Job ID = 14520302 SRX = SRX11361069 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 36052113 spots for SRR15050646/SRR15050646.sra Written 36052113 spots for SRR15050646/SRR15050646.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:58 36052113 reads; of these: 36052113 (100.00%) were unpaired; of these: 1459259 (4.05%) aligned 0 times 28069473 (77.86%) aligned exactly 1 time 6523381 (18.09%) aligned >1 times 95.95% overall alignment rate Time searching: 00:07:58 Overall time: 00:07:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 27888410 / 34592854 = 0.8062 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:12:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:12:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:12:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:12:22: 1000000 INFO @ Sat, 15 Jan 2022 19:12:29: 2000000 INFO @ Sat, 15 Jan 2022 19:12:36: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:12:43: 4000000 INFO @ Sat, 15 Jan 2022 19:12:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:12:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:12:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:12:51: 5000000 INFO @ Sat, 15 Jan 2022 19:12:52: 1000000 INFO @ Sat, 15 Jan 2022 19:12:59: 6000000 INFO @ Sat, 15 Jan 2022 19:13:00: 2000000 INFO @ Sat, 15 Jan 2022 19:13:04: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 19:13:04: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 19:13:04: #1 total tags in treatment: 6704444 INFO @ Sat, 15 Jan 2022 19:13:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:13:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:13:05: #1 tags after filtering in treatment: 6704444 INFO @ Sat, 15 Jan 2022 19:13:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:13:05: #1 finished! INFO @ Sat, 15 Jan 2022 19:13:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:13:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:13:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:13:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:13:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:13:08: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:13:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:13:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:13:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:13:15: 4000000 INFO @ Sat, 15 Jan 2022 19:13:22: 1000000 INFO @ Sat, 15 Jan 2022 19:13:23: 5000000 INFO @ Sat, 15 Jan 2022 19:13:30: 2000000 INFO @ Sat, 15 Jan 2022 19:13:31: 6000000 INFO @ Sat, 15 Jan 2022 19:13:36: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 19:13:36: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 19:13:36: #1 total tags in treatment: 6704444 INFO @ Sat, 15 Jan 2022 19:13:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:13:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:13:36: #1 tags after filtering in treatment: 6704444 INFO @ Sat, 15 Jan 2022 19:13:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:13:36: #1 finished! INFO @ Sat, 15 Jan 2022 19:13:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:13:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:13:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:13:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:13:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:13:38: 3000000 INFO @ Sat, 15 Jan 2022 19:13:45: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:13:53: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:14:00: 6000000 INFO @ Sat, 15 Jan 2022 19:14:05: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 19:14:05: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 19:14:05: #1 total tags in treatment: 6704444 INFO @ Sat, 15 Jan 2022 19:14:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:14:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:14:05: #1 tags after filtering in treatment: 6704444 INFO @ Sat, 15 Jan 2022 19:14:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:14:05: #1 finished! INFO @ Sat, 15 Jan 2022 19:14:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:14:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:14:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:14:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:14:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361069/SRX11361069.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling