Job ID = 14521456 SRX = SRX10828590 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10121918 spots for SRR14480415/SRR14480415.sra Written 10121918 spots for SRR14480415/SRR14480415.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:20 10121918 reads; of these: 10121918 (100.00%) were paired; of these: 1570576 (15.52%) aligned concordantly 0 times 7184619 (70.98%) aligned concordantly exactly 1 time 1366723 (13.50%) aligned concordantly >1 times ---- 1570576 pairs aligned concordantly 0 times; of these: 408276 (26.00%) aligned discordantly 1 time ---- 1162300 pairs aligned 0 times concordantly or discordantly; of these: 2324600 mates make up the pairs; of these: 1854605 (79.78%) aligned 0 times 278260 (11.97%) aligned exactly 1 time 191735 (8.25%) aligned >1 times 90.84% overall alignment rate Time searching: 00:05:20 Overall time: 00:05:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1273024 / 8784172 = 0.1449 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:09:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:09:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:09:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:09:39: 1000000 INFO @ Sat, 15 Jan 2022 21:09:44: 2000000 INFO @ Sat, 15 Jan 2022 21:09:49: 3000000 INFO @ Sat, 15 Jan 2022 21:09:54: 4000000 INFO @ Sat, 15 Jan 2022 21:09:59: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:10:03: 6000000 INFO @ Sat, 15 Jan 2022 21:10:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:10:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:10:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:10:09: 7000000 INFO @ Sat, 15 Jan 2022 21:10:09: 1000000 INFO @ Sat, 15 Jan 2022 21:10:14: 8000000 INFO @ Sat, 15 Jan 2022 21:10:15: 2000000 INFO @ Sat, 15 Jan 2022 21:10:20: 9000000 INFO @ Sat, 15 Jan 2022 21:10:20: 3000000 INFO @ Sat, 15 Jan 2022 21:10:25: 10000000 INFO @ Sat, 15 Jan 2022 21:10:26: 4000000 INFO @ Sat, 15 Jan 2022 21:10:31: 11000000 INFO @ Sat, 15 Jan 2022 21:10:31: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:10:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:10:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:10:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:10:36: 12000000 INFO @ Sat, 15 Jan 2022 21:10:37: 6000000 INFO @ Sat, 15 Jan 2022 21:10:41: 1000000 INFO @ Sat, 15 Jan 2022 21:10:43: 13000000 INFO @ Sat, 15 Jan 2022 21:10:43: 7000000 INFO @ Sat, 15 Jan 2022 21:10:48: 2000000 INFO @ Sat, 15 Jan 2022 21:10:49: 14000000 INFO @ Sat, 15 Jan 2022 21:10:50: 8000000 INFO @ Sat, 15 Jan 2022 21:10:55: 3000000 INFO @ Sat, 15 Jan 2022 21:10:55: 15000000 INFO @ Sat, 15 Jan 2022 21:10:56: 9000000 INFO @ Sat, 15 Jan 2022 21:11:00: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:11:00: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:11:00: #1 total tags in treatment: 7301795 INFO @ Sat, 15 Jan 2022 21:11:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:11:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:11:00: #1 tags after filtering in treatment: 4349339 INFO @ Sat, 15 Jan 2022 21:11:00: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 21:11:00: #1 finished! INFO @ Sat, 15 Jan 2022 21:11:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:11:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:11:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:11:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:11:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:11:02: 4000000 INFO @ Sat, 15 Jan 2022 21:11:02: 10000000 INFO @ Sat, 15 Jan 2022 21:11:08: 11000000 INFO @ Sat, 15 Jan 2022 21:11:09: 5000000 INFO @ Sat, 15 Jan 2022 21:11:14: 12000000 INFO @ Sat, 15 Jan 2022 21:11:15: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:11:20: 13000000 INFO @ Sat, 15 Jan 2022 21:11:22: 7000000 INFO @ Sat, 15 Jan 2022 21:11:26: 14000000 INFO @ Sat, 15 Jan 2022 21:11:29: 8000000 INFO @ Sat, 15 Jan 2022 21:11:32: 15000000 INFO @ Sat, 15 Jan 2022 21:11:36: 9000000 INFO @ Sat, 15 Jan 2022 21:11:37: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:11:37: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:11:37: #1 total tags in treatment: 7301795 INFO @ Sat, 15 Jan 2022 21:11:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:11:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:11:38: #1 tags after filtering in treatment: 4349339 INFO @ Sat, 15 Jan 2022 21:11:38: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 21:11:38: #1 finished! INFO @ Sat, 15 Jan 2022 21:11:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:11:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:11:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:11:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:11:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:11:43: 10000000 INFO @ Sat, 15 Jan 2022 21:11:49: 11000000 INFO @ Sat, 15 Jan 2022 21:11:55: 12000000 INFO @ Sat, 15 Jan 2022 21:12:01: 13000000 INFO @ Sat, 15 Jan 2022 21:12:07: 14000000 INFO @ Sat, 15 Jan 2022 21:12:14: 15000000 INFO @ Sat, 15 Jan 2022 21:12:19: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:12:19: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:12:19: #1 total tags in treatment: 7301795 INFO @ Sat, 15 Jan 2022 21:12:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:12:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:12:19: #1 tags after filtering in treatment: 4349339 INFO @ Sat, 15 Jan 2022 21:12:19: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 21:12:19: #1 finished! INFO @ Sat, 15 Jan 2022 21:12:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:12:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:12:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:12:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:12:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828590/SRX10828590.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling