Job ID = 14520297 SRX = SRX11361064 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 24967777 spots for SRR15050641/SRR15050641.sra Written 24967777 spots for SRR15050641/SRR15050641.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:12 24967777 reads; of these: 24967777 (100.00%) were unpaired; of these: 1178723 (4.72%) aligned 0 times 19131381 (76.62%) aligned exactly 1 time 4657673 (18.65%) aligned >1 times 95.28% overall alignment rate Time searching: 00:06:12 Overall time: 00:06:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 19597509 / 23789054 = 0.8238 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:07:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:07:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:07:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:07:48: 1000000 INFO @ Sat, 15 Jan 2022 19:07:56: 2000000 INFO @ Sat, 15 Jan 2022 19:08:03: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:08:10: 4000000 INFO @ Sat, 15 Jan 2022 19:08:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:08:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:08:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:08:12: #1 tag size is determined as 70 bps INFO @ Sat, 15 Jan 2022 19:08:12: #1 tag size = 70 INFO @ Sat, 15 Jan 2022 19:08:12: #1 total tags in treatment: 4191545 INFO @ Sat, 15 Jan 2022 19:08:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:08:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:08:12: #1 tags after filtering in treatment: 4191545 INFO @ Sat, 15 Jan 2022 19:08:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:08:12: #1 finished! INFO @ Sat, 15 Jan 2022 19:08:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:08:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:08:12: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 19:08:12: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:08:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:08:18: 1000000 INFO @ Sat, 15 Jan 2022 19:08:26: 2000000 INFO @ Sat, 15 Jan 2022 19:08:33: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:08:40: 4000000 INFO @ Sat, 15 Jan 2022 19:08:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:08:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:08:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:08:42: #1 tag size is determined as 70 bps INFO @ Sat, 15 Jan 2022 19:08:42: #1 tag size = 70 INFO @ Sat, 15 Jan 2022 19:08:42: #1 total tags in treatment: 4191545 INFO @ Sat, 15 Jan 2022 19:08:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:08:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:08:42: #1 tags after filtering in treatment: 4191545 INFO @ Sat, 15 Jan 2022 19:08:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:08:42: #1 finished! INFO @ Sat, 15 Jan 2022 19:08:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:08:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:08:42: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 19:08:42: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:08:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:08:48: 1000000 INFO @ Sat, 15 Jan 2022 19:08:55: 2000000 INFO @ Sat, 15 Jan 2022 19:09:03: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:09:10: 4000000 INFO @ Sat, 15 Jan 2022 19:09:11: #1 tag size is determined as 70 bps INFO @ Sat, 15 Jan 2022 19:09:11: #1 tag size = 70 INFO @ Sat, 15 Jan 2022 19:09:11: #1 total tags in treatment: 4191545 INFO @ Sat, 15 Jan 2022 19:09:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:09:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:09:11: #1 tags after filtering in treatment: 4191545 INFO @ Sat, 15 Jan 2022 19:09:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:09:11: #1 finished! INFO @ Sat, 15 Jan 2022 19:09:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:09:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:09:12: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 19:09:12: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:09:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11361064/SRX11361064.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。