Job ID = 14521434 SRX = SRX10828587 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8463340 spots for SRR14480412/SRR14480412.sra Written 8463340 spots for SRR14480412/SRR14480412.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:54 8463340 reads; of these: 8463340 (100.00%) were paired; of these: 1357478 (16.04%) aligned concordantly 0 times 5965803 (70.49%) aligned concordantly exactly 1 time 1140059 (13.47%) aligned concordantly >1 times ---- 1357478 pairs aligned concordantly 0 times; of these: 308129 (22.70%) aligned discordantly 1 time ---- 1049349 pairs aligned 0 times concordantly or discordantly; of these: 2098698 mates make up the pairs; of these: 1671149 (79.63%) aligned 0 times 263862 (12.57%) aligned exactly 1 time 163687 (7.80%) aligned >1 times 90.13% overall alignment rate Time searching: 00:06:54 Overall time: 00:06:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1019648 / 7282765 = 0.1400 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:10:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:10:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:10:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:11:01: 1000000 INFO @ Sat, 15 Jan 2022 21:11:08: 2000000 INFO @ Sat, 15 Jan 2022 21:11:14: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:11:21: 4000000 INFO @ Sat, 15 Jan 2022 21:11:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:11:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:11:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:11:28: 5000000 INFO @ Sat, 15 Jan 2022 21:11:31: 1000000 INFO @ Sat, 15 Jan 2022 21:11:35: 6000000 INFO @ Sat, 15 Jan 2022 21:11:38: 2000000 INFO @ Sat, 15 Jan 2022 21:11:41: 7000000 INFO @ Sat, 15 Jan 2022 21:11:46: 3000000 INFO @ Sat, 15 Jan 2022 21:11:48: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:11:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:11:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:11:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:11:53: 4000000 INFO @ Sat, 15 Jan 2022 21:11:55: 9000000 INFO @ Sat, 15 Jan 2022 21:12:01: 5000000 INFO @ Sat, 15 Jan 2022 21:12:01: 1000000 INFO @ Sat, 15 Jan 2022 21:12:02: 10000000 INFO @ Sat, 15 Jan 2022 21:12:09: 6000000 INFO @ Sat, 15 Jan 2022 21:12:09: 2000000 INFO @ Sat, 15 Jan 2022 21:12:10: 11000000 INFO @ Sat, 15 Jan 2022 21:12:16: 7000000 INFO @ Sat, 15 Jan 2022 21:12:17: 3000000 INFO @ Sat, 15 Jan 2022 21:12:17: 12000000 INFO @ Sat, 15 Jan 2022 21:12:24: 8000000 INFO @ Sat, 15 Jan 2022 21:12:25: 4000000 INFO @ Sat, 15 Jan 2022 21:12:26: 13000000 INFO @ Sat, 15 Jan 2022 21:12:27: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:12:27: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:12:27: #1 total tags in treatment: 6100763 INFO @ Sat, 15 Jan 2022 21:12:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:12:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:12:28: #1 tags after filtering in treatment: 3832446 INFO @ Sat, 15 Jan 2022 21:12:28: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 21:12:28: #1 finished! INFO @ Sat, 15 Jan 2022 21:12:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:12:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:12:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:12:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:12:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:12:31: 9000000 INFO @ Sat, 15 Jan 2022 21:12:32: 5000000 INFO @ Sat, 15 Jan 2022 21:12:39: 10000000 INFO @ Sat, 15 Jan 2022 21:12:39: 6000000 INFO @ Sat, 15 Jan 2022 21:12:46: 11000000 INFO @ Sat, 15 Jan 2022 21:12:46: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:12:53: 8000000 INFO @ Sat, 15 Jan 2022 21:12:53: 12000000 INFO @ Sat, 15 Jan 2022 21:12:59: 9000000 INFO @ Sat, 15 Jan 2022 21:13:00: 13000000 INFO @ Sat, 15 Jan 2022 21:13:02: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:13:02: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:13:02: #1 total tags in treatment: 6100763 INFO @ Sat, 15 Jan 2022 21:13:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:13:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:13:02: #1 tags after filtering in treatment: 3832446 INFO @ Sat, 15 Jan 2022 21:13:02: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 21:13:02: #1 finished! INFO @ Sat, 15 Jan 2022 21:13:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:13:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:13:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:13:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:13:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:13:05: 10000000 INFO @ Sat, 15 Jan 2022 21:13:12: 11000000 INFO @ Sat, 15 Jan 2022 21:13:19: 12000000 INFO @ Sat, 15 Jan 2022 21:13:25: 13000000 INFO @ Sat, 15 Jan 2022 21:13:27: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:13:27: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:13:27: #1 total tags in treatment: 6100763 INFO @ Sat, 15 Jan 2022 21:13:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:13:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:13:27: #1 tags after filtering in treatment: 3832446 INFO @ Sat, 15 Jan 2022 21:13:27: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 21:13:27: #1 finished! INFO @ Sat, 15 Jan 2022 21:13:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:13:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:13:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:13:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:13:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828587/SRX10828587.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling