Job ID = 14521298 SRX = SRX11118939 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10368814 spots for SRR14785636/SRR14785636.sra Written 10368814 spots for SRR14785636/SRR14785636.sra Read 8422225 spots for SRR14785637/SRR14785637.sra Written 8422225 spots for SRR14785637/SRR14785637.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:47 18791039 reads; of these: 18791039 (100.00%) were paired; of these: 8199664 (43.64%) aligned concordantly 0 times 9135890 (48.62%) aligned concordantly exactly 1 time 1455485 (7.75%) aligned concordantly >1 times ---- 8199664 pairs aligned concordantly 0 times; of these: 1121705 (13.68%) aligned discordantly 1 time ---- 7077959 pairs aligned 0 times concordantly or discordantly; of these: 14155918 mates make up the pairs; of these: 13037010 (92.10%) aligned 0 times 594689 (4.20%) aligned exactly 1 time 524219 (3.70%) aligned >1 times 65.31% overall alignment rate Time searching: 00:12:47 Overall time: 00:12:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2031522 / 11618134 = 0.1749 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:13:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:13:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:13:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:13:21: 1000000 INFO @ Sat, 15 Jan 2022 21:13:26: 2000000 INFO @ Sat, 15 Jan 2022 21:13:32: 3000000 INFO @ Sat, 15 Jan 2022 21:13:37: 4000000 INFO @ Sat, 15 Jan 2022 21:13:42: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:13:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:13:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:13:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:13:47: 6000000 INFO @ Sat, 15 Jan 2022 21:13:51: 1000000 INFO @ Sat, 15 Jan 2022 21:13:53: 7000000 INFO @ Sat, 15 Jan 2022 21:13:57: 2000000 INFO @ Sat, 15 Jan 2022 21:13:58: 8000000 INFO @ Sat, 15 Jan 2022 21:14:02: 3000000 INFO @ Sat, 15 Jan 2022 21:14:04: 9000000 INFO @ Sat, 15 Jan 2022 21:14:08: 4000000 INFO @ Sat, 15 Jan 2022 21:14:10: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:14:14: 5000000 INFO @ Sat, 15 Jan 2022 21:14:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:14:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:14:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:14:15: 11000000 INFO @ Sat, 15 Jan 2022 21:14:19: 6000000 INFO @ Sat, 15 Jan 2022 21:14:21: 12000000 INFO @ Sat, 15 Jan 2022 21:14:22: 1000000 INFO @ Sat, 15 Jan 2022 21:14:25: 7000000 INFO @ Sat, 15 Jan 2022 21:14:27: 13000000 INFO @ Sat, 15 Jan 2022 21:14:28: 2000000 INFO @ Sat, 15 Jan 2022 21:14:31: 8000000 INFO @ Sat, 15 Jan 2022 21:14:33: 14000000 INFO @ Sat, 15 Jan 2022 21:14:35: 3000000 INFO @ Sat, 15 Jan 2022 21:14:37: 9000000 INFO @ Sat, 15 Jan 2022 21:14:38: 15000000 INFO @ Sat, 15 Jan 2022 21:14:41: 4000000 INFO @ Sat, 15 Jan 2022 21:14:43: 10000000 INFO @ Sat, 15 Jan 2022 21:14:44: 16000000 INFO @ Sat, 15 Jan 2022 21:14:48: 5000000 INFO @ Sat, 15 Jan 2022 21:14:49: 11000000 INFO @ Sat, 15 Jan 2022 21:14:50: 17000000 INFO @ Sat, 15 Jan 2022 21:14:54: 12000000 INFO @ Sat, 15 Jan 2022 21:14:54: 6000000 INFO @ Sat, 15 Jan 2022 21:14:56: 18000000 INFO @ Sat, 15 Jan 2022 21:15:00: 13000000 INFO @ Sat, 15 Jan 2022 21:15:01: 7000000 INFO @ Sat, 15 Jan 2022 21:15:01: 19000000 INFO @ Sat, 15 Jan 2022 21:15:06: 14000000 INFO @ Sat, 15 Jan 2022 21:15:07: 20000000 INFO @ Sat, 15 Jan 2022 21:15:08: 8000000 INFO @ Sat, 15 Jan 2022 21:15:10: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:15:10: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:15:10: #1 total tags in treatment: 8733816 INFO @ Sat, 15 Jan 2022 21:15:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:15:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:15:10: #1 tags after filtering in treatment: 6542418 INFO @ Sat, 15 Jan 2022 21:15:10: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 21:15:10: #1 finished! INFO @ Sat, 15 Jan 2022 21:15:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:15:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:15:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:15:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:15:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:15:12: 15000000 INFO @ Sat, 15 Jan 2022 21:15:14: 9000000 INFO @ Sat, 15 Jan 2022 21:15:18: 16000000 INFO @ Sat, 15 Jan 2022 21:15:21: 10000000 INFO @ Sat, 15 Jan 2022 21:15:23: 17000000 INFO @ Sat, 15 Jan 2022 21:15:27: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:15:29: 18000000 INFO @ Sat, 15 Jan 2022 21:15:34: 12000000 INFO @ Sat, 15 Jan 2022 21:15:35: 19000000 INFO @ Sat, 15 Jan 2022 21:15:40: 13000000 INFO @ Sat, 15 Jan 2022 21:15:41: 20000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:15:43: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:15:43: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:15:43: #1 total tags in treatment: 8733816 INFO @ Sat, 15 Jan 2022 21:15:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:15:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:15:44: #1 tags after filtering in treatment: 6542418 INFO @ Sat, 15 Jan 2022 21:15:44: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 21:15:44: #1 finished! INFO @ Sat, 15 Jan 2022 21:15:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:15:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:15:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:15:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:15:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:15:47: 14000000 INFO @ Sat, 15 Jan 2022 21:15:53: 15000000 INFO @ Sat, 15 Jan 2022 21:16:00: 16000000 INFO @ Sat, 15 Jan 2022 21:16:07: 17000000 INFO @ Sat, 15 Jan 2022 21:16:14: 18000000 INFO @ Sat, 15 Jan 2022 21:16:21: 19000000 INFO @ Sat, 15 Jan 2022 21:16:27: 20000000 INFO @ Sat, 15 Jan 2022 21:16:30: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:16:30: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:16:30: #1 total tags in treatment: 8733816 INFO @ Sat, 15 Jan 2022 21:16:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:16:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:16:30: #1 tags after filtering in treatment: 6542418 INFO @ Sat, 15 Jan 2022 21:16:30: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 21:16:30: #1 finished! INFO @ Sat, 15 Jan 2022 21:16:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:16:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:16:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:16:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:16:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX11118939/SRX11118939.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling