Job ID = 2009722 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,259,339 reads read : 16,259,339 reads written : 16,259,339 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:39 16259339 reads; of these: 16259339 (100.00%) were unpaired; of these: 4018545 (24.72%) aligned 0 times 10059841 (61.87%) aligned exactly 1 time 2180953 (13.41%) aligned >1 times 75.28% overall alignment rate Time searching: 00:02:39 Overall time: 00:02:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4374054 / 12240794 = 0.3573 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:48:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:48:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:48:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:48:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:48:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:48:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:48:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:48:18: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:48:18: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:48:25: 1000000 INFO @ Fri, 05 Jul 2019 19:48:25: 1000000 INFO @ Fri, 05 Jul 2019 19:48:25: 1000000 INFO @ Fri, 05 Jul 2019 19:48:33: 2000000 INFO @ Fri, 05 Jul 2019 19:48:33: 2000000 INFO @ Fri, 05 Jul 2019 19:48:34: 2000000 INFO @ Fri, 05 Jul 2019 19:48:40: 3000000 INFO @ Fri, 05 Jul 2019 19:48:41: 3000000 INFO @ Fri, 05 Jul 2019 19:48:43: 3000000 INFO @ Fri, 05 Jul 2019 19:48:47: 4000000 INFO @ Fri, 05 Jul 2019 19:48:48: 4000000 INFO @ Fri, 05 Jul 2019 19:48:51: 4000000 INFO @ Fri, 05 Jul 2019 19:48:54: 5000000 INFO @ Fri, 05 Jul 2019 19:48:55: 5000000 INFO @ Fri, 05 Jul 2019 19:48:59: 5000000 INFO @ Fri, 05 Jul 2019 19:49:01: 6000000 INFO @ Fri, 05 Jul 2019 19:49:02: 6000000 INFO @ Fri, 05 Jul 2019 19:49:07: 6000000 INFO @ Fri, 05 Jul 2019 19:49:08: 7000000 INFO @ Fri, 05 Jul 2019 19:49:09: 7000000 INFO @ Fri, 05 Jul 2019 19:49:15: 7000000 INFO @ Fri, 05 Jul 2019 19:49:20: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 19:49:20: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 19:49:22: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 19:49:22: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 19:49:22: #1 total tags in treatment: 7866740 INFO @ Fri, 05 Jul 2019 19:49:22: #1 total tags in treatment: 7866740 INFO @ Fri, 05 Jul 2019 19:49:22: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:49:22: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:49:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:49:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:49:23: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 19:49:23: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 19:49:23: #1 total tags in treatment: 7866740 INFO @ Fri, 05 Jul 2019 19:49:23: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:49:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:49:23: #1 tags after filtering in treatment: 7866740 INFO @ Fri, 05 Jul 2019 19:49:23: #1 tags after filtering in treatment: 7866740 INFO @ Fri, 05 Jul 2019 19:49:23: #1 tags after filtering in treatment: 7866740 INFO @ Fri, 05 Jul 2019 19:49:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:49:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:49:26: #1 finished! INFO @ Fri, 05 Jul 2019 19:49:26: #1 finished! INFO @ Fri, 05 Jul 2019 19:49:26: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:49:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:49:26: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:49:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:49:26: #1 finished! INFO @ Fri, 05 Jul 2019 19:49:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:49:26: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:49:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:49:27: #2 number of paired peaks: 0 INFO @ Fri, 05 Jul 2019 19:49:27: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:49:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:49:28: Process for pairing-model is terminated! WARNING @ Fri, 05 Jul 2019 19:49:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:49:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.10_peaks.narrowPeakcut: /home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.20_peaks.narrowPeak: No such file or directory : No such file or directory pass1 - making usageList (0 chroms): 2 millis pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.10_model.r’: No such file or directoryrm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.10_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:49:29: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:49:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:49:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163838/SRX1163838.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。