Job ID = 9161841 sra ファイルのダウンロード中... Completed: 169502K bytes transferred in 4 seconds (294513K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 7637258 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1142464/SRR2155459.sra Written 7637258 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:24 7637258 reads; of these: 7637258 (100.00%) were unpaired; of these: 304323 (3.98%) aligned 0 times 5963568 (78.09%) aligned exactly 1 time 1369367 (17.93%) aligned >1 times 96.02% overall alignment rate Time searching: 00:01:24 Overall time: 00:01:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1923802 / 7332935 = 0.2624 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:48:24: # Command line: callpeak -t SRX1142464.bam -f BAM -g 12100000 -n SRX1142464.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1142464.05 # format = BAM # ChIP-seq file = ['SRX1142464.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:48:24: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:48:24: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:48:24: # Command line: callpeak -t SRX1142464.bam -f BAM -g 12100000 -n SRX1142464.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1142464.10 # format = BAM # ChIP-seq file = ['SRX1142464.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:48:24: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:48:24: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:48:24: # Command line: callpeak -t SRX1142464.bam -f BAM -g 12100000 -n SRX1142464.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1142464.20 # format = BAM # ChIP-seq file = ['SRX1142464.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:48:24: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:48:24: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:48:30: 1000000 INFO @ Wed, 28 Jun 2017 04:48:30: 1000000 INFO @ Wed, 28 Jun 2017 04:48:30: 1000000 INFO @ Wed, 28 Jun 2017 04:48:36: 2000000 INFO @ Wed, 28 Jun 2017 04:48:37: 2000000 INFO @ Wed, 28 Jun 2017 04:48:37: 2000000 INFO @ Wed, 28 Jun 2017 04:48:42: 3000000 INFO @ Wed, 28 Jun 2017 04:48:44: 3000000 INFO @ Wed, 28 Jun 2017 04:48:44: 3000000 INFO @ Wed, 28 Jun 2017 04:48:49: 4000000 INFO @ Wed, 28 Jun 2017 04:48:50: 4000000 INFO @ Wed, 28 Jun 2017 04:48:51: 4000000 INFO @ Wed, 28 Jun 2017 04:48:55: 5000000 INFO @ Wed, 28 Jun 2017 04:48:57: 5000000 INFO @ Wed, 28 Jun 2017 04:48:58: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 04:48:58: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 04:48:58: #1 total tags in treatment: 5409133 INFO @ Wed, 28 Jun 2017 04:48:58: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:48:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:48:58: #1 tags after filtering in treatment: 5409133 INFO @ Wed, 28 Jun 2017 04:48:58: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:48:58: #1 finished! INFO @ Wed, 28 Jun 2017 04:48:58: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:48:58: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:48:58: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:48:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:48:58: Process for pairing-model is terminated! cat: SRX1142464.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1142464.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1142464.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1142464.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:48:58: 5000000 INFO @ Wed, 28 Jun 2017 04:48:59: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 04:48:59: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 04:48:59: #1 total tags in treatment: 5409133 INFO @ Wed, 28 Jun 2017 04:48:59: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:48:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:48:59: #1 tags after filtering in treatment: 5409133 INFO @ Wed, 28 Jun 2017 04:48:59: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:48:59: #1 finished! INFO @ Wed, 28 Jun 2017 04:48:59: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:48:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:49:00: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:49:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:49:00: Process for pairing-model is terminated! cat: SRX1142464.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1142464.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1142464.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1142464.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:49:01: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 04:49:01: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 04:49:01: #1 total tags in treatment: 5409133 INFO @ Wed, 28 Jun 2017 04:49:01: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:49:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:49:01: #1 tags after filtering in treatment: 5409133 INFO @ Wed, 28 Jun 2017 04:49:01: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:49:01: #1 finished! INFO @ Wed, 28 Jun 2017 04:49:01: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:49:01: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:49:01: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:49:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:49:01: Process for pairing-model is terminated! cat: SRX1142464.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1142464.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1142464.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1142464.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。