Job ID = 14520324 SRX = SRX11361077 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 33084157 spots for SRR15050654/SRR15050654.sra Written 33084157 spots for SRR15050654/SRR15050654.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:29 33084157 reads; of these: 33084157 (100.00%) were unpaired; of these: 1252255 (3.79%) aligned 0 times 25907105 (78.31%) aligned exactly 1 time 5924797 (17.91%) aligned >1 times 96.21% overall alignment rate Time searching: 00:07:29 Overall time: 00:07:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 25770354 / 31831902 = 0.8096 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:14:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:14:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:14:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:14:36: 1000000 INFO @ Sat, 15 Jan 2022 19:14:43: 2000000 INFO @ Sat, 15 Jan 2022 19:14:49: 3000000 INFO @ Sat, 15 Jan 2022 19:14:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:15:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:15:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:15:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:15:01: 5000000 INFO @ Sat, 15 Jan 2022 19:15:06: 1000000 INFO @ Sat, 15 Jan 2022 19:15:07: 6000000 INFO @ Sat, 15 Jan 2022 19:15:08: #1 tag size is determined as 69 bps INFO @ Sat, 15 Jan 2022 19:15:08: #1 tag size = 69 INFO @ Sat, 15 Jan 2022 19:15:08: #1 total tags in treatment: 6061548 INFO @ Sat, 15 Jan 2022 19:15:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:15:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:15:08: #1 tags after filtering in treatment: 6061548 INFO @ Sat, 15 Jan 2022 19:15:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:15:08: #1 finished! INFO @ Sat, 15 Jan 2022 19:15:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:15:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:15:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:15:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:15:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:15:12: 2000000 INFO @ Sat, 15 Jan 2022 19:15:19: 3000000 INFO @ Sat, 15 Jan 2022 19:15:25: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:15:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:15:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:15:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:15:31: 5000000 INFO @ Sat, 15 Jan 2022 19:15:37: 1000000 INFO @ Sat, 15 Jan 2022 19:15:38: 6000000 INFO @ Sat, 15 Jan 2022 19:15:38: #1 tag size is determined as 69 bps INFO @ Sat, 15 Jan 2022 19:15:38: #1 tag size = 69 INFO @ Sat, 15 Jan 2022 19:15:38: #1 total tags in treatment: 6061548 INFO @ Sat, 15 Jan 2022 19:15:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:15:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:15:39: #1 tags after filtering in treatment: 6061548 INFO @ Sat, 15 Jan 2022 19:15:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:15:39: #1 finished! INFO @ Sat, 15 Jan 2022 19:15:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:15:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:15:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:15:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:15:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:15:43: 2000000 INFO @ Sat, 15 Jan 2022 19:15:50: 3000000 INFO @ Sat, 15 Jan 2022 19:15:56: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:16:02: 5000000 INFO @ Sat, 15 Jan 2022 19:16:09: 6000000 INFO @ Sat, 15 Jan 2022 19:16:09: #1 tag size is determined as 69 bps INFO @ Sat, 15 Jan 2022 19:16:09: #1 tag size = 69 INFO @ Sat, 15 Jan 2022 19:16:09: #1 total tags in treatment: 6061548 INFO @ Sat, 15 Jan 2022 19:16:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:16:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:16:09: #1 tags after filtering in treatment: 6061548 INFO @ Sat, 15 Jan 2022 19:16:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:16:09: #1 finished! INFO @ Sat, 15 Jan 2022 19:16:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:16:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:16:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:16:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:16:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361077/SRX11361077.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。