Job ID = 9161858 sra ファイルのダウンロード中... Completed: 646154K bytes transferred in 8 seconds (597595K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 19606095 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1142483/SRR2155478.sra Written 19606095 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:09 19606095 reads; of these: 19606095 (100.00%) were unpaired; of these: 1746767 (8.91%) aligned 0 times 15484513 (78.98%) aligned exactly 1 time 2374815 (12.11%) aligned >1 times 91.09% overall alignment rate Time searching: 00:04:09 Overall time: 00:04:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 9184001 / 17859328 = 0.5142 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:57:04: # Command line: callpeak -t SRX1142483.bam -f BAM -g 12100000 -n SRX1142483.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1142483.10 # format = BAM # ChIP-seq file = ['SRX1142483.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:57:04: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:57:04: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:57:04: # Command line: callpeak -t SRX1142483.bam -f BAM -g 12100000 -n SRX1142483.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1142483.20 # format = BAM # ChIP-seq file = ['SRX1142483.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:57:04: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:57:04: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:57:04: # Command line: callpeak -t SRX1142483.bam -f BAM -g 12100000 -n SRX1142483.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1142483.05 # format = BAM # ChIP-seq file = ['SRX1142483.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:57:04: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:57:04: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:57:11: 1000000 INFO @ Wed, 28 Jun 2017 04:57:11: 1000000 INFO @ Wed, 28 Jun 2017 04:57:11: 1000000 INFO @ Wed, 28 Jun 2017 04:57:17: 2000000 INFO @ Wed, 28 Jun 2017 04:57:18: 2000000 INFO @ Wed, 28 Jun 2017 04:57:18: 2000000 INFO @ Wed, 28 Jun 2017 04:57:24: 3000000 INFO @ Wed, 28 Jun 2017 04:57:26: 3000000 INFO @ Wed, 28 Jun 2017 04:57:26: 3000000 INFO @ Wed, 28 Jun 2017 04:57:31: 4000000 INFO @ Wed, 28 Jun 2017 04:57:33: 4000000 INFO @ Wed, 28 Jun 2017 04:57:34: 4000000 INFO @ Wed, 28 Jun 2017 04:57:39: 5000000 INFO @ Wed, 28 Jun 2017 04:57:41: 5000000 INFO @ Wed, 28 Jun 2017 04:57:42: 5000000 INFO @ Wed, 28 Jun 2017 04:57:47: 6000000 INFO @ Wed, 28 Jun 2017 04:57:49: 6000000 INFO @ Wed, 28 Jun 2017 04:57:50: 6000000 INFO @ Wed, 28 Jun 2017 04:57:55: 7000000 INFO @ Wed, 28 Jun 2017 04:57:57: 7000000 INFO @ Wed, 28 Jun 2017 04:57:59: 7000000 INFO @ Wed, 28 Jun 2017 04:58:03: 8000000 INFO @ Wed, 28 Jun 2017 04:58:05: 8000000 INFO @ Wed, 28 Jun 2017 04:58:07: 8000000 INFO @ Wed, 28 Jun 2017 04:58:09: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 04:58:09: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 04:58:09: #1 total tags in treatment: 8675327 INFO @ Wed, 28 Jun 2017 04:58:09: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:58:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:58:09: #1 tags after filtering in treatment: 8675327 INFO @ Wed, 28 Jun 2017 04:58:09: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:58:09: #1 finished! INFO @ Wed, 28 Jun 2017 04:58:09: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:58:09: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:58:09: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:58:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:58:09: Process for pairing-model is terminated! cat: SRX1142483.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1142483.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1142483.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1142483.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:58:11: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 04:58:11: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 04:58:11: #1 total tags in treatment: 8675327 INFO @ Wed, 28 Jun 2017 04:58:11: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:58:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:58:11: #1 tags after filtering in treatment: 8675327 INFO @ Wed, 28 Jun 2017 04:58:11: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:58:11: #1 finished! INFO @ Wed, 28 Jun 2017 04:58:11: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:58:11: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:58:11: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:58:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:58:11: Process for pairing-model is terminated! cat: SRX1142483.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1142483.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1142483.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1142483.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:58:12: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 04:58:12: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 04:58:12: #1 total tags in treatment: 8675327 INFO @ Wed, 28 Jun 2017 04:58:12: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:58:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:58:13: #1 tags after filtering in treatment: 8675327 INFO @ Wed, 28 Jun 2017 04:58:13: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:58:13: #1 finished! INFO @ Wed, 28 Jun 2017 04:58:13: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:58:13: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:58:13: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:58:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:58:13: Process for pairing-model is terminated! cat: SRX1142483.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1142483.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1142483.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1142483.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。