Job ID = 2009725 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,361,867 reads read : 17,361,867 reads written : 17,361,867 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:08 17361867 reads; of these: 17361867 (100.00%) were unpaired; of these: 3011755 (17.35%) aligned 0 times 11952085 (68.84%) aligned exactly 1 time 2398027 (13.81%) aligned >1 times 82.65% overall alignment rate Time searching: 00:03:08 Overall time: 00:03:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5187623 / 14350112 = 0.3615 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:51:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:51:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:51:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:51:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:51:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:51:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:51:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:51:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:51:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:51:24: 1000000 INFO @ Fri, 05 Jul 2019 19:51:25: 1000000 INFO @ Fri, 05 Jul 2019 19:51:26: 1000000 INFO @ Fri, 05 Jul 2019 19:51:33: 2000000 INFO @ Fri, 05 Jul 2019 19:51:36: 2000000 INFO @ Fri, 05 Jul 2019 19:51:36: 2000000 INFO @ Fri, 05 Jul 2019 19:51:42: 3000000 INFO @ Fri, 05 Jul 2019 19:51:43: 3000000 INFO @ Fri, 05 Jul 2019 19:51:44: 3000000 INFO @ Fri, 05 Jul 2019 19:51:50: 4000000 INFO @ Fri, 05 Jul 2019 19:51:50: 4000000 INFO @ Fri, 05 Jul 2019 19:51:51: 4000000 INFO @ Fri, 05 Jul 2019 19:51:57: 5000000 INFO @ Fri, 05 Jul 2019 19:51:58: 5000000 INFO @ Fri, 05 Jul 2019 19:51:58: 5000000 INFO @ Fri, 05 Jul 2019 19:52:04: 6000000 INFO @ Fri, 05 Jul 2019 19:52:05: 6000000 INFO @ Fri, 05 Jul 2019 19:52:07: 6000000 INFO @ Fri, 05 Jul 2019 19:52:11: 7000000 INFO @ Fri, 05 Jul 2019 19:52:13: 7000000 INFO @ Fri, 05 Jul 2019 19:52:15: 7000000 INFO @ Fri, 05 Jul 2019 19:52:18: 8000000 INFO @ Fri, 05 Jul 2019 19:52:20: 8000000 INFO @ Fri, 05 Jul 2019 19:52:24: 8000000 INFO @ Fri, 05 Jul 2019 19:52:24: 9000000 INFO @ Fri, 05 Jul 2019 19:52:26: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 19:52:26: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 19:52:26: #1 total tags in treatment: 9162489 INFO @ Fri, 05 Jul 2019 19:52:26: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:52:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:52:26: #1 tags after filtering in treatment: 9162489 INFO @ Fri, 05 Jul 2019 19:52:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:52:26: #1 finished! INFO @ Fri, 05 Jul 2019 19:52:26: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:52:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:52:26: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:52:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:52:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:52:27: 9000000 INFO @ Fri, 05 Jul 2019 19:52:28: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 19:52:28: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 19:52:28: #1 total tags in treatment: 9162489 INFO @ Fri, 05 Jul 2019 19:52:28: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:52:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:52:28: #1 tags after filtering in treatment: 9162489 INFO @ Fri, 05 Jul 2019 19:52:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:52:28: #1 finished! INFO @ Fri, 05 Jul 2019 19:52:28: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:52:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:52:29: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:52:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:52:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.10_peaks.narrowPeak: No such file or directory INFO @ Fri, 05 Jul 2019 19:52:32: 9000000 INFO @ Fri, 05 Jul 2019 19:52:33: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 19:52:33: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 19:52:33: #1 total tags in treatment: 9162489 INFO @ Fri, 05 Jul 2019 19:52:33: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:52:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:52:33: #1 tags after filtering in treatment: 9162489 INFO @ Fri, 05 Jul 2019 19:52:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:52:33: #1 finished! INFO @ Fri, 05 Jul 2019 19:52:33: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:52:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:52:34: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:52:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:52:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 2222 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163840/SRX1163840.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。