Job ID = 14521323 SRX = SRX11118942 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10173006 spots for SRR14785642/SRR14785642.sra Written 10173006 spots for SRR14785642/SRR14785642.sra Read 8323415 spots for SRR14785643/SRR14785643.sra Written 8323415 spots for SRR14785643/SRR14785643.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:28 18496421 reads; of these: 18496421 (100.00%) were paired; of these: 5087533 (27.51%) aligned concordantly 0 times 11850884 (64.07%) aligned concordantly exactly 1 time 1558004 (8.42%) aligned concordantly >1 times ---- 5087533 pairs aligned concordantly 0 times; of these: 1569970 (30.86%) aligned discordantly 1 time ---- 3517563 pairs aligned 0 times concordantly or discordantly; of these: 7035126 mates make up the pairs; of these: 5975025 (84.93%) aligned 0 times 460024 (6.54%) aligned exactly 1 time 600077 (8.53%) aligned >1 times 83.85% overall alignment rate Time searching: 00:15:28 Overall time: 00:15:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2282761 / 14842709 = 0.1538 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:23:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:23:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:23:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:23:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:23:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:23:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:23:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:23:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:23:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:23:19: 1000000 INFO @ Sat, 15 Jan 2022 21:23:19: 1000000 INFO @ Sat, 15 Jan 2022 21:23:19: 1000000 INFO @ Sat, 15 Jan 2022 21:23:28: 2000000 INFO @ Sat, 15 Jan 2022 21:23:28: 2000000 INFO @ Sat, 15 Jan 2022 21:23:28: 2000000 INFO @ Sat, 15 Jan 2022 21:23:35: 3000000 INFO @ Sat, 15 Jan 2022 21:23:37: 3000000 INFO @ Sat, 15 Jan 2022 21:23:37: 3000000 INFO @ Sat, 15 Jan 2022 21:23:43: 4000000 INFO @ Sat, 15 Jan 2022 21:23:46: 4000000 INFO @ Sat, 15 Jan 2022 21:23:46: 4000000 INFO @ Sat, 15 Jan 2022 21:23:51: 5000000 INFO @ Sat, 15 Jan 2022 21:23:55: 5000000 INFO @ Sat, 15 Jan 2022 21:23:55: 5000000 INFO @ Sat, 15 Jan 2022 21:23:58: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:24:02: 6000000 INFO @ Sat, 15 Jan 2022 21:24:03: 6000000 INFO @ Sat, 15 Jan 2022 21:24:05: 7000000 INFO @ Sat, 15 Jan 2022 21:24:10: 7000000 INFO @ Sat, 15 Jan 2022 21:24:11: 7000000 INFO @ Sat, 15 Jan 2022 21:24:12: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:24:17: 8000000 INFO @ Sat, 15 Jan 2022 21:24:18: 8000000 INFO @ Sat, 15 Jan 2022 21:24:19: 9000000 INFO @ Sat, 15 Jan 2022 21:24:24: 9000000 INFO @ Sat, 15 Jan 2022 21:24:25: 9000000 INFO @ Sat, 15 Jan 2022 21:24:26: 10000000 INFO @ Sat, 15 Jan 2022 21:24:31: 10000000 INFO @ Sat, 15 Jan 2022 21:24:33: 10000000 INFO @ Sat, 15 Jan 2022 21:24:33: 11000000 INFO @ Sat, 15 Jan 2022 21:24:38: 11000000 INFO @ Sat, 15 Jan 2022 21:24:40: 11000000 INFO @ Sat, 15 Jan 2022 21:24:41: 12000000 INFO @ Sat, 15 Jan 2022 21:24:45: 12000000 INFO @ Sat, 15 Jan 2022 21:24:47: 12000000 INFO @ Sat, 15 Jan 2022 21:24:48: 13000000 INFO @ Sat, 15 Jan 2022 21:24:52: 13000000 INFO @ Sat, 15 Jan 2022 21:24:55: 13000000 INFO @ Sat, 15 Jan 2022 21:24:56: 14000000 INFO @ Sat, 15 Jan 2022 21:25:00: 14000000 INFO @ Sat, 15 Jan 2022 21:25:03: 14000000 INFO @ Sat, 15 Jan 2022 21:25:04: 15000000 INFO @ Sat, 15 Jan 2022 21:25:08: 15000000 INFO @ Sat, 15 Jan 2022 21:25:10: 15000000 INFO @ Sat, 15 Jan 2022 21:25:11: 16000000 INFO @ Sat, 15 Jan 2022 21:25:15: 16000000 INFO @ Sat, 15 Jan 2022 21:25:18: 16000000 INFO @ Sat, 15 Jan 2022 21:25:19: 17000000 INFO @ Sat, 15 Jan 2022 21:25:23: 17000000 INFO @ Sat, 15 Jan 2022 21:25:26: 17000000 INFO @ Sat, 15 Jan 2022 21:25:27: 18000000 INFO @ Sat, 15 Jan 2022 21:25:30: 18000000 INFO @ Sat, 15 Jan 2022 21:25:33: 18000000 INFO @ Sat, 15 Jan 2022 21:25:35: 19000000 INFO @ Sat, 15 Jan 2022 21:25:38: 19000000 INFO @ Sat, 15 Jan 2022 21:25:41: 19000000 INFO @ Sat, 15 Jan 2022 21:25:42: 20000000 INFO @ Sat, 15 Jan 2022 21:25:45: 20000000 INFO @ Sat, 15 Jan 2022 21:25:48: 20000000 INFO @ Sat, 15 Jan 2022 21:25:49: 21000000 INFO @ Sat, 15 Jan 2022 21:25:52: 21000000 INFO @ Sat, 15 Jan 2022 21:25:55: 21000000 INFO @ Sat, 15 Jan 2022 21:25:56: 22000000 INFO @ Sat, 15 Jan 2022 21:25:59: 22000000 INFO @ Sat, 15 Jan 2022 21:26:02: 22000000 INFO @ Sat, 15 Jan 2022 21:26:03: 23000000 INFO @ Sat, 15 Jan 2022 21:26:06: 23000000 INFO @ Sat, 15 Jan 2022 21:26:10: 23000000 INFO @ Sat, 15 Jan 2022 21:26:10: 24000000 INFO @ Sat, 15 Jan 2022 21:26:13: 24000000 INFO @ Sat, 15 Jan 2022 21:26:17: 24000000 INFO @ Sat, 15 Jan 2022 21:26:18: 25000000 INFO @ Sat, 15 Jan 2022 21:26:20: 25000000 INFO @ Sat, 15 Jan 2022 21:26:24: 25000000 INFO @ Sat, 15 Jan 2022 21:26:25: 26000000 INFO @ Sat, 15 Jan 2022 21:26:28: 26000000 INFO @ Sat, 15 Jan 2022 21:26:28: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:26:28: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:26:28: #1 total tags in treatment: 11332551 INFO @ Sat, 15 Jan 2022 21:26:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:26:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:26:29: #1 tags after filtering in treatment: 8095854 INFO @ Sat, 15 Jan 2022 21:26:29: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:26:29: #1 finished! INFO @ Sat, 15 Jan 2022 21:26:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:26:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:26:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:26:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:26:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:26:31: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:26:31: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:26:31: #1 total tags in treatment: 11332551 INFO @ Sat, 15 Jan 2022 21:26:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:26:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:26:31: #1 tags after filtering in treatment: 8095854 INFO @ Sat, 15 Jan 2022 21:26:31: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:26:31: #1 finished! INFO @ Sat, 15 Jan 2022 21:26:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:26:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:26:32: 26000000 INFO @ Sat, 15 Jan 2022 21:26:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:26:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:26:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:26:35: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:26:35: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:26:35: #1 total tags in treatment: 11332551 INFO @ Sat, 15 Jan 2022 21:26:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:26:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:26:35: #1 tags after filtering in treatment: 8095854 INFO @ Sat, 15 Jan 2022 21:26:35: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:26:35: #1 finished! INFO @ Sat, 15 Jan 2022 21:26:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:26:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:26:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:26:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:26:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118942/SRX11118942.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling