Job ID = 2009712 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 34,800,275 reads read : 34,800,275 reads written : 34,800,275 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:19 34800275 reads; of these: 34800275 (100.00%) were unpaired; of these: 5601674 (16.10%) aligned 0 times 24573209 (70.61%) aligned exactly 1 time 4625392 (13.29%) aligned >1 times 83.90% overall alignment rate Time searching: 00:12:19 Overall time: 00:12:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 16864673 / 29198601 = 0.5776 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:13:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:13:13: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:13:13: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:13:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:13:14: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:13:14: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:13:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:13:14: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:13:14: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:13:20: 1000000 INFO @ Fri, 05 Jul 2019 20:13:21: 1000000 INFO @ Fri, 05 Jul 2019 20:13:21: 1000000 INFO @ Fri, 05 Jul 2019 20:13:27: 2000000 INFO @ Fri, 05 Jul 2019 20:13:27: 2000000 INFO @ Fri, 05 Jul 2019 20:13:28: 2000000 INFO @ Fri, 05 Jul 2019 20:13:33: 3000000 INFO @ Fri, 05 Jul 2019 20:13:34: 3000000 INFO @ Fri, 05 Jul 2019 20:13:34: 3000000 INFO @ Fri, 05 Jul 2019 20:13:39: 4000000 INFO @ Fri, 05 Jul 2019 20:13:40: 4000000 INFO @ Fri, 05 Jul 2019 20:13:41: 4000000 INFO @ Fri, 05 Jul 2019 20:13:45: 5000000 INFO @ Fri, 05 Jul 2019 20:13:47: 5000000 INFO @ Fri, 05 Jul 2019 20:13:47: 5000000 INFO @ Fri, 05 Jul 2019 20:13:51: 6000000 INFO @ Fri, 05 Jul 2019 20:13:53: 6000000 INFO @ Fri, 05 Jul 2019 20:13:54: 6000000 INFO @ Fri, 05 Jul 2019 20:13:57: 7000000 INFO @ Fri, 05 Jul 2019 20:14:00: 7000000 INFO @ Fri, 05 Jul 2019 20:14:00: 7000000 INFO @ Fri, 05 Jul 2019 20:14:04: 8000000 INFO @ Fri, 05 Jul 2019 20:14:07: 8000000 INFO @ Fri, 05 Jul 2019 20:14:08: 8000000 INFO @ Fri, 05 Jul 2019 20:14:10: 9000000 INFO @ Fri, 05 Jul 2019 20:14:14: 9000000 INFO @ Fri, 05 Jul 2019 20:14:15: 9000000 INFO @ Fri, 05 Jul 2019 20:14:16: 10000000 INFO @ Fri, 05 Jul 2019 20:14:22: 10000000 INFO @ Fri, 05 Jul 2019 20:14:22: 10000000 INFO @ Fri, 05 Jul 2019 20:14:23: 11000000 INFO @ Fri, 05 Jul 2019 20:14:28: 11000000 INFO @ Fri, 05 Jul 2019 20:14:29: 11000000 INFO @ Fri, 05 Jul 2019 20:14:29: 12000000 INFO @ Fri, 05 Jul 2019 20:14:31: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 20:14:31: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 20:14:31: #1 total tags in treatment: 12333928 INFO @ Fri, 05 Jul 2019 20:14:31: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:14:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:14:31: #1 tags after filtering in treatment: 12333928 INFO @ Fri, 05 Jul 2019 20:14:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:14:31: #1 finished! INFO @ Fri, 05 Jul 2019 20:14:31: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:14:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:14:32: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:14:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:14:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:14:35: 12000000 INFO @ Fri, 05 Jul 2019 20:14:36: 12000000 INFO @ Fri, 05 Jul 2019 20:14:38: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 20:14:38: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 20:14:38: #1 total tags in treatment: 12333928 INFO @ Fri, 05 Jul 2019 20:14:38: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:14:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:14:38: #1 tags after filtering in treatment: 12333928 INFO @ Fri, 05 Jul 2019 20:14:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:14:38: #1 finished! INFO @ Fri, 05 Jul 2019 20:14:38: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:14:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:14:38: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 20:14:38: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 20:14:38: #1 total tags in treatment: 12333928 INFO @ Fri, 05 Jul 2019 20:14:38: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:14:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:14:38: #1 tags after filtering in treatment: 12333928 INFO @ Fri, 05 Jul 2019 20:14:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:14:38: #1 finished! INFO @ Fri, 05 Jul 2019 20:14:38: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:14:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:14:39: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:14:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:14:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:14:39: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:14:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:14:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1135892/SRX1135892.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。