Job ID = 14521774 SRX = SRX11320604 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 30996429 spots for SRR15008437/SRR15008437.sra Written 30996429 spots for SRR15008437/SRR15008437.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:41 30996429 reads; of these: 30996429 (100.00%) were unpaired; of these: 6841317 (22.07%) aligned 0 times 21476198 (69.29%) aligned exactly 1 time 2678914 (8.64%) aligned >1 times 77.93% overall alignment rate Time searching: 00:03:41 Overall time: 00:03:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13861138 / 24155112 = 0.5738 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:44:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:44:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:44:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:44:05: 1000000 INFO @ Sat, 15 Jan 2022 21:44:10: 2000000 INFO @ Sat, 15 Jan 2022 21:44:14: 3000000 INFO @ Sat, 15 Jan 2022 21:44:19: 4000000 INFO @ Sat, 15 Jan 2022 21:44:24: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:44:28: 6000000 INFO @ Sat, 15 Jan 2022 21:44:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:44:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:44:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:44:33: 7000000 INFO @ Sat, 15 Jan 2022 21:44:36: 1000000 INFO @ Sat, 15 Jan 2022 21:44:38: 8000000 INFO @ Sat, 15 Jan 2022 21:44:41: 2000000 INFO @ Sat, 15 Jan 2022 21:44:43: 9000000 INFO @ Sat, 15 Jan 2022 21:44:46: 3000000 INFO @ Sat, 15 Jan 2022 21:44:48: 10000000 INFO @ Sat, 15 Jan 2022 21:44:49: #1 tag size is determined as 49 bps INFO @ Sat, 15 Jan 2022 21:44:49: #1 tag size = 49 INFO @ Sat, 15 Jan 2022 21:44:49: #1 total tags in treatment: 10293974 INFO @ Sat, 15 Jan 2022 21:44:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:44:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:44:49: #1 tags after filtering in treatment: 10293974 INFO @ Sat, 15 Jan 2022 21:44:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:44:49: #1 finished! INFO @ Sat, 15 Jan 2022 21:44:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:44:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:44:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:44:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:44:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:44:52: 4000000 INFO @ Sat, 15 Jan 2022 21:44:57: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:45:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:45:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:45:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:45:02: 6000000 INFO @ Sat, 15 Jan 2022 21:45:05: 1000000 INFO @ Sat, 15 Jan 2022 21:45:08: 7000000 INFO @ Sat, 15 Jan 2022 21:45:10: 2000000 INFO @ Sat, 15 Jan 2022 21:45:13: 8000000 INFO @ Sat, 15 Jan 2022 21:45:14: 3000000 INFO @ Sat, 15 Jan 2022 21:45:18: 9000000 INFO @ Sat, 15 Jan 2022 21:45:19: 4000000 INFO @ Sat, 15 Jan 2022 21:45:24: 10000000 INFO @ Sat, 15 Jan 2022 21:45:24: 5000000 INFO @ Sat, 15 Jan 2022 21:45:25: #1 tag size is determined as 49 bps INFO @ Sat, 15 Jan 2022 21:45:25: #1 tag size = 49 INFO @ Sat, 15 Jan 2022 21:45:25: #1 total tags in treatment: 10293974 INFO @ Sat, 15 Jan 2022 21:45:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:45:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:45:25: #1 tags after filtering in treatment: 10293974 INFO @ Sat, 15 Jan 2022 21:45:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:45:25: #1 finished! INFO @ Sat, 15 Jan 2022 21:45:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:45:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:45:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:45:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:45:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:45:29: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:45:33: 7000000 INFO @ Sat, 15 Jan 2022 21:45:38: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:45:43: 9000000 INFO @ Sat, 15 Jan 2022 21:45:47: 10000000 INFO @ Sat, 15 Jan 2022 21:45:49: #1 tag size is determined as 49 bps INFO @ Sat, 15 Jan 2022 21:45:49: #1 tag size = 49 INFO @ Sat, 15 Jan 2022 21:45:49: #1 total tags in treatment: 10293974 INFO @ Sat, 15 Jan 2022 21:45:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:45:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:45:49: #1 tags after filtering in treatment: 10293974 INFO @ Sat, 15 Jan 2022 21:45:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:45:49: #1 finished! INFO @ Sat, 15 Jan 2022 21:45:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:45:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:45:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:45:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:45:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320604/SRX11320604.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling