @ Sat, 15 Jan 2022 19:37:25: 5000000 INFO @ Sat, 15 Jan 2022 19:37:27: 1000000 INFO @ Sat, 15 Jan 2022 19:37:31: 10000000 INFO @ Sat, 15 Jan 2022 19:37:33: 6000000 INFO @ Sat, 15 Jan 2022 19:37:35: 2000000 INFO @ Sat, 15 Jan 2022 19:37:38: 11000000 INFO @ Sat, 15 Jan 2022 19:37:40: 7000000 INFO @ Sat, 15 Jan 2022 19:37:42: 3000000 INFO @ Sat, 15 Jan 2022 19:37:45: 12000000 INFO @ Sat, 15 Jan 2022 19:37:48: 8000000 INFO @ Sat, 15 Jan 2022 19:37:50: 4000000 INFO @ Sat, 15 Jan 2022 19:37:52: 13000000 INFO @ Sat, 15 Jan 2022 19:37:55: 9000000 INFO @ Sat, 15 Jan 2022 19:37:58: 5000000 INFO @ Sat, 15 Jan 2022 19:38:00: 14000000 INFO @ Sat, 15 Jan 2022 19:38:02: 10000000 INFO @ Sat, 15 Jan 2022 19:38:06: 6000000 INFO @ Sat, 15 Jan 2022 19:38:07: 15000000 INFO @ Sat, 15 Jan 2022 19:38:09: 11000000 INFO @ Sat, 15 Jan 2022 19:38:14: 7000000 INFO @ Sat, 15 Jan 2022 19:38:14: 16000000 INFO @ Sat, 15 Jan 2022 19:38:16: 12000000 INFO @ Sat, 15 Jan 2022 19:38:21: 17000000 INFO @ Sat, 15 Jan 2022 19:38:21: 8000000 INFO @ Sat, 15 Jan 2022 19:38:24: 13000000 INFO @ Sat, 15 Jan 2022 19:38:28: 18000000 INFO @ Sat, 15 Jan 2022 19:38:29: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:38:31: 14000000 INFO @ Sat, 15 Jan 2022 19:38:36: 19000000 INFO @ Sat, 15 Jan 2022 19:38:37: 10000000 INFO @ Sat, 15 Jan 2022 19:38:38: 15000000 INFO @ Sat, 15 Jan 2022 19:38:43: 20000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:38:44: 11000000 INFO @ Sat, 15 Jan 2022 19:38:46: 16000000 INFO @ Sat, 15 Jan 2022 19:38:50: 21000000 INFO @ Sat, 15 Jan 2022 19:38:52: 12000000 INFO @ Sat, 15 Jan 2022 19:38:53: 17000000 INFO @ Sat, 15 Jan 2022 19:38:57: 22000000 INFO @ Sat, 15 Jan 2022 19:38:59: 13000000 INFO @ Sat, 15 Jan 2022 19:39:00: 18000000 INFO @ Sat, 15 Jan 2022 19:39:01: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:39:01: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:39:01: #1 total tags in treatment: 10672805 INFO @ Sat, 15 Jan 2022 19:39:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:39:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:39:01: #1 tags after filtering in treatment: 7400739 INFO @ Sat, 15 Jan 2022 19:39:01: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 19:39:01: #1 finished! INFO @ Sat, 15 Jan 2022 19:39:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:39:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:39:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:39:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:39:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:39:07: 14000000 INFO @ Sat, 15 Jan 2022 19:39:08: 19000000 INFO @ Sat, 15 Jan 2022 19:39:14: 15000000 INFO @ Sat, 15 Jan 2022 19:39:15: 20000000 INFO @ Sat, 15 Jan 2022 19:39:22: 16000000 INFO @ Sat, 15 Jan 2022 19:39:23: 21000000 INFO @ Sat, 15 Jan 2022 19:39:29: 17000000 INFO @ Sat, 15 Jan 2022 19:39:31: 22000000 INFO @ Sat, 15 Jan 2022 19:39:34: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:39:34: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:39:34: #1 total tags in treatment: 10672805 INFO @ Sat, 15 Jan 2022 19:39:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:39:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:39:34: #1 tags after filtering in treatment: 7400739 INFO @ Sat, 15 Jan 2022 19:39:34: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 19:39:34: #1 finished! INFO @ Sat, 15 Jan 2022 19:39:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:39:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:39:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:39:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:39:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:39:36: 18000000 INFO @ Sat, 15 Jan 2022 19:39:43: 19000000 INFO @ Sat, 15 Jan 2022 19:39:50: 20000000 INFO @ Sat, 15 Jan 2022 19:39:57: 21000000 INFO @ Sat, 15 Jan 2022 19:40:03: 22000000 INFO @ Sat, 15 Jan 2022 19:40:07: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:40:07: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:40:07: #1 total tags in treatment: 10672805 INFO @ Sat, 15 Jan 2022 19:40:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:40:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:40:07: #1 tags after filtering in treatment: 7400739 INFO @ Sat, 15 Jan 2022 19:40:07: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 19:40:07: #1 finished! INFO @ Sat, 15 Jan 2022 19:40:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:40:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:40:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:40:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:40:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781051/SRX11781051.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling