Job ID = 2009718 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,619,851 reads read : 16,619,851 reads written : 16,619,851 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:31 16619851 reads; of these: 16619851 (100.00%) were unpaired; of these: 5163153 (31.07%) aligned 0 times 9608006 (57.81%) aligned exactly 1 time 1848692 (11.12%) aligned >1 times 68.93% overall alignment rate Time searching: 00:02:31 Overall time: 00:02:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4019772 / 11456698 = 0.3509 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:50:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:50:32: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:50:32: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:50:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:50:33: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:50:33: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:50:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:50:34: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:50:34: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:50:39: 1000000 INFO @ Fri, 05 Jul 2019 19:50:40: 1000000 INFO @ Fri, 05 Jul 2019 19:50:41: 1000000 INFO @ Fri, 05 Jul 2019 19:50:46: 2000000 INFO @ Fri, 05 Jul 2019 19:50:47: 2000000 INFO @ Fri, 05 Jul 2019 19:50:48: 2000000 INFO @ Fri, 05 Jul 2019 19:50:52: 3000000 INFO @ Fri, 05 Jul 2019 19:50:54: 3000000 INFO @ Fri, 05 Jul 2019 19:50:54: 3000000 INFO @ Fri, 05 Jul 2019 19:50:58: 4000000 INFO @ Fri, 05 Jul 2019 19:51:00: 4000000 INFO @ Fri, 05 Jul 2019 19:51:01: 4000000 INFO @ Fri, 05 Jul 2019 19:51:05: 5000000 INFO @ Fri, 05 Jul 2019 19:51:07: 5000000 INFO @ Fri, 05 Jul 2019 19:51:08: 5000000 INFO @ Fri, 05 Jul 2019 19:51:11: 6000000 INFO @ Fri, 05 Jul 2019 19:51:14: 6000000 INFO @ Fri, 05 Jul 2019 19:51:14: 6000000 INFO @ Fri, 05 Jul 2019 19:51:17: 7000000 INFO @ Fri, 05 Jul 2019 19:51:20: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 19:51:20: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 19:51:20: #1 total tags in treatment: 7436926 INFO @ Fri, 05 Jul 2019 19:51:20: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:51:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:51:20: #1 tags after filtering in treatment: 7436926 INFO @ Fri, 05 Jul 2019 19:51:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:51:20: #1 finished! INFO @ Fri, 05 Jul 2019 19:51:20: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:51:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:51:20: 7000000 INFO @ Fri, 05 Jul 2019 19:51:21: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:51:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:51:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:51:21: 7000000 INFO @ Fri, 05 Jul 2019 19:51:23: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 19:51:23: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 19:51:23: #1 total tags in treatment: 7436926 INFO @ Fri, 05 Jul 2019 19:51:23: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:51:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:51:24: #1 tags after filtering in treatment: 7436926 INFO @ Fri, 05 Jul 2019 19:51:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:51:24: #1 finished! INFO @ Fri, 05 Jul 2019 19:51:24: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:51:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:51:24: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:51:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:51:24: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:51:24: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 19:51:24: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 19:51:24: #1 total tags in treatment: 7436926 INFO @ Fri, 05 Jul 2019 19:51:24: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:51:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cut: /home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:51:24: #1 tags after filtering in treatment: 7436926 INFO @ Fri, 05 Jul 2019 19:51:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:51:24: #1 finished! INFO @ Fri, 05 Jul 2019 19:51:24: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:51:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:51:25: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:51:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:51:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1163834/SRX1163834.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。