Job ID = 14520453 SRX = SRX11781033 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:07:51 prefetch.2.10.7: 1) Downloading 'SRR15480976'... 2022-01-15T10:07:51 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:08:30 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:08:30 prefetch.2.10.7: 1) 'SRR15480976' was downloaded successfully 2022-01-15T10:08:30 prefetch.2.10.7: 'SRR15480976' has 0 unresolved dependencies Read 9795262 spots for SRR15480976/SRR15480976.sra Written 9795262 spots for SRR15480976/SRR15480976.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:48 9795262 reads; of these: 9795262 (100.00%) were paired; of these: 1188601 (12.13%) aligned concordantly 0 times 6780181 (69.22%) aligned concordantly exactly 1 time 1826480 (18.65%) aligned concordantly >1 times ---- 1188601 pairs aligned concordantly 0 times; of these: 233771 (19.67%) aligned discordantly 1 time ---- 954830 pairs aligned 0 times concordantly or discordantly; of these: 1909660 mates make up the pairs; of these: 1411468 (73.91%) aligned 0 times 279188 (14.62%) aligned exactly 1 time 219004 (11.47%) aligned >1 times 92.80% overall alignment rate Time searching: 00:07:48 Overall time: 00:07:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 592112 / 8802582 = 0.0673 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:24:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:24:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:24:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:24:07: 1000000 INFO @ Sat, 15 Jan 2022 19:24:12: 2000000 INFO @ Sat, 15 Jan 2022 19:24:18: 3000000 INFO @ Sat, 15 Jan 2022 19:24:23: 4000000 INFO @ Sat, 15 Jan 2022 19:24:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:24:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:24:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:24:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:24:34: 6000000 INFO @ Sat, 15 Jan 2022 19:24:38: 1000000 INFO @ Sat, 15 Jan 2022 19:24:40: 7000000 INFO @ Sat, 15 Jan 2022 19:24:44: 2000000 INFO @ Sat, 15 Jan 2022 19:24:46: 8000000 INFO @ Sat, 15 Jan 2022 19:24:50: 3000000 INFO @ Sat, 15 Jan 2022 19:24:52: 9000000 INFO @ Sat, 15 Jan 2022 19:24:56: 4000000 INFO @ Sat, 15 Jan 2022 19:24:58: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:25:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:25:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:25:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:25:02: 5000000 INFO @ Sat, 15 Jan 2022 19:25:04: 11000000 INFO @ Sat, 15 Jan 2022 19:25:08: 6000000 INFO @ Sat, 15 Jan 2022 19:25:09: 1000000 INFO @ Sat, 15 Jan 2022 19:25:11: 12000000 INFO @ Sat, 15 Jan 2022 19:25:15: 7000000 INFO @ Sat, 15 Jan 2022 19:25:17: 2000000 INFO @ Sat, 15 Jan 2022 19:25:17: 13000000 INFO @ Sat, 15 Jan 2022 19:25:21: 8000000 INFO @ Sat, 15 Jan 2022 19:25:24: 14000000 INFO @ Sat, 15 Jan 2022 19:25:24: 3000000 INFO @ Sat, 15 Jan 2022 19:25:28: 9000000 INFO @ Sat, 15 Jan 2022 19:25:31: 15000000 INFO @ Sat, 15 Jan 2022 19:25:32: 4000000 INFO @ Sat, 15 Jan 2022 19:25:35: 10000000 INFO @ Sat, 15 Jan 2022 19:25:37: 16000000 INFO @ Sat, 15 Jan 2022 19:25:39: 5000000 INFO @ Sat, 15 Jan 2022 19:25:42: 11000000 INFO @ Sat, 15 Jan 2022 19:25:44: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:25:44: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:25:44: #1 total tags in treatment: 8024550 INFO @ Sat, 15 Jan 2022 19:25:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:25:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:25:44: #1 tags after filtering in treatment: 5592489 INFO @ Sat, 15 Jan 2022 19:25:44: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 19:25:44: #1 finished! INFO @ Sat, 15 Jan 2022 19:25:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:25:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:25:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:25:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:25:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:25:46: 6000000 INFO @ Sat, 15 Jan 2022 19:25:48: 12000000 INFO @ Sat, 15 Jan 2022 19:25:53: 7000000 INFO @ Sat, 15 Jan 2022 19:25:55: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:26:00: 8000000 INFO @ Sat, 15 Jan 2022 19:26:02: 14000000 INFO @ Sat, 15 Jan 2022 19:26:07: 9000000 INFO @ Sat, 15 Jan 2022 19:26:08: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:26:14: 10000000 INFO @ Sat, 15 Jan 2022 19:26:15: 16000000 INFO @ Sat, 15 Jan 2022 19:26:21: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:26:21: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:26:21: #1 total tags in treatment: 8024550 INFO @ Sat, 15 Jan 2022 19:26:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:26:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:26:22: #1 tags after filtering in treatment: 5592489 INFO @ Sat, 15 Jan 2022 19:26:22: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 19:26:22: #1 finished! INFO @ Sat, 15 Jan 2022 19:26:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:26:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:26:22: 11000000 INFO @ Sat, 15 Jan 2022 19:26:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:26:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:26:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:26:28: 12000000 INFO @ Sat, 15 Jan 2022 19:26:35: 13000000 INFO @ Sat, 15 Jan 2022 19:26:42: 14000000 INFO @ Sat, 15 Jan 2022 19:26:48: 15000000 INFO @ Sat, 15 Jan 2022 19:26:55: 16000000 INFO @ Sat, 15 Jan 2022 19:27:02: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:27:02: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:27:02: #1 total tags in treatment: 8024550 INFO @ Sat, 15 Jan 2022 19:27:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:27:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:27:02: #1 tags after filtering in treatment: 5592489 INFO @ Sat, 15 Jan 2022 19:27:02: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 19:27:02: #1 finished! INFO @ Sat, 15 Jan 2022 19:27:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:27:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:27:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:27:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:27:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781033/SRX11781033.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling