Job ID = 14521776 SRX = SRX11320606 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 35107341 spots for SRR15008441/SRR15008441.sra Written 35107341 spots for SRR15008441/SRR15008441.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:32 35107341 reads; of these: 35107341 (100.00%) were unpaired; of these: 7537954 (21.47%) aligned 0 times 24479140 (69.73%) aligned exactly 1 time 3090247 (8.80%) aligned >1 times 78.53% overall alignment rate Time searching: 00:04:32 Overall time: 00:04:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 16390359 / 27569387 = 0.5945 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:45:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:45:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:45:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:45:16: 1000000 INFO @ Sat, 15 Jan 2022 21:45:21: 2000000 INFO @ Sat, 15 Jan 2022 21:45:25: 3000000 INFO @ Sat, 15 Jan 2022 21:45:30: 4000000 INFO @ Sat, 15 Jan 2022 21:45:34: 5000000 INFO @ Sat, 15 Jan 2022 21:45:39: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:45:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:45:42: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:45:42: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:45:43: 7000000 INFO @ Sat, 15 Jan 2022 21:45:47: 1000000 INFO @ Sat, 15 Jan 2022 21:45:48: 8000000 INFO @ Sat, 15 Jan 2022 21:45:53: 2000000 INFO @ Sat, 15 Jan 2022 21:45:53: 9000000 INFO @ Sat, 15 Jan 2022 21:45:58: 10000000 INFO @ Sat, 15 Jan 2022 21:45:58: 3000000 INFO @ Sat, 15 Jan 2022 21:46:03: 11000000 INFO @ Sat, 15 Jan 2022 21:46:03: 4000000 INFO @ Sat, 15 Jan 2022 21:46:04: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:46:04: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:46:04: #1 total tags in treatment: 11179028 INFO @ Sat, 15 Jan 2022 21:46:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:46:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:46:04: #1 tags after filtering in treatment: 11179028 INFO @ Sat, 15 Jan 2022 21:46:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:46:04: #1 finished! INFO @ Sat, 15 Jan 2022 21:46:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:46:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:46:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:46:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:46:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:46:09: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:46:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:46:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:46:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:46:14: 6000000 INFO @ Sat, 15 Jan 2022 21:46:16: 1000000 INFO @ Sat, 15 Jan 2022 21:46:20: 7000000 INFO @ Sat, 15 Jan 2022 21:46:21: 2000000 INFO @ Sat, 15 Jan 2022 21:46:26: 8000000 INFO @ Sat, 15 Jan 2022 21:46:26: 3000000 INFO @ Sat, 15 Jan 2022 21:46:31: 9000000 INFO @ Sat, 15 Jan 2022 21:46:31: 4000000 INFO @ Sat, 15 Jan 2022 21:46:36: 5000000 INFO @ Sat, 15 Jan 2022 21:46:37: 10000000 INFO @ Sat, 15 Jan 2022 21:46:41: 6000000 INFO @ Sat, 15 Jan 2022 21:46:42: 11000000 INFO @ Sat, 15 Jan 2022 21:46:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:46:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:46:43: #1 total tags in treatment: 11179028 INFO @ Sat, 15 Jan 2022 21:46:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:46:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:46:43: #1 tags after filtering in treatment: 11179028 INFO @ Sat, 15 Jan 2022 21:46:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:46:43: #1 finished! INFO @ Sat, 15 Jan 2022 21:46:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:46:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:46:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:46:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:46:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:46:46: 7000000 INFO @ Sat, 15 Jan 2022 21:46:51: 8000000 INFO @ Sat, 15 Jan 2022 21:46:55: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:47:00: 10000000 INFO @ Sat, 15 Jan 2022 21:47:05: 11000000 INFO @ Sat, 15 Jan 2022 21:47:06: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:47:06: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:47:06: #1 total tags in treatment: 11179028 INFO @ Sat, 15 Jan 2022 21:47:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:47:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:47:06: #1 tags after filtering in treatment: 11179028 INFO @ Sat, 15 Jan 2022 21:47:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:47:06: #1 finished! INFO @ Sat, 15 Jan 2022 21:47:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:47:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:47:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:47:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:47:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320606/SRX11320606.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling