Job ID = 14520481 SRX = SRX11781045 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:12:24 prefetch.2.10.7: 1) Downloading 'SRR15480988'... 2022-01-15T10:12:24 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:13:06 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:13:06 prefetch.2.10.7: 1) 'SRR15480988' was downloaded successfully 2022-01-15T10:13:06 prefetch.2.10.7: 'SRR15480988' has 0 unresolved dependencies Read 8713099 spots for SRR15480988/SRR15480988.sra Written 8713099 spots for SRR15480988/SRR15480988.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:15 8713099 reads; of these: 8713099 (100.00%) were paired; of these: 1356914 (15.57%) aligned concordantly 0 times 5388421 (61.84%) aligned concordantly exactly 1 time 1967764 (22.58%) aligned concordantly >1 times ---- 1356914 pairs aligned concordantly 0 times; of these: 223962 (16.51%) aligned discordantly 1 time ---- 1132952 pairs aligned 0 times concordantly or discordantly; of these: 2265904 mates make up the pairs; of these: 1564017 (69.02%) aligned 0 times 442968 (19.55%) aligned exactly 1 time 258919 (11.43%) aligned >1 times 91.02% overall alignment rate Time searching: 00:10:15 Overall time: 00:10:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 797989 / 7538309 = 0.1059 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:31:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:31:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:31:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:31:19: 1000000 INFO @ Sat, 15 Jan 2022 19:31:27: 2000000 INFO @ Sat, 15 Jan 2022 19:31:34: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:31:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:31:42: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:31:42: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:31:42: 4000000 INFO @ Sat, 15 Jan 2022 19:31:49: 1000000 INFO @ Sat, 15 Jan 2022 19:31:50: 5000000 INFO @ Sat, 15 Jan 2022 19:31:57: 2000000 INFO @ Sat, 15 Jan 2022 19:31:57: 6000000 INFO @ Sat, 15 Jan 2022 19:32:04: 3000000 INFO @ Sat, 15 Jan 2022 19:32:05: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:32:11: 4000000 INFO @ Sat, 15 Jan 2022 19:32:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:32:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:32:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:32:13: 8000000 INFO @ Sat, 15 Jan 2022 19:32:19: 5000000 INFO @ Sat, 15 Jan 2022 19:32:19: 1000000 INFO @ Sat, 15 Jan 2022 19:32:22: 9000000 INFO @ Sat, 15 Jan 2022 19:32:26: 6000000 INFO @ Sat, 15 Jan 2022 19:32:26: 2000000 INFO @ Sat, 15 Jan 2022 19:32:31: 10000000 INFO @ Sat, 15 Jan 2022 19:32:33: 7000000 INFO @ Sat, 15 Jan 2022 19:32:34: 3000000 INFO @ Sat, 15 Jan 2022 19:32:39: 11000000 INFO @ Sat, 15 Jan 2022 19:32:41: 8000000 INFO @ Sat, 15 Jan 2022 19:32:42: 4000000 INFO @ Sat, 15 Jan 2022 19:32:48: 12000000 INFO @ Sat, 15 Jan 2022 19:32:48: 9000000 INFO @ Sat, 15 Jan 2022 19:32:49: 5000000 INFO @ Sat, 15 Jan 2022 19:32:55: 10000000 INFO @ Sat, 15 Jan 2022 19:32:57: 13000000 INFO @ Sat, 15 Jan 2022 19:32:57: 6000000 INFO @ Sat, 15 Jan 2022 19:33:02: 11000000 INFO @ Sat, 15 Jan 2022 19:33:05: 7000000 INFO @ Sat, 15 Jan 2022 19:33:05: 14000000 INFO @ Sat, 15 Jan 2022 19:33:08: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:33:08: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:33:08: #1 total tags in treatment: 6569013 INFO @ Sat, 15 Jan 2022 19:33:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:33:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:33:08: #1 tags after filtering in treatment: 4527831 INFO @ Sat, 15 Jan 2022 19:33:08: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 19:33:08: #1 finished! INFO @ Sat, 15 Jan 2022 19:33:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:33:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:33:08: #2 number of paired peaks: 45 WARNING @ Sat, 15 Jan 2022 19:33:08: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:33:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:33:09: 12000000 INFO @ Sat, 15 Jan 2022 19:33:12: 8000000 INFO @ Sat, 15 Jan 2022 19:33:16: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:33:20: 9000000 INFO @ Sat, 15 Jan 2022 19:33:24: 14000000 INFO @ Sat, 15 Jan 2022 19:33:26: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:33:26: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:33:26: #1 total tags in treatment: 6569013 INFO @ Sat, 15 Jan 2022 19:33:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:33:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:33:26: #1 tags after filtering in treatment: 4527831 INFO @ Sat, 15 Jan 2022 19:33:26: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 19:33:26: #1 finished! INFO @ Sat, 15 Jan 2022 19:33:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:33:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:33:26: #2 number of paired peaks: 45 WARNING @ Sat, 15 Jan 2022 19:33:26: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:33:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:33:26: 10000000 INFO @ Sat, 15 Jan 2022 19:33:33: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:33:40: 12000000 INFO @ Sat, 15 Jan 2022 19:33:47: 13000000 INFO @ Sat, 15 Jan 2022 19:33:54: 14000000 INFO @ Sat, 15 Jan 2022 19:33:56: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:33:56: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:33:56: #1 total tags in treatment: 6569013 INFO @ Sat, 15 Jan 2022 19:33:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:33:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:33:56: #1 tags after filtering in treatment: 4527831 INFO @ Sat, 15 Jan 2022 19:33:56: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 19:33:56: #1 finished! INFO @ Sat, 15 Jan 2022 19:33:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:33:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:33:56: #2 number of paired peaks: 45 WARNING @ Sat, 15 Jan 2022 19:33:56: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:33:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781045/SRX11781045.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling