Job ID = 14521457 SRX = SRX10828591 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 5523982 spots for SRR14480416/SRR14480416.sra Written 5523982 spots for SRR14480416/SRR14480416.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:51 5523982 reads; of these: 5523982 (100.00%) were paired; of these: 630322 (11.41%) aligned concordantly 0 times 4206723 (76.15%) aligned concordantly exactly 1 time 686937 (12.44%) aligned concordantly >1 times ---- 630322 pairs aligned concordantly 0 times; of these: 107745 (17.09%) aligned discordantly 1 time ---- 522577 pairs aligned 0 times concordantly or discordantly; of these: 1045154 mates make up the pairs; of these: 899427 (86.06%) aligned 0 times 94066 (9.00%) aligned exactly 1 time 51661 (4.94%) aligned >1 times 91.86% overall alignment rate Time searching: 00:02:51 Overall time: 00:02:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 504996 / 4957012 = 0.1019 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:05:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:05:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:05:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:05:50: 1000000 INFO @ Sat, 15 Jan 2022 21:05:55: 2000000 INFO @ Sat, 15 Jan 2022 21:06:00: 3000000 INFO @ Sat, 15 Jan 2022 21:06:05: 4000000 INFO @ Sat, 15 Jan 2022 21:06:10: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:06:15: 6000000 INFO @ Sat, 15 Jan 2022 21:06:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:06:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:06:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:06:21: 7000000 INFO @ Sat, 15 Jan 2022 21:06:22: 1000000 INFO @ Sat, 15 Jan 2022 21:06:28: 8000000 INFO @ Sat, 15 Jan 2022 21:06:28: 2000000 INFO @ Sat, 15 Jan 2022 21:06:34: 9000000 INFO @ Sat, 15 Jan 2022 21:06:35: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:06:35: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:06:35: #1 total tags in treatment: 4392633 INFO @ Sat, 15 Jan 2022 21:06:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:06:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:06:35: 3000000 INFO @ Sat, 15 Jan 2022 21:06:35: #1 tags after filtering in treatment: 3045750 INFO @ Sat, 15 Jan 2022 21:06:35: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 21:06:35: #1 finished! INFO @ Sat, 15 Jan 2022 21:06:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:06:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:06:35: #2 number of paired peaks: 4 WARNING @ Sat, 15 Jan 2022 21:06:35: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:06:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:06:40: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:06:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:06:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:06:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:06:46: 5000000 INFO @ Sat, 15 Jan 2022 21:06:52: 6000000 INFO @ Sat, 15 Jan 2022 21:06:53: 1000000 INFO @ Sat, 15 Jan 2022 21:06:58: 7000000 INFO @ Sat, 15 Jan 2022 21:07:00: 2000000 INFO @ Sat, 15 Jan 2022 21:07:05: 8000000 INFO @ Sat, 15 Jan 2022 21:07:07: 3000000 INFO @ Sat, 15 Jan 2022 21:07:11: 9000000 INFO @ Sat, 15 Jan 2022 21:07:12: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:07:12: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:07:12: #1 total tags in treatment: 4392633 INFO @ Sat, 15 Jan 2022 21:07:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:07:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:07:12: #1 tags after filtering in treatment: 3045750 INFO @ Sat, 15 Jan 2022 21:07:12: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 21:07:12: #1 finished! INFO @ Sat, 15 Jan 2022 21:07:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:07:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:07:12: #2 number of paired peaks: 4 WARNING @ Sat, 15 Jan 2022 21:07:12: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:07:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:07:14: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:07:21: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:07:27: 6000000 INFO @ Sat, 15 Jan 2022 21:07:34: 7000000 INFO @ Sat, 15 Jan 2022 21:07:41: 8000000 INFO @ Sat, 15 Jan 2022 21:07:47: 9000000 INFO @ Sat, 15 Jan 2022 21:07:48: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:07:48: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:07:48: #1 total tags in treatment: 4392633 INFO @ Sat, 15 Jan 2022 21:07:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:07:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:07:48: #1 tags after filtering in treatment: 3045750 INFO @ Sat, 15 Jan 2022 21:07:48: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 21:07:48: #1 finished! INFO @ Sat, 15 Jan 2022 21:07:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:07:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:07:48: #2 number of paired peaks: 4 WARNING @ Sat, 15 Jan 2022 21:07:48: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:07:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828591/SRX10828591.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling