Job ID = 14521952 SRX = SRX11320599 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 31006288 spots for SRR15008428/SRR15008428.sra Written 31006288 spots for SRR15008428/SRR15008428.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:47 31006288 reads; of these: 31006288 (100.00%) were unpaired; of these: 5204265 (16.78%) aligned 0 times 23700064 (76.44%) aligned exactly 1 time 2101959 (6.78%) aligned >1 times 83.22% overall alignment rate Time searching: 00:03:47 Overall time: 00:03:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14024977 / 25802023 = 0.5436 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:01:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:01:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:01:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:01:56: 1000000 INFO @ Sat, 15 Jan 2022 22:02:02: 2000000 INFO @ Sat, 15 Jan 2022 22:02:07: 3000000 INFO @ Sat, 15 Jan 2022 22:02:12: 4000000 INFO @ Sat, 15 Jan 2022 22:02:18: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:02:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:02:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:02:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:02:24: 6000000 INFO @ Sat, 15 Jan 2022 22:02:27: 1000000 INFO @ Sat, 15 Jan 2022 22:02:30: 7000000 INFO @ Sat, 15 Jan 2022 22:02:34: 2000000 INFO @ Sat, 15 Jan 2022 22:02:36: 8000000 INFO @ Sat, 15 Jan 2022 22:02:40: 3000000 INFO @ Sat, 15 Jan 2022 22:02:42: 9000000 INFO @ Sat, 15 Jan 2022 22:02:46: 4000000 INFO @ Sat, 15 Jan 2022 22:02:48: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:02:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:02:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:02:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:02:52: 5000000 INFO @ Sat, 15 Jan 2022 22:02:55: 11000000 INFO @ Sat, 15 Jan 2022 22:02:57: 1000000 INFO @ Sat, 15 Jan 2022 22:02:58: 6000000 INFO @ Sat, 15 Jan 2022 22:02:59: #1 tag size is determined as 45 bps INFO @ Sat, 15 Jan 2022 22:02:59: #1 tag size = 45 INFO @ Sat, 15 Jan 2022 22:02:59: #1 total tags in treatment: 11777046 INFO @ Sat, 15 Jan 2022 22:02:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:02:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:03:00: #1 tags after filtering in treatment: 11777046 INFO @ Sat, 15 Jan 2022 22:03:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:03:00: #1 finished! INFO @ Sat, 15 Jan 2022 22:03:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:03:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:03:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:03:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:03:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:03:04: 2000000 INFO @ Sat, 15 Jan 2022 22:03:04: 7000000 INFO @ Sat, 15 Jan 2022 22:03:10: 3000000 INFO @ Sat, 15 Jan 2022 22:03:11: 8000000 INFO @ Sat, 15 Jan 2022 22:03:16: 4000000 INFO @ Sat, 15 Jan 2022 22:03:17: 9000000 INFO @ Sat, 15 Jan 2022 22:03:22: 5000000 INFO @ Sat, 15 Jan 2022 22:03:23: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:03:28: 6000000 INFO @ Sat, 15 Jan 2022 22:03:29: 11000000 INFO @ Sat, 15 Jan 2022 22:03:34: #1 tag size is determined as 45 bps INFO @ Sat, 15 Jan 2022 22:03:34: #1 tag size = 45 INFO @ Sat, 15 Jan 2022 22:03:34: #1 total tags in treatment: 11777046 INFO @ Sat, 15 Jan 2022 22:03:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:03:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:03:34: 7000000 INFO @ Sat, 15 Jan 2022 22:03:34: #1 tags after filtering in treatment: 11777046 INFO @ Sat, 15 Jan 2022 22:03:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:03:34: #1 finished! INFO @ Sat, 15 Jan 2022 22:03:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:03:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:03:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:03:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:03:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:03:40: 8000000 INFO @ Sat, 15 Jan 2022 22:03:46: 9000000 INFO @ Sat, 15 Jan 2022 22:03:52: 10000000 INFO @ Sat, 15 Jan 2022 22:03:58: 11000000 INFO @ Sat, 15 Jan 2022 22:04:02: #1 tag size is determined as 45 bps INFO @ Sat, 15 Jan 2022 22:04:02: #1 tag size = 45 INFO @ Sat, 15 Jan 2022 22:04:02: #1 total tags in treatment: 11777046 INFO @ Sat, 15 Jan 2022 22:04:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:04:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:04:02: #1 tags after filtering in treatment: 11777046 INFO @ Sat, 15 Jan 2022 22:04:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:04:02: #1 finished! INFO @ Sat, 15 Jan 2022 22:04:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:04:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:04:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:04:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:04:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320599/SRX11320599.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling