Job ID = 14521779 SRX = SRX11320609 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 35130692 spots for SRR15008445/SRR15008445.sra Written 35130692 spots for SRR15008445/SRR15008445.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:29 35130692 reads; of these: 35130692 (100.00%) were unpaired; of these: 6710368 (19.10%) aligned 0 times 26067977 (74.20%) aligned exactly 1 time 2352347 (6.70%) aligned >1 times 80.90% overall alignment rate Time searching: 00:06:29 Overall time: 00:06:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 15542527 / 28420324 = 0.5469 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:49:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:49:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:49:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:49:54: 1000000 INFO @ Sat, 15 Jan 2022 21:50:01: 2000000 INFO @ Sat, 15 Jan 2022 21:50:08: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:50:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:50:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:50:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:50:15: 4000000 INFO @ Sat, 15 Jan 2022 21:50:22: 5000000 INFO @ Sat, 15 Jan 2022 21:50:23: 1000000 INFO @ Sat, 15 Jan 2022 21:50:30: 6000000 INFO @ Sat, 15 Jan 2022 21:50:31: 2000000 INFO @ Sat, 15 Jan 2022 21:50:37: 7000000 INFO @ Sat, 15 Jan 2022 21:50:39: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:50:44: 8000000 INFO @ Sat, 15 Jan 2022 21:50:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:50:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:50:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:50:47: 4000000 INFO @ Sat, 15 Jan 2022 21:50:52: 9000000 INFO @ Sat, 15 Jan 2022 21:50:54: 1000000 INFO @ Sat, 15 Jan 2022 21:50:56: 5000000 INFO @ Sat, 15 Jan 2022 21:51:00: 10000000 INFO @ Sat, 15 Jan 2022 21:51:02: 2000000 INFO @ Sat, 15 Jan 2022 21:51:04: 6000000 INFO @ Sat, 15 Jan 2022 21:51:07: 11000000 INFO @ Sat, 15 Jan 2022 21:51:11: 3000000 INFO @ Sat, 15 Jan 2022 21:51:13: 7000000 INFO @ Sat, 15 Jan 2022 21:51:15: 12000000 INFO @ Sat, 15 Jan 2022 21:51:20: 4000000 INFO @ Sat, 15 Jan 2022 21:51:22: #1 tag size is determined as 48 bps INFO @ Sat, 15 Jan 2022 21:51:22: #1 tag size = 48 INFO @ Sat, 15 Jan 2022 21:51:22: #1 total tags in treatment: 12877797 INFO @ Sat, 15 Jan 2022 21:51:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:51:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:51:22: 8000000 INFO @ Sat, 15 Jan 2022 21:51:22: #1 tags after filtering in treatment: 12877797 INFO @ Sat, 15 Jan 2022 21:51:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:51:22: #1 finished! INFO @ Sat, 15 Jan 2022 21:51:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:51:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:51:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:51:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:51:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:51:28: 5000000 INFO @ Sat, 15 Jan 2022 21:51:31: 9000000 INFO @ Sat, 15 Jan 2022 21:51:37: 6000000 INFO @ Sat, 15 Jan 2022 21:51:39: 10000000 INFO @ Sat, 15 Jan 2022 21:51:45: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:51:48: 11000000 INFO @ Sat, 15 Jan 2022 21:51:53: 8000000 INFO @ Sat, 15 Jan 2022 21:51:57: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:52:02: 9000000 INFO @ Sat, 15 Jan 2022 21:52:05: #1 tag size is determined as 48 bps INFO @ Sat, 15 Jan 2022 21:52:05: #1 tag size = 48 INFO @ Sat, 15 Jan 2022 21:52:05: #1 total tags in treatment: 12877797 INFO @ Sat, 15 Jan 2022 21:52:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:52:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:52:05: #1 tags after filtering in treatment: 12877797 INFO @ Sat, 15 Jan 2022 21:52:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:52:05: #1 finished! INFO @ Sat, 15 Jan 2022 21:52:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:52:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:52:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:52:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:52:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:52:10: 10000000 INFO @ Sat, 15 Jan 2022 21:52:18: 11000000 INFO @ Sat, 15 Jan 2022 21:52:27: 12000000 INFO @ Sat, 15 Jan 2022 21:52:34: #1 tag size is determined as 48 bps INFO @ Sat, 15 Jan 2022 21:52:34: #1 tag size = 48 INFO @ Sat, 15 Jan 2022 21:52:34: #1 total tags in treatment: 12877797 INFO @ Sat, 15 Jan 2022 21:52:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:52:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:52:34: #1 tags after filtering in treatment: 12877797 INFO @ Sat, 15 Jan 2022 21:52:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:52:34: #1 finished! INFO @ Sat, 15 Jan 2022 21:52:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:52:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:52:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:52:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:52:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320609/SRX11320609.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling