Job ID = 14520298 SRX = SRX11361065 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 32549371 spots for SRR15050642/SRR15050642.sra Written 32549371 spots for SRR15050642/SRR15050642.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:09 32549371 reads; of these: 32549371 (100.00%) were unpaired; of these: 1137493 (3.49%) aligned 0 times 25474533 (78.26%) aligned exactly 1 time 5937345 (18.24%) aligned >1 times 96.51% overall alignment rate Time searching: 00:07:09 Overall time: 00:07:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 26267947 / 31411878 = 0.8362 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:10:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:10:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:10:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:10:14: 1000000 INFO @ Sat, 15 Jan 2022 19:10:21: 2000000 INFO @ Sat, 15 Jan 2022 19:10:27: 3000000 INFO @ Sat, 15 Jan 2022 19:10:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:10:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:10:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:10:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:10:40: 5000000 INFO @ Sat, 15 Jan 2022 19:10:41: #1 tag size is determined as 72 bps INFO @ Sat, 15 Jan 2022 19:10:41: #1 tag size = 72 INFO @ Sat, 15 Jan 2022 19:10:41: #1 total tags in treatment: 5143931 INFO @ Sat, 15 Jan 2022 19:10:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:10:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:10:41: #1 tags after filtering in treatment: 5143931 INFO @ Sat, 15 Jan 2022 19:10:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:10:41: #1 finished! INFO @ Sat, 15 Jan 2022 19:10:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:10:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:10:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:10:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:10:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:10:45: 1000000 INFO @ Sat, 15 Jan 2022 19:10:51: 2000000 INFO @ Sat, 15 Jan 2022 19:10:58: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:11:05: 4000000 INFO @ Sat, 15 Jan 2022 19:11:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:11:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:11:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:11:12: 5000000 INFO @ Sat, 15 Jan 2022 19:11:13: #1 tag size is determined as 72 bps INFO @ Sat, 15 Jan 2022 19:11:13: #1 tag size = 72 INFO @ Sat, 15 Jan 2022 19:11:13: #1 total tags in treatment: 5143931 INFO @ Sat, 15 Jan 2022 19:11:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:11:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:11:13: #1 tags after filtering in treatment: 5143931 INFO @ Sat, 15 Jan 2022 19:11:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:11:13: #1 finished! INFO @ Sat, 15 Jan 2022 19:11:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:11:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:11:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:11:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:11:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:11:15: 1000000 INFO @ Sat, 15 Jan 2022 19:11:21: 2000000 INFO @ Sat, 15 Jan 2022 19:11:28: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:11:34: 4000000 INFO @ Sat, 15 Jan 2022 19:11:41: 5000000 INFO @ Sat, 15 Jan 2022 19:11:42: #1 tag size is determined as 72 bps INFO @ Sat, 15 Jan 2022 19:11:42: #1 tag size = 72 INFO @ Sat, 15 Jan 2022 19:11:42: #1 total tags in treatment: 5143931 INFO @ Sat, 15 Jan 2022 19:11:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:11:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:11:42: #1 tags after filtering in treatment: 5143931 INFO @ Sat, 15 Jan 2022 19:11:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:11:42: #1 finished! INFO @ Sat, 15 Jan 2022 19:11:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:11:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:11:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:11:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:11:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361065/SRX11361065.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。