Job ID = 14521778 SRX = SRX11320608 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 32591849 spots for SRR15008444/SRR15008444.sra Written 32591849 spots for SRR15008444/SRR15008444.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:05 32591849 reads; of these: 32591849 (100.00%) were unpaired; of these: 5512315 (16.91%) aligned 0 times 24742279 (75.92%) aligned exactly 1 time 2337255 (7.17%) aligned >1 times 83.09% overall alignment rate Time searching: 00:05:05 Overall time: 00:05:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14494741 / 27079534 = 0.5353 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:47:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:47:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:47:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:47:40: 1000000 INFO @ Sat, 15 Jan 2022 21:47:45: 2000000 INFO @ Sat, 15 Jan 2022 21:47:50: 3000000 INFO @ Sat, 15 Jan 2022 21:47:55: 4000000 INFO @ Sat, 15 Jan 2022 21:48:00: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:48:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:48:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:48:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:48:06: 6000000 INFO @ Sat, 15 Jan 2022 21:48:10: 1000000 INFO @ Sat, 15 Jan 2022 21:48:12: 7000000 INFO @ Sat, 15 Jan 2022 21:48:16: 2000000 INFO @ Sat, 15 Jan 2022 21:48:17: 8000000 INFO @ Sat, 15 Jan 2022 21:48:22: 3000000 INFO @ Sat, 15 Jan 2022 21:48:23: 9000000 INFO @ Sat, 15 Jan 2022 21:48:27: 4000000 INFO @ Sat, 15 Jan 2022 21:48:29: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:48:33: 5000000 INFO @ Sat, 15 Jan 2022 21:48:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:48:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:48:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:48:35: 11000000 INFO @ Sat, 15 Jan 2022 21:48:40: 6000000 INFO @ Sat, 15 Jan 2022 21:48:41: 1000000 INFO @ Sat, 15 Jan 2022 21:48:41: 12000000 INFO @ Sat, 15 Jan 2022 21:48:45: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:48:45: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:48:45: #1 total tags in treatment: 12584793 INFO @ Sat, 15 Jan 2022 21:48:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:48:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:48:45: #1 tags after filtering in treatment: 12584793 INFO @ Sat, 15 Jan 2022 21:48:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:48:45: #1 finished! INFO @ Sat, 15 Jan 2022 21:48:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:48:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:48:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:48:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:48:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) INFO @ Sat, 15 Jan 2022 21:48:46: 7000000 rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:48:47: 2000000 INFO @ Sat, 15 Jan 2022 21:48:52: 8000000 INFO @ Sat, 15 Jan 2022 21:48:53: 3000000 INFO @ Sat, 15 Jan 2022 21:48:58: 9000000 INFO @ Sat, 15 Jan 2022 21:48:59: 4000000 INFO @ Sat, 15 Jan 2022 21:49:03: 10000000 INFO @ Sat, 15 Jan 2022 21:49:05: 5000000 INFO @ Sat, 15 Jan 2022 21:49:09: 11000000 INFO @ Sat, 15 Jan 2022 21:49:11: 6000000 INFO @ Sat, 15 Jan 2022 21:49:16: 12000000 INFO @ Sat, 15 Jan 2022 21:49:17: 7000000 INFO @ Sat, 15 Jan 2022 21:49:19: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:49:19: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:49:19: #1 total tags in treatment: 12584793 INFO @ Sat, 15 Jan 2022 21:49:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:49:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:49:19: #1 tags after filtering in treatment: 12584793 INFO @ Sat, 15 Jan 2022 21:49:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:49:19: #1 finished! INFO @ Sat, 15 Jan 2022 21:49:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:49:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:49:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:49:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:49:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:49:23: 8000000 INFO @ Sat, 15 Jan 2022 21:49:28: 9000000 INFO @ Sat, 15 Jan 2022 21:49:33: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:49:38: 11000000 INFO @ Sat, 15 Jan 2022 21:49:44: 12000000 INFO @ Sat, 15 Jan 2022 21:49:47: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:49:47: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:49:47: #1 total tags in treatment: 12584793 INFO @ Sat, 15 Jan 2022 21:49:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:49:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:49:47: #1 tags after filtering in treatment: 12584793 INFO @ Sat, 15 Jan 2022 21:49:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:49:47: #1 finished! INFO @ Sat, 15 Jan 2022 21:49:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:49:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:49:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:49:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:49:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320608/SRX11320608.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling