Job ID = 14521775 SRX = SRX11320605 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27174809 spots for SRR15008439/SRR15008439.sra Written 27174809 spots for SRR15008439/SRR15008439.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:08 27174809 reads; of these: 27174809 (100.00%) were unpaired; of these: 5034022 (18.52%) aligned 0 times 19766052 (72.74%) aligned exactly 1 time 2374735 (8.74%) aligned >1 times 81.48% overall alignment rate Time searching: 00:03:08 Overall time: 00:03:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12261983 / 22140787 = 0.5538 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:42:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:42:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:42:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:42:23: 1000000 INFO @ Sat, 15 Jan 2022 21:42:28: 2000000 INFO @ Sat, 15 Jan 2022 21:42:34: 3000000 INFO @ Sat, 15 Jan 2022 21:42:39: 4000000 INFO @ Sat, 15 Jan 2022 21:42:44: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:42:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:42:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:42:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:42:50: 6000000 INFO @ Sat, 15 Jan 2022 21:42:53: 1000000 INFO @ Sat, 15 Jan 2022 21:42:57: 7000000 INFO @ Sat, 15 Jan 2022 21:43:00: 2000000 INFO @ Sat, 15 Jan 2022 21:43:03: 8000000 INFO @ Sat, 15 Jan 2022 21:43:06: 3000000 INFO @ Sat, 15 Jan 2022 21:43:09: 9000000 INFO @ Sat, 15 Jan 2022 21:43:12: 4000000 INFO @ Sat, 15 Jan 2022 21:43:14: #1 tag size is determined as 48 bps INFO @ Sat, 15 Jan 2022 21:43:14: #1 tag size = 48 INFO @ Sat, 15 Jan 2022 21:43:14: #1 total tags in treatment: 9878804 INFO @ Sat, 15 Jan 2022 21:43:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:43:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:43:14: #1 tags after filtering in treatment: 9878804 INFO @ Sat, 15 Jan 2022 21:43:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:43:14: #1 finished! INFO @ Sat, 15 Jan 2022 21:43:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:43:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:43:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:43:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:43:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis BedGraph に変換中... needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:43:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:43:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:43:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:43:18: 5000000 INFO @ Sat, 15 Jan 2022 21:43:24: 1000000 INFO @ Sat, 15 Jan 2022 21:43:24: 6000000 INFO @ Sat, 15 Jan 2022 21:43:30: 2000000 INFO @ Sat, 15 Jan 2022 21:43:30: 7000000 INFO @ Sat, 15 Jan 2022 21:43:36: 3000000 INFO @ Sat, 15 Jan 2022 21:43:37: 8000000 INFO @ Sat, 15 Jan 2022 21:43:43: 4000000 INFO @ Sat, 15 Jan 2022 21:43:43: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:43:49: #1 tag size is determined as 48 bps INFO @ Sat, 15 Jan 2022 21:43:49: #1 tag size = 48 INFO @ Sat, 15 Jan 2022 21:43:49: #1 total tags in treatment: 9878804 INFO @ Sat, 15 Jan 2022 21:43:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:43:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:43:49: #1 tags after filtering in treatment: 9878804 INFO @ Sat, 15 Jan 2022 21:43:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:43:49: #1 finished! INFO @ Sat, 15 Jan 2022 21:43:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:43:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:43:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:43:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:43:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:43:50: 5000000 INFO @ Sat, 15 Jan 2022 21:43:57: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:44:02: 7000000 INFO @ Sat, 15 Jan 2022 21:44:08: 8000000 INFO @ Sat, 15 Jan 2022 21:44:13: 9000000 INFO @ Sat, 15 Jan 2022 21:44:18: #1 tag size is determined as 48 bps INFO @ Sat, 15 Jan 2022 21:44:18: #1 tag size = 48 INFO @ Sat, 15 Jan 2022 21:44:18: #1 total tags in treatment: 9878804 INFO @ Sat, 15 Jan 2022 21:44:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:44:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:44:18: #1 tags after filtering in treatment: 9878804 INFO @ Sat, 15 Jan 2022 21:44:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:44:18: #1 finished! INFO @ Sat, 15 Jan 2022 21:44:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:44:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:44:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:44:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:44:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320605/SRX11320605.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling