Job ID = 14521951 SRX = SRX11320598 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 32854156 spots for SRR15008427/SRR15008427.sra Written 32854156 spots for SRR15008427/SRR15008427.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:15 32854156 reads; of these: 32854156 (100.00%) were unpaired; of these: 5155808 (15.69%) aligned 0 times 24879227 (75.73%) aligned exactly 1 time 2819121 (8.58%) aligned >1 times 84.31% overall alignment rate Time searching: 00:06:15 Overall time: 00:06:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 16776728 / 27698348 = 0.6057 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:07:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:07:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:07:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:07:18: 1000000 INFO @ Sat, 15 Jan 2022 22:07:30: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:07:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:07:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:07:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:07:41: 3000000 INFO @ Sat, 15 Jan 2022 22:07:49: 1000000 INFO @ Sat, 15 Jan 2022 22:07:53: 4000000 INFO @ Sat, 15 Jan 2022 22:08:02: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:08:04: 5000000 INFO @ Sat, 15 Jan 2022 22:08:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:08:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:08:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:08:14: 1000000 INFO @ Sat, 15 Jan 2022 22:08:16: 3000000 INFO @ Sat, 15 Jan 2022 22:08:16: 6000000 INFO @ Sat, 15 Jan 2022 22:08:23: 2000000 INFO @ Sat, 15 Jan 2022 22:08:28: 7000000 INFO @ Sat, 15 Jan 2022 22:08:30: 4000000 INFO @ Sat, 15 Jan 2022 22:08:32: 3000000 INFO @ Sat, 15 Jan 2022 22:08:40: 8000000 INFO @ Sat, 15 Jan 2022 22:08:41: 4000000 INFO @ Sat, 15 Jan 2022 22:08:45: 5000000 INFO @ Sat, 15 Jan 2022 22:08:50: 5000000 INFO @ Sat, 15 Jan 2022 22:08:52: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:08:59: 6000000 INFO @ Sat, 15 Jan 2022 22:08:59: 6000000 INFO @ Sat, 15 Jan 2022 22:09:05: 10000000 INFO @ Sat, 15 Jan 2022 22:09:08: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:09:13: 7000000 INFO @ Sat, 15 Jan 2022 22:09:15: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:09:15: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:09:15: #1 total tags in treatment: 10921620 INFO @ Sat, 15 Jan 2022 22:09:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:09:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:09:16: #1 tags after filtering in treatment: 10921620 INFO @ Sat, 15 Jan 2022 22:09:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:09:16: #1 finished! INFO @ Sat, 15 Jan 2022 22:09:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:09:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:09:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:09:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:09:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:09:17: 8000000 INFO @ Sat, 15 Jan 2022 22:09:25: 9000000 INFO @ Sat, 15 Jan 2022 22:09:26: 8000000 INFO @ Sat, 15 Jan 2022 22:09:34: 10000000 INFO @ Sat, 15 Jan 2022 22:09:38: 9000000 INFO @ Sat, 15 Jan 2022 22:09:41: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:09:41: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:09:41: #1 total tags in treatment: 10921620 INFO @ Sat, 15 Jan 2022 22:09:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:09:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:09:42: #1 tags after filtering in treatment: 10921620 INFO @ Sat, 15 Jan 2022 22:09:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:09:42: #1 finished! INFO @ Sat, 15 Jan 2022 22:09:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:09:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:09:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:09:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:09:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:09:50: 10000000 INFO @ Sat, 15 Jan 2022 22:10:00: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:10:00: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:10:00: #1 total tags in treatment: 10921620 INFO @ Sat, 15 Jan 2022 22:10:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:10:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:10:00: #1 tags after filtering in treatment: 10921620 INFO @ Sat, 15 Jan 2022 22:10:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:10:00: #1 finished! INFO @ Sat, 15 Jan 2022 22:10:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:10:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:10:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:10:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:10:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11320598/SRX11320598.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling