Job ID = 14521807 SRX = SRX11320612 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 35385982 spots for SRR15008448/SRR15008448.sra Written 35385982 spots for SRR15008448/SRR15008448.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:34 35385982 reads; of these: 35385982 (100.00%) were unpaired; of these: 7010251 (19.81%) aligned 0 times 25965755 (73.38%) aligned exactly 1 time 2409976 (6.81%) aligned >1 times 80.19% overall alignment rate Time searching: 00:04:34 Overall time: 00:04:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 16412712 / 28375731 = 0.5784 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:47:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:47:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:47:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:47:39: 1000000 INFO @ Sat, 15 Jan 2022 21:47:44: 2000000 INFO @ Sat, 15 Jan 2022 21:47:49: 3000000 INFO @ Sat, 15 Jan 2022 21:47:55: 4000000 INFO @ Sat, 15 Jan 2022 21:48:00: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:48:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:48:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:48:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:48:05: 6000000 INFO @ Sat, 15 Jan 2022 21:48:10: 1000000 INFO @ Sat, 15 Jan 2022 21:48:12: 7000000 INFO @ Sat, 15 Jan 2022 21:48:17: 2000000 INFO @ Sat, 15 Jan 2022 21:48:18: 8000000 INFO @ Sat, 15 Jan 2022 21:48:24: 3000000 INFO @ Sat, 15 Jan 2022 21:48:25: 9000000 INFO @ Sat, 15 Jan 2022 21:48:31: 4000000 INFO @ Sat, 15 Jan 2022 21:48:31: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:48:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:48:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:48:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:48:37: 11000000 INFO @ Sat, 15 Jan 2022 21:48:38: 5000000 INFO @ Sat, 15 Jan 2022 21:48:40: 1000000 INFO @ Sat, 15 Jan 2022 21:48:43: #1 tag size is determined as 49 bps INFO @ Sat, 15 Jan 2022 21:48:43: #1 tag size = 49 INFO @ Sat, 15 Jan 2022 21:48:43: #1 total tags in treatment: 11963019 INFO @ Sat, 15 Jan 2022 21:48:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:48:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:48:44: #1 tags after filtering in treatment: 11963019 INFO @ Sat, 15 Jan 2022 21:48:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:48:44: #1 finished! INFO @ Sat, 15 Jan 2022 21:48:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:48:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:48:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:48:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:48:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:48:45: 6000000 INFO @ Sat, 15 Jan 2022 21:48:48: 2000000 INFO @ Sat, 15 Jan 2022 21:48:52: 7000000 INFO @ Sat, 15 Jan 2022 21:48:55: 3000000 INFO @ Sat, 15 Jan 2022 21:48:59: 8000000 INFO @ Sat, 15 Jan 2022 21:49:02: 4000000 INFO @ Sat, 15 Jan 2022 21:49:06: 9000000 INFO @ Sat, 15 Jan 2022 21:49:09: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:49:12: 10000000 INFO @ Sat, 15 Jan 2022 21:49:16: 6000000 INFO @ Sat, 15 Jan 2022 21:49:19: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:49:23: 7000000 INFO @ Sat, 15 Jan 2022 21:49:26: #1 tag size is determined as 49 bps INFO @ Sat, 15 Jan 2022 21:49:26: #1 tag size = 49 INFO @ Sat, 15 Jan 2022 21:49:26: #1 total tags in treatment: 11963019 INFO @ Sat, 15 Jan 2022 21:49:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:49:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:49:26: #1 tags after filtering in treatment: 11963019 INFO @ Sat, 15 Jan 2022 21:49:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:49:26: #1 finished! INFO @ Sat, 15 Jan 2022 21:49:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:49:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:49:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:49:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:49:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:49:30: 8000000 INFO @ Sat, 15 Jan 2022 21:49:36: 9000000 INFO @ Sat, 15 Jan 2022 21:49:42: 10000000 INFO @ Sat, 15 Jan 2022 21:49:49: 11000000 INFO @ Sat, 15 Jan 2022 21:49:55: #1 tag size is determined as 49 bps INFO @ Sat, 15 Jan 2022 21:49:55: #1 tag size = 49 INFO @ Sat, 15 Jan 2022 21:49:55: #1 total tags in treatment: 11963019 INFO @ Sat, 15 Jan 2022 21:49:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:49:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:49:55: #1 tags after filtering in treatment: 11963019 INFO @ Sat, 15 Jan 2022 21:49:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:49:55: #1 finished! INFO @ Sat, 15 Jan 2022 21:49:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:49:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:49:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:49:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:49:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320612/SRX11320612.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling