Job ID = 14521773 SRX = SRX11320603 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 37863398 spots for SRR15008435/SRR15008435.sra Written 37863398 spots for SRR15008435/SRR15008435.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:26 37863398 reads; of these: 37863398 (100.00%) were unpaired; of these: 8401869 (22.19%) aligned 0 times 26995979 (71.30%) aligned exactly 1 time 2465550 (6.51%) aligned >1 times 77.81% overall alignment rate Time searching: 00:04:26 Overall time: 00:04:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 17147623 / 29461529 = 0.5820 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:45:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:45:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:45:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:45:57: 1000000 INFO @ Sat, 15 Jan 2022 21:46:03: 2000000 INFO @ Sat, 15 Jan 2022 21:46:09: 3000000 INFO @ Sat, 15 Jan 2022 21:46:15: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:46:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:46:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:46:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:46:21: 5000000 INFO @ Sat, 15 Jan 2022 21:46:27: 1000000 INFO @ Sat, 15 Jan 2022 21:46:28: 6000000 INFO @ Sat, 15 Jan 2022 21:46:34: 2000000 INFO @ Sat, 15 Jan 2022 21:46:34: 7000000 INFO @ Sat, 15 Jan 2022 21:46:40: 3000000 INFO @ Sat, 15 Jan 2022 21:46:41: 8000000 INFO @ Sat, 15 Jan 2022 21:46:47: 4000000 INFO @ Sat, 15 Jan 2022 21:46:47: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:46:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:46:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:46:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:46:54: 5000000 INFO @ Sat, 15 Jan 2022 21:46:54: 10000000 INFO @ Sat, 15 Jan 2022 21:46:59: 1000000 INFO @ Sat, 15 Jan 2022 21:47:01: 6000000 INFO @ Sat, 15 Jan 2022 21:47:02: 11000000 INFO @ Sat, 15 Jan 2022 21:47:07: 2000000 INFO @ Sat, 15 Jan 2022 21:47:09: 7000000 INFO @ Sat, 15 Jan 2022 21:47:09: 12000000 INFO @ Sat, 15 Jan 2022 21:47:11: #1 tag size is determined as 48 bps INFO @ Sat, 15 Jan 2022 21:47:11: #1 tag size = 48 INFO @ Sat, 15 Jan 2022 21:47:11: #1 total tags in treatment: 12313906 INFO @ Sat, 15 Jan 2022 21:47:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:47:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:47:11: #1 tags after filtering in treatment: 12313906 INFO @ Sat, 15 Jan 2022 21:47:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:47:11: #1 finished! INFO @ Sat, 15 Jan 2022 21:47:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:47:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:47:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:47:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:47:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:47:15: 3000000 INFO @ Sat, 15 Jan 2022 21:47:16: 8000000 INFO @ Sat, 15 Jan 2022 21:47:23: 4000000 INFO @ Sat, 15 Jan 2022 21:47:24: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:47:31: 5000000 INFO @ Sat, 15 Jan 2022 21:47:31: 10000000 INFO @ Sat, 15 Jan 2022 21:47:38: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:47:39: 6000000 INFO @ Sat, 15 Jan 2022 21:47:46: 12000000 INFO @ Sat, 15 Jan 2022 21:47:47: 7000000 INFO @ Sat, 15 Jan 2022 21:47:48: #1 tag size is determined as 48 bps INFO @ Sat, 15 Jan 2022 21:47:48: #1 tag size = 48 INFO @ Sat, 15 Jan 2022 21:47:48: #1 total tags in treatment: 12313906 INFO @ Sat, 15 Jan 2022 21:47:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:47:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:47:48: #1 tags after filtering in treatment: 12313906 INFO @ Sat, 15 Jan 2022 21:47:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:47:48: #1 finished! INFO @ Sat, 15 Jan 2022 21:47:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:47:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:47:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:47:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:47:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:47:54: 8000000 INFO @ Sat, 15 Jan 2022 21:48:02: 9000000 INFO @ Sat, 15 Jan 2022 21:48:09: 10000000 INFO @ Sat, 15 Jan 2022 21:48:16: 11000000 INFO @ Sat, 15 Jan 2022 21:48:24: 12000000 INFO @ Sat, 15 Jan 2022 21:48:26: #1 tag size is determined as 48 bps INFO @ Sat, 15 Jan 2022 21:48:26: #1 tag size = 48 INFO @ Sat, 15 Jan 2022 21:48:26: #1 total tags in treatment: 12313906 INFO @ Sat, 15 Jan 2022 21:48:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:48:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:48:26: #1 tags after filtering in treatment: 12313906 INFO @ Sat, 15 Jan 2022 21:48:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:48:26: #1 finished! INFO @ Sat, 15 Jan 2022 21:48:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:48:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:48:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:48:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:48:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320603/SRX11320603.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling