Job ID = 14521805 SRX = SRX11320610 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 32925281 spots for SRR15008446/SRR15008446.sra Written 32925281 spots for SRR15008446/SRR15008446.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:23 32925281 reads; of these: 32925281 (100.00%) were unpaired; of these: 6829831 (20.74%) aligned 0 times 23279498 (70.70%) aligned exactly 1 time 2815952 (8.55%) aligned >1 times 79.26% overall alignment rate Time searching: 00:06:23 Overall time: 00:06:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 15149509 / 26095450 = 0.5805 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:52:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:52:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:52:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:52:15: 1000000 INFO @ Sat, 15 Jan 2022 21:52:25: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:52:35: 3000000 INFO @ Sat, 15 Jan 2022 21:52:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:52:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:52:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:52:45: 4000000 INFO @ Sat, 15 Jan 2022 21:52:47: 1000000 INFO @ Sat, 15 Jan 2022 21:52:55: 5000000 INFO @ Sat, 15 Jan 2022 21:52:58: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:53:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:53:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:53:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:53:05: 6000000 INFO @ Sat, 15 Jan 2022 21:53:09: 3000000 INFO @ Sat, 15 Jan 2022 21:53:16: 1000000 INFO @ Sat, 15 Jan 2022 21:53:16: 7000000 INFO @ Sat, 15 Jan 2022 21:53:21: 4000000 INFO @ Sat, 15 Jan 2022 21:53:26: 8000000 INFO @ Sat, 15 Jan 2022 21:53:27: 2000000 INFO @ Sat, 15 Jan 2022 21:53:32: 5000000 INFO @ Sat, 15 Jan 2022 21:53:37: 9000000 INFO @ Sat, 15 Jan 2022 21:53:37: 3000000 INFO @ Sat, 15 Jan 2022 21:53:43: 6000000 INFO @ Sat, 15 Jan 2022 21:53:48: 10000000 INFO @ Sat, 15 Jan 2022 21:53:48: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:53:55: 7000000 INFO @ Sat, 15 Jan 2022 21:53:58: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:53:58: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:53:58: #1 total tags in treatment: 10945941 INFO @ Sat, 15 Jan 2022 21:53:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:53:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:53:58: 5000000 INFO @ Sat, 15 Jan 2022 21:53:58: #1 tags after filtering in treatment: 10945941 INFO @ Sat, 15 Jan 2022 21:53:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:53:58: #1 finished! INFO @ Sat, 15 Jan 2022 21:53:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:53:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:53:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:53:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:53:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:54:07: 8000000 INFO @ Sat, 15 Jan 2022 21:54:08: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:54:18: 7000000 INFO @ Sat, 15 Jan 2022 21:54:19: 9000000 INFO @ Sat, 15 Jan 2022 21:54:28: 8000000 INFO @ Sat, 15 Jan 2022 21:54:31: 10000000 INFO @ Sat, 15 Jan 2022 21:54:38: 9000000 INFO @ Sat, 15 Jan 2022 21:54:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:54:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:54:43: #1 total tags in treatment: 10945941 INFO @ Sat, 15 Jan 2022 21:54:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:54:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:54:43: #1 tags after filtering in treatment: 10945941 INFO @ Sat, 15 Jan 2022 21:54:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:54:43: #1 finished! INFO @ Sat, 15 Jan 2022 21:54:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:54:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:54:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:54:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:54:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:54:48: 10000000 INFO @ Sat, 15 Jan 2022 21:54:57: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:54:57: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:54:57: #1 total tags in treatment: 10945941 INFO @ Sat, 15 Jan 2022 21:54:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:54:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:54:57: #1 tags after filtering in treatment: 10945941 INFO @ Sat, 15 Jan 2022 21:54:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:54:57: #1 finished! INFO @ Sat, 15 Jan 2022 21:54:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:54:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:54:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:54:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:54:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320610/SRX11320610.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling