Job ID = 14521770 SRX = SRX11320600 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 24053894 spots for SRR15008430/SRR15008430.sra Written 24053894 spots for SRR15008430/SRR15008430.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:48 24053894 reads; of these: 24053894 (100.00%) were unpaired; of these: 6399232 (26.60%) aligned 0 times 16180277 (67.27%) aligned exactly 1 time 1474385 (6.13%) aligned >1 times 73.40% overall alignment rate Time searching: 00:02:48 Overall time: 00:02:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7938992 / 17654662 = 0.4497 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:44:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:44:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:44:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:44:33: 1000000 INFO @ Sat, 15 Jan 2022 21:44:39: 2000000 INFO @ Sat, 15 Jan 2022 21:44:45: 3000000 INFO @ Sat, 15 Jan 2022 21:44:51: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:44:56: 5000000 INFO @ Sat, 15 Jan 2022 21:44:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:44:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:44:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:45:02: 6000000 INFO @ Sat, 15 Jan 2022 21:45:03: 1000000 INFO @ Sat, 15 Jan 2022 21:45:08: 7000000 INFO @ Sat, 15 Jan 2022 21:45:09: 2000000 INFO @ Sat, 15 Jan 2022 21:45:14: 8000000 INFO @ Sat, 15 Jan 2022 21:45:15: 3000000 INFO @ Sat, 15 Jan 2022 21:45:20: 9000000 INFO @ Sat, 15 Jan 2022 21:45:21: 4000000 INFO @ Sat, 15 Jan 2022 21:45:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:45:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:45:24: #1 total tags in treatment: 9715670 INFO @ Sat, 15 Jan 2022 21:45:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:45:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:45:25: #1 tags after filtering in treatment: 9715670 INFO @ Sat, 15 Jan 2022 21:45:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:45:25: #1 finished! INFO @ Sat, 15 Jan 2022 21:45:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:45:25: #2 looking for paired plus/minus strand peaks... BedGraph に変換中... INFO @ Sat, 15 Jan 2022 21:45:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:45:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:45:25: Process for pairing-model is terminated! WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:45:27: 5000000 INFO @ Sat, 15 Jan 2022 21:45:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:45:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:45:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:45:33: 6000000 INFO @ Sat, 15 Jan 2022 21:45:33: 1000000 INFO @ Sat, 15 Jan 2022 21:45:38: 7000000 INFO @ Sat, 15 Jan 2022 21:45:39: 2000000 INFO @ Sat, 15 Jan 2022 21:45:44: 8000000 INFO @ Sat, 15 Jan 2022 21:45:45: 3000000 INFO @ Sat, 15 Jan 2022 21:45:50: 9000000 INFO @ Sat, 15 Jan 2022 21:45:51: 4000000 INFO @ Sat, 15 Jan 2022 21:45:54: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:45:54: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:45:54: #1 total tags in treatment: 9715670 INFO @ Sat, 15 Jan 2022 21:45:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:45:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:45:54: #1 tags after filtering in treatment: 9715670 INFO @ Sat, 15 Jan 2022 21:45:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:45:54: #1 finished! INFO @ Sat, 15 Jan 2022 21:45:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:45:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:45:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:45:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:45:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:45:57: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:46:03: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:46:08: 7000000 INFO @ Sat, 15 Jan 2022 21:46:14: 8000000 INFO @ Sat, 15 Jan 2022 21:46:19: 9000000 INFO @ Sat, 15 Jan 2022 21:46:23: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:46:23: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:46:23: #1 total tags in treatment: 9715670 INFO @ Sat, 15 Jan 2022 21:46:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:46:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:46:24: #1 tags after filtering in treatment: 9715670 INFO @ Sat, 15 Jan 2022 21:46:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:46:24: #1 finished! INFO @ Sat, 15 Jan 2022 21:46:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:46:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:46:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:46:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:46:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320600/SRX11320600.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling