Job ID = 14520424 SRX = SRX11781021 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T10:04:21 prefetch.2.10.7: 1) Downloading 'SRR15480964'... 2022-01-15T10:04:21 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T10:04:55 prefetch.2.10.7: HTTPS download succeed 2022-01-15T10:04:56 prefetch.2.10.7: 'SRR15480964' is valid 2022-01-15T10:04:56 prefetch.2.10.7: 1) 'SRR15480964' was downloaded successfully 2022-01-15T10:04:56 prefetch.2.10.7: 'SRR15480964' has 0 unresolved dependencies Read 7775746 spots for SRR15480964/SRR15480964.sra Written 7775746 spots for SRR15480964/SRR15480964.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:32 7775746 reads; of these: 7775746 (100.00%) were paired; of these: 1265177 (16.27%) aligned concordantly 0 times 4839991 (62.24%) aligned concordantly exactly 1 time 1670578 (21.48%) aligned concordantly >1 times ---- 1265177 pairs aligned concordantly 0 times; of these: 418146 (33.05%) aligned discordantly 1 time ---- 847031 pairs aligned 0 times concordantly or discordantly; of these: 1694062 mates make up the pairs; of these: 1324030 (78.16%) aligned 0 times 91521 (5.40%) aligned exactly 1 time 278511 (16.44%) aligned >1 times 91.49% overall alignment rate Time searching: 00:08:32 Overall time: 00:08:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 354883 / 6884647 = 0.0515 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:19:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:19:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:19:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:20:07: 1000000 INFO @ Sat, 15 Jan 2022 19:20:17: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:20:26: 3000000 INFO @ Sat, 15 Jan 2022 19:20:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:20:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:20:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:20:35: 4000000 INFO @ Sat, 15 Jan 2022 19:20:38: 1000000 INFO @ Sat, 15 Jan 2022 19:20:45: 5000000 INFO @ Sat, 15 Jan 2022 19:20:47: 2000000 INFO @ Sat, 15 Jan 2022 19:20:54: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:20:56: 3000000 INFO @ Sat, 15 Jan 2022 19:20:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:20:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:20:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:21:04: 7000000 INFO @ Sat, 15 Jan 2022 19:21:05: 4000000 INFO @ Sat, 15 Jan 2022 19:21:06: 1000000 INFO @ Sat, 15 Jan 2022 19:21:13: 8000000 INFO @ Sat, 15 Jan 2022 19:21:14: 5000000 INFO @ Sat, 15 Jan 2022 19:21:15: 2000000 INFO @ Sat, 15 Jan 2022 19:21:23: 9000000 INFO @ Sat, 15 Jan 2022 19:21:25: 3000000 INFO @ Sat, 15 Jan 2022 19:21:25: 6000000 INFO @ Sat, 15 Jan 2022 19:21:34: 10000000 INFO @ Sat, 15 Jan 2022 19:21:34: 4000000 INFO @ Sat, 15 Jan 2022 19:21:36: 7000000 INFO @ Sat, 15 Jan 2022 19:21:44: 5000000 INFO @ Sat, 15 Jan 2022 19:21:45: 11000000 INFO @ Sat, 15 Jan 2022 19:21:46: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:21:55: 6000000 INFO @ Sat, 15 Jan 2022 19:21:55: 12000000 INFO @ Sat, 15 Jan 2022 19:21:57: 9000000 INFO @ Sat, 15 Jan 2022 19:22:05: 7000000 INFO @ Sat, 15 Jan 2022 19:22:08: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:22:10: 10000000 INFO @ Sat, 15 Jan 2022 19:22:12: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:22:12: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:22:12: #1 total tags in treatment: 6169181 INFO @ Sat, 15 Jan 2022 19:22:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:22:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:22:13: #1 tags after filtering in treatment: 4447711 INFO @ Sat, 15 Jan 2022 19:22:13: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 19:22:13: #1 finished! INFO @ Sat, 15 Jan 2022 19:22:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:22:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:22:13: #2 number of paired peaks: 42 WARNING @ Sat, 15 Jan 2022 19:22:13: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:22:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:22:16: 8000000 INFO @ Sat, 15 Jan 2022 19:22:18: 11000000 INFO @ Sat, 15 Jan 2022 19:22:26: 9000000 INFO @ Sat, 15 Jan 2022 19:22:28: 12000000 INFO @ Sat, 15 Jan 2022 19:22:35: 10000000 INFO @ Sat, 15 Jan 2022 19:22:38: 13000000 INFO @ Sat, 15 Jan 2022 19:22:43: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:22:43: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:22:43: #1 total tags in treatment: 6169181 INFO @ Sat, 15 Jan 2022 19:22:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:22:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:22:43: #1 tags after filtering in treatment: 4447711 INFO @ Sat, 15 Jan 2022 19:22:43: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 19:22:43: #1 finished! INFO @ Sat, 15 Jan 2022 19:22:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:22:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:22:43: #2 number of paired peaks: 42 WARNING @ Sat, 15 Jan 2022 19:22:43: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:22:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:22:44: 11000000 INFO @ Sat, 15 Jan 2022 19:22:53: 12000000 INFO @ Sat, 15 Jan 2022 19:23:02: 13000000 INFO @ Sat, 15 Jan 2022 19:23:06: #1 tag size is determined as 80 bps INFO @ Sat, 15 Jan 2022 19:23:06: #1 tag size = 80 INFO @ Sat, 15 Jan 2022 19:23:06: #1 total tags in treatment: 6169181 INFO @ Sat, 15 Jan 2022 19:23:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:23:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:23:06: #1 tags after filtering in treatment: 4447711 INFO @ Sat, 15 Jan 2022 19:23:06: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 19:23:06: #1 finished! INFO @ Sat, 15 Jan 2022 19:23:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:23:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:23:06: #2 number of paired peaks: 42 WARNING @ Sat, 15 Jan 2022 19:23:06: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:23:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11781021/SRX11781021.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling