Job ID = 14520434 SRX = SRX11781029 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:05:36 prefetch.2.10.7: 1) Downloading 'SRR15480972'... 2022-01-15T10:05:36 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:06:12 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:06:13 prefetch.2.10.7: 'SRR15480972' is valid 2022-01-15T10:06:13 prefetch.2.10.7: 1) 'SRR15480972' was downloaded successfully 2022-01-15T10:06:13 prefetch.2.10.7: 'SRR15480972' has 0 unresolved dependencies Read 8679370 spots for SRR15480972/SRR15480972.sra Written 8679370 spots for SRR15480972/SRR15480972.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:34 8679370 reads; of these: 8679370 (100.00%) were paired; of these: 1403452 (16.17%) aligned concordantly 0 times 6027161 (69.44%) aligned concordantly exactly 1 time 1248757 (14.39%) aligned concordantly >1 times ---- 1403452 pairs aligned concordantly 0 times; of these: 470106 (33.50%) aligned discordantly 1 time ---- 933346 pairs aligned 0 times concordantly or discordantly; of these: 1866692 mates make up the pairs; of these: 1557537 (83.44%) aligned 0 times 108024 (5.79%) aligned exactly 1 time 201131 (10.77%) aligned >1 times 91.03% overall alignment rate Time searching: 00:06:34 Overall time: 00:06:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 217671 / 7700393 = 0.0283 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:18:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:18:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:18:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:18:44: 1000000 INFO @ Sat, 15 Jan 2022 19:18:50: 2000000 INFO @ Sat, 15 Jan 2022 19:18:57: 3000000 INFO @ Sat, 15 Jan 2022 19:19:03: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:19:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:19:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:19:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:19:10: 5000000 INFO @ Sat, 15 Jan 2022 19:19:14: 1000000 INFO @ Sat, 15 Jan 2022 19:19:17: 6000000 INFO @ Sat, 15 Jan 2022 19:19:21: 2000000 INFO @ Sat, 15 Jan 2022 19:19:24: 7000000 INFO @ Sat, 15 Jan 2022 19:19:28: 3000000 INFO @ Sat, 15 Jan 2022 19:19:31: 8000000 INFO @ Sat, 15 Jan 2022 19:19:35: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:19:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:19:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:19:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:19:38: 9000000 INFO @ Sat, 15 Jan 2022 19:19:42: 5000000 INFO @ Sat, 15 Jan 2022 19:19:44: 1000000 INFO @ Sat, 15 Jan 2022 19:19:45: 10000000 INFO @ Sat, 15 Jan 2022 19:19:49: 6000000 INFO @ Sat, 15 Jan 2022 19:19:51: 2000000 INFO @ Sat, 15 Jan 2022 19:19:53: 11000000 INFO @ Sat, 15 Jan 2022 19:19:56: 7000000 INFO @ Sat, 15 Jan 2022 19:19:58: 3000000 INFO @ Sat, 15 Jan 2022 19:20:00: 12000000 INFO @ Sat, 15 Jan 2022 19:20:03: 8000000 INFO @ Sat, 15 Jan 2022 19:20:05: 4000000 INFO @ Sat, 15 Jan 2022 19:20:07: 13000000 INFO @ Sat, 15 Jan 2022 19:20:10: 9000000 INFO @ Sat, 15 Jan 2022 19:20:12: 5000000 INFO @ Sat, 15 Jan 2022 19:20:14: 14000000 INFO @ Sat, 15 Jan 2022 19:20:18: 10000000 INFO @ Sat, 15 Jan 2022 19:20:19: 6000000 INFO @ Sat, 15 Jan 2022 19:20:21: 15000000 INFO @ Sat, 15 Jan 2022 19:20:23: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:20:23: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:20:23: #1 total tags in treatment: 7072681 INFO @ Sat, 15 Jan 2022 19:20:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:20:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:20:23: #1 tags after filtering in treatment: 5340564 INFO @ Sat, 15 Jan 2022 19:20:23: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 19:20:23: #1 finished! INFO @ Sat, 15 Jan 2022 19:20:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:20:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:20:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:20:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:20:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:20:25: 11000000 INFO @ Sat, 15 Jan 2022 19:20:26: 7000000 INFO @ Sat, 15 Jan 2022 19:20:32: 12000000 INFO @ Sat, 15 Jan 2022 19:20:33: 8000000 INFO @ Sat, 15 Jan 2022 19:20:39: 13000000 INFO @ Sat, 15 Jan 2022 19:20:40: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:20:46: 14000000 INFO @ Sat, 15 Jan 2022 19:20:47: 10000000 INFO @ Sat, 15 Jan 2022 19:20:53: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:20:53: 11000000 INFO @ Sat, 15 Jan 2022 19:20:55: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:20:55: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:20:55: #1 total tags in treatment: 7072681 INFO @ Sat, 15 Jan 2022 19:20:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:20:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:20:55: #1 tags after filtering in treatment: 5340564 INFO @ Sat, 15 Jan 2022 19:20:55: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 19:20:55: #1 finished! INFO @ Sat, 15 Jan 2022 19:20:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:20:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:20:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:20:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:20:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:21:00: 12000000 INFO @ Sat, 15 Jan 2022 19:21:07: 13000000 INFO @ Sat, 15 Jan 2022 19:21:13: 14000000 INFO @ Sat, 15 Jan 2022 19:21:20: 15000000 INFO @ Sat, 15 Jan 2022 19:21:22: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:21:22: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:21:22: #1 total tags in treatment: 7072681 INFO @ Sat, 15 Jan 2022 19:21:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:21:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:21:22: #1 tags after filtering in treatment: 5340564 INFO @ Sat, 15 Jan 2022 19:21:22: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 19:21:22: #1 finished! INFO @ Sat, 15 Jan 2022 19:21:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:21:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:21:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:21:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:21:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781029/SRX11781029.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling