Job ID = 9161866 sra ファイルのダウンロード中... Completed: 413895K bytes transferred in 6 seconds (524159K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 16487242 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1166300/SRR2187899.sra Written 16487242 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:10 16487242 reads; of these: 16487242 (100.00%) were unpaired; of these: 1429916 (8.67%) aligned 0 times 12967006 (78.65%) aligned exactly 1 time 2090320 (12.68%) aligned >1 times 91.33% overall alignment rate Time searching: 00:03:10 Overall time: 00:03:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8833829 / 15057326 = 0.5867 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:55:41: # Command line: callpeak -t SRX1166300.bam -f BAM -g 12100000 -n SRX1166300.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1166300.20 # format = BAM # ChIP-seq file = ['SRX1166300.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:55:41: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:55:41: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:55:41: # Command line: callpeak -t SRX1166300.bam -f BAM -g 12100000 -n SRX1166300.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1166300.10 # format = BAM # ChIP-seq file = ['SRX1166300.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:55:41: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:55:41: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:55:41: # Command line: callpeak -t SRX1166300.bam -f BAM -g 12100000 -n SRX1166300.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1166300.05 # format = BAM # ChIP-seq file = ['SRX1166300.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:55:41: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:55:41: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:55:48: 1000000 INFO @ Wed, 28 Jun 2017 04:55:48: 1000000 INFO @ Wed, 28 Jun 2017 04:55:48: 1000000 INFO @ Wed, 28 Jun 2017 04:55:54: 2000000 INFO @ Wed, 28 Jun 2017 04:55:55: 2000000 INFO @ Wed, 28 Jun 2017 04:55:55: 2000000 INFO @ Wed, 28 Jun 2017 04:56:01: 3000000 INFO @ Wed, 28 Jun 2017 04:56:02: 3000000 INFO @ Wed, 28 Jun 2017 04:56:02: 3000000 INFO @ Wed, 28 Jun 2017 04:56:08: 4000000 INFO @ Wed, 28 Jun 2017 04:56:09: 4000000 INFO @ Wed, 28 Jun 2017 04:56:09: 4000000 INFO @ Wed, 28 Jun 2017 04:56:15: 5000000 INFO @ Wed, 28 Jun 2017 04:56:17: 5000000 INFO @ Wed, 28 Jun 2017 04:56:17: 5000000 INFO @ Wed, 28 Jun 2017 04:56:22: 6000000 INFO @ Wed, 28 Jun 2017 04:56:23: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:56:23: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:56:23: #1 total tags in treatment: 6223497 INFO @ Wed, 28 Jun 2017 04:56:23: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:56:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:56:23: #1 tags after filtering in treatment: 6223497 INFO @ Wed, 28 Jun 2017 04:56:23: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:56:23: #1 finished! INFO @ Wed, 28 Jun 2017 04:56:23: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:56:23: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:56:23: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:56:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:56:23: Process for pairing-model is terminated! cat: SRX1166300.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1166300.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1166300.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1166300.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:56:24: 6000000 INFO @ Wed, 28 Jun 2017 04:56:24: 6000000 INFO @ Wed, 28 Jun 2017 04:56:25: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:56:25: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:56:25: #1 total tags in treatment: 6223497 INFO @ Wed, 28 Jun 2017 04:56:25: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:56:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:56:25: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:56:25: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:56:25: #1 total tags in treatment: 6223497 INFO @ Wed, 28 Jun 2017 04:56:25: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:56:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:56:25: #1 tags after filtering in treatment: 6223497 INFO @ Wed, 28 Jun 2017 04:56:25: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:56:25: #1 finished! INFO @ Wed, 28 Jun 2017 04:56:25: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:56:25: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:56:25: #1 tags after filtering in treatment: 6223497 INFO @ Wed, 28 Jun 2017 04:56:25: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:56:25: #1 finished! INFO @ Wed, 28 Jun 2017 04:56:25: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:56:25: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:56:26: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:56:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:56:26: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 04:56:26: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:56:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:56:26: Process for pairing-model is terminated! cat: SRX1166300.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX1166300.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1166300.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1166300.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1166300.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1166300.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1166300.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1166300.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。