Job ID = 14520452 SRX = SRX11781032 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:07:51 prefetch.2.10.7: 1) Downloading 'SRR15480975'... 2022-01-15T10:07:51 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:08:13 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:08:13 prefetch.2.10.7: 'SRR15480975' is valid 2022-01-15T10:08:13 prefetch.2.10.7: 1) 'SRR15480975' was downloaded successfully 2022-01-15T10:08:13 prefetch.2.10.7: 'SRR15480975' has 0 unresolved dependencies Read 4988670 spots for SRR15480975/SRR15480975.sra Written 4988670 spots for SRR15480975/SRR15480975.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:09 4988670 reads; of these: 4988670 (100.00%) were paired; of these: 3006083 (60.26%) aligned concordantly 0 times 1921976 (38.53%) aligned concordantly exactly 1 time 60611 (1.21%) aligned concordantly >1 times ---- 3006083 pairs aligned concordantly 0 times; of these: 166153 (5.53%) aligned discordantly 1 time ---- 2839930 pairs aligned 0 times concordantly or discordantly; of these: 5679860 mates make up the pairs; of these: 3488032 (61.41%) aligned 0 times 2134425 (37.58%) aligned exactly 1 time 57403 (1.01%) aligned >1 times 65.04% overall alignment rate Time searching: 00:03:09 Overall time: 00:03:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 486405 / 2136663 = 0.2276 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:14:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:14:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:14:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:14:36: 1000000 INFO @ Sat, 15 Jan 2022 19:14:41: 2000000 INFO @ Sat, 15 Jan 2022 19:14:45: 3000000 INFO @ Sat, 15 Jan 2022 19:14:50: 4000000 INFO @ Sat, 15 Jan 2022 19:14:54: 5000000 INFO @ Sat, 15 Jan 2022 19:14:56: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:14:56: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:14:56: #1 total tags in treatment: 1529658 INFO @ Sat, 15 Jan 2022 19:14:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:14:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:14:56: #1 tags after filtering in treatment: 169463 INFO @ Sat, 15 Jan 2022 19:14:56: #1 Redundant rate of treatment: 0.89 INFO @ Sat, 15 Jan 2022 19:14:56: #1 finished! INFO @ Sat, 15 Jan 2022 19:14:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:14:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:14:56: #2 number of paired peaks: 24 WARNING @ Sat, 15 Jan 2022 19:14:56: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:14:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:15:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:15:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:15:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:15:06: 1000000 INFO @ Sat, 15 Jan 2022 19:15:11: 2000000 INFO @ Sat, 15 Jan 2022 19:15:15: 3000000 INFO @ Sat, 15 Jan 2022 19:15:20: 4000000 INFO @ Sat, 15 Jan 2022 19:15:24: 5000000 INFO @ Sat, 15 Jan 2022 19:15:26: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:15:26: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:15:26: #1 total tags in treatment: 1529658 INFO @ Sat, 15 Jan 2022 19:15:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:15:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:15:27: #1 tags after filtering in treatment: 169463 INFO @ Sat, 15 Jan 2022 19:15:27: #1 Redundant rate of treatment: 0.89 INFO @ Sat, 15 Jan 2022 19:15:27: #1 finished! INFO @ Sat, 15 Jan 2022 19:15:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:15:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:15:27: #2 number of paired peaks: 24 WARNING @ Sat, 15 Jan 2022 19:15:27: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:15:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:15:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:15:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:15:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:15:36: 1000000 INFO @ Sat, 15 Jan 2022 19:15:41: 2000000 INFO @ Sat, 15 Jan 2022 19:15:46: 3000000 BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:15:50: 4000000 INFO @ Sat, 15 Jan 2022 19:15:55: 5000000 INFO @ Sat, 15 Jan 2022 19:15:57: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:15:57: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:15:57: #1 total tags in treatment: 1529658 INFO @ Sat, 15 Jan 2022 19:15:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:15:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:15:57: #1 tags after filtering in treatment: 169463 INFO @ Sat, 15 Jan 2022 19:15:57: #1 Redundant rate of treatment: 0.89 INFO @ Sat, 15 Jan 2022 19:15:57: #1 finished! INFO @ Sat, 15 Jan 2022 19:15:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:15:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:15:57: #2 number of paired peaks: 24 WARNING @ Sat, 15 Jan 2022 19:15:57: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:15:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781032/SRX11781032.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling