Job ID = 14521433 SRX = SRX10828586 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8316031 spots for SRR14480411/SRR14480411.sra Written 8316031 spots for SRR14480411/SRR14480411.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:29 8316031 reads; of these: 8316031 (100.00%) were paired; of these: 1191769 (14.33%) aligned concordantly 0 times 6001797 (72.17%) aligned concordantly exactly 1 time 1122465 (13.50%) aligned concordantly >1 times ---- 1191769 pairs aligned concordantly 0 times; of these: 308862 (25.92%) aligned discordantly 1 time ---- 882907 pairs aligned 0 times concordantly or discordantly; of these: 1765814 mates make up the pairs; of these: 1365611 (77.34%) aligned 0 times 246009 (13.93%) aligned exactly 1 time 154194 (8.73%) aligned >1 times 91.79% overall alignment rate Time searching: 00:06:29 Overall time: 00:06:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 978333 / 7275439 = 0.1345 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:09:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:09:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:09:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:09:56: 1000000 INFO @ Sat, 15 Jan 2022 21:10:03: 2000000 INFO @ Sat, 15 Jan 2022 21:10:11: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:10:18: 4000000 INFO @ Sat, 15 Jan 2022 21:10:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:10:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:10:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:10:24: 5000000 INFO @ Sat, 15 Jan 2022 21:10:26: 1000000 INFO @ Sat, 15 Jan 2022 21:10:32: 6000000 INFO @ Sat, 15 Jan 2022 21:10:34: 2000000 INFO @ Sat, 15 Jan 2022 21:10:40: 7000000 INFO @ Sat, 15 Jan 2022 21:10:42: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:10:48: 8000000 INFO @ Sat, 15 Jan 2022 21:10:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:10:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:10:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:10:50: 4000000 INFO @ Sat, 15 Jan 2022 21:10:56: 9000000 INFO @ Sat, 15 Jan 2022 21:10:57: 1000000 INFO @ Sat, 15 Jan 2022 21:10:58: 5000000 INFO @ Sat, 15 Jan 2022 21:11:04: 10000000 INFO @ Sat, 15 Jan 2022 21:11:06: 2000000 INFO @ Sat, 15 Jan 2022 21:11:07: 6000000 INFO @ Sat, 15 Jan 2022 21:11:13: 11000000 INFO @ Sat, 15 Jan 2022 21:11:15: 3000000 INFO @ Sat, 15 Jan 2022 21:11:15: 7000000 INFO @ Sat, 15 Jan 2022 21:11:22: 12000000 INFO @ Sat, 15 Jan 2022 21:11:23: 4000000 INFO @ Sat, 15 Jan 2022 21:11:24: 8000000 INFO @ Sat, 15 Jan 2022 21:11:30: 13000000 INFO @ Sat, 15 Jan 2022 21:11:31: 5000000 INFO @ Sat, 15 Jan 2022 21:11:32: 9000000 INFO @ Sat, 15 Jan 2022 21:11:32: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:11:32: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:11:32: #1 total tags in treatment: 6159377 INFO @ Sat, 15 Jan 2022 21:11:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:11:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:11:32: #1 tags after filtering in treatment: 3870270 INFO @ Sat, 15 Jan 2022 21:11:32: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 21:11:32: #1 finished! INFO @ Sat, 15 Jan 2022 21:11:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:11:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:11:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:11:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:11:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:11:39: 6000000 INFO @ Sat, 15 Jan 2022 21:11:40: 10000000 INFO @ Sat, 15 Jan 2022 21:11:47: 7000000 INFO @ Sat, 15 Jan 2022 21:11:47: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:11:55: 8000000 INFO @ Sat, 15 Jan 2022 21:11:55: 12000000 INFO @ Sat, 15 Jan 2022 21:12:03: 9000000 INFO @ Sat, 15 Jan 2022 21:12:03: 13000000 INFO @ Sat, 15 Jan 2022 21:12:06: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:12:06: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:12:06: #1 total tags in treatment: 6159377 INFO @ Sat, 15 Jan 2022 21:12:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:12:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:12:06: #1 tags after filtering in treatment: 3870270 INFO @ Sat, 15 Jan 2022 21:12:06: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 21:12:06: #1 finished! INFO @ Sat, 15 Jan 2022 21:12:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:12:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:12:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:12:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:12:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:12:11: 10000000 INFO @ Sat, 15 Jan 2022 21:12:18: 11000000 INFO @ Sat, 15 Jan 2022 21:12:26: 12000000 INFO @ Sat, 15 Jan 2022 21:12:33: 13000000 INFO @ Sat, 15 Jan 2022 21:12:35: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:12:35: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:12:35: #1 total tags in treatment: 6159377 INFO @ Sat, 15 Jan 2022 21:12:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:12:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:12:35: #1 tags after filtering in treatment: 3870270 INFO @ Sat, 15 Jan 2022 21:12:35: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 21:12:35: #1 finished! INFO @ Sat, 15 Jan 2022 21:12:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:12:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:12:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:12:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:12:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828586/SRX10828586.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling