Job ID = 14520301 SRX = SRX11361068 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25237906 spots for SRR15050645/SRR15050645.sra Written 25237906 spots for SRR15050645/SRR15050645.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:34 25237906 reads; of these: 25237906 (100.00%) were unpaired; of these: 1009191 (4.00%) aligned 0 times 19618680 (77.73%) aligned exactly 1 time 4610035 (18.27%) aligned >1 times 96.00% overall alignment rate Time searching: 00:04:34 Overall time: 00:04:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 20579966 / 24228715 = 0.8494 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:04:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:04:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:04:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:04:54: 1000000 INFO @ Sat, 15 Jan 2022 19:05:00: 2000000 INFO @ Sat, 15 Jan 2022 19:05:05: 3000000 INFO @ Sat, 15 Jan 2022 19:05:09: #1 tag size is determined as 69 bps INFO @ Sat, 15 Jan 2022 19:05:09: #1 tag size = 69 INFO @ Sat, 15 Jan 2022 19:05:09: #1 total tags in treatment: 3648749 INFO @ Sat, 15 Jan 2022 19:05:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:05:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:05:09: #1 tags after filtering in treatment: 3648749 INFO @ Sat, 15 Jan 2022 19:05:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:05:09: #1 finished! INFO @ Sat, 15 Jan 2022 19:05:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:05:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:05:09: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 19:05:09: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:05:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:05:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:05:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:05:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:24: 1000000 INFO @ Sat, 15 Jan 2022 19:05:30: 2000000 INFO @ Sat, 15 Jan 2022 19:05:35: 3000000 INFO @ Sat, 15 Jan 2022 19:05:39: #1 tag size is determined as 69 bps INFO @ Sat, 15 Jan 2022 19:05:39: #1 tag size = 69 INFO @ Sat, 15 Jan 2022 19:05:39: #1 total tags in treatment: 3648749 INFO @ Sat, 15 Jan 2022 19:05:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:05:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:05:39: #1 tags after filtering in treatment: 3648749 INFO @ Sat, 15 Jan 2022 19:05:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:05:39: #1 finished! INFO @ Sat, 15 Jan 2022 19:05:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:05:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:05:39: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 19:05:39: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:05:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:05:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:05:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:05:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:55: 1000000 INFO @ Sat, 15 Jan 2022 19:06:00: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:06:06: 3000000 INFO @ Sat, 15 Jan 2022 19:06:09: #1 tag size is determined as 69 bps INFO @ Sat, 15 Jan 2022 19:06:09: #1 tag size = 69 INFO @ Sat, 15 Jan 2022 19:06:09: #1 total tags in treatment: 3648749 INFO @ Sat, 15 Jan 2022 19:06:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:06:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:06:09: #1 tags after filtering in treatment: 3648749 INFO @ Sat, 15 Jan 2022 19:06:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:06:09: #1 finished! INFO @ Sat, 15 Jan 2022 19:06:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:06:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:06:09: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 19:06:09: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:06:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361068/SRX11361068.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。