Job ID = 14521432 SRX = SRX10828585 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8035363 spots for SRR14480410/SRR14480410.sra Written 8035363 spots for SRR14480410/SRR14480410.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:21 8035363 reads; of these: 8035363 (100.00%) were paired; of these: 1296318 (16.13%) aligned concordantly 0 times 5619521 (69.93%) aligned concordantly exactly 1 time 1119524 (13.93%) aligned concordantly >1 times ---- 1296318 pairs aligned concordantly 0 times; of these: 343372 (26.49%) aligned discordantly 1 time ---- 952946 pairs aligned 0 times concordantly or discordantly; of these: 1905892 mates make up the pairs; of these: 1547908 (81.22%) aligned 0 times 196081 (10.29%) aligned exactly 1 time 161903 (8.49%) aligned >1 times 90.37% overall alignment rate Time searching: 00:04:21 Overall time: 00:04:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 957150 / 6963724 = 0.1374 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:06:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:06:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:06:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:06:35: 1000000 INFO @ Sat, 15 Jan 2022 21:06:41: 2000000 INFO @ Sat, 15 Jan 2022 21:06:46: 3000000 INFO @ Sat, 15 Jan 2022 21:06:52: 4000000 INFO @ Sat, 15 Jan 2022 21:06:57: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:06:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:06:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:06:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:07:03: 6000000 INFO @ Sat, 15 Jan 2022 21:07:07: 1000000 INFO @ Sat, 15 Jan 2022 21:07:09: 7000000 INFO @ Sat, 15 Jan 2022 21:07:14: 2000000 INFO @ Sat, 15 Jan 2022 21:07:15: 8000000 INFO @ Sat, 15 Jan 2022 21:07:21: 3000000 INFO @ Sat, 15 Jan 2022 21:07:22: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:07:28: 10000000 INFO @ Sat, 15 Jan 2022 21:07:29: 4000000 INFO @ Sat, 15 Jan 2022 21:07:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:07:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:07:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:07:35: 11000000 INFO @ Sat, 15 Jan 2022 21:07:36: 5000000 INFO @ Sat, 15 Jan 2022 21:07:36: 1000000 INFO @ Sat, 15 Jan 2022 21:07:41: 12000000 INFO @ Sat, 15 Jan 2022 21:07:43: 2000000 INFO @ Sat, 15 Jan 2022 21:07:43: 6000000 INFO @ Sat, 15 Jan 2022 21:07:45: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:07:45: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:07:45: #1 total tags in treatment: 5798889 INFO @ Sat, 15 Jan 2022 21:07:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:07:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:07:45: #1 tags after filtering in treatment: 3686354 INFO @ Sat, 15 Jan 2022 21:07:45: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 21:07:45: #1 finished! INFO @ Sat, 15 Jan 2022 21:07:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:07:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:07:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:07:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:07:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:07:49: 3000000 INFO @ Sat, 15 Jan 2022 21:07:51: 7000000 INFO @ Sat, 15 Jan 2022 21:07:55: 4000000 INFO @ Sat, 15 Jan 2022 21:07:58: 8000000 INFO @ Sat, 15 Jan 2022 21:08:02: 5000000 INFO @ Sat, 15 Jan 2022 21:08:05: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:08:08: 6000000 INFO @ Sat, 15 Jan 2022 21:08:12: 10000000 INFO @ Sat, 15 Jan 2022 21:08:14: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:08:19: 11000000 INFO @ Sat, 15 Jan 2022 21:08:21: 8000000 INFO @ Sat, 15 Jan 2022 21:08:27: 12000000 INFO @ Sat, 15 Jan 2022 21:08:27: 9000000 INFO @ Sat, 15 Jan 2022 21:08:31: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:08:31: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:08:31: #1 total tags in treatment: 5798889 INFO @ Sat, 15 Jan 2022 21:08:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:08:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:08:31: #1 tags after filtering in treatment: 3686354 INFO @ Sat, 15 Jan 2022 21:08:31: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 21:08:31: #1 finished! INFO @ Sat, 15 Jan 2022 21:08:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:08:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:08:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:08:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:08:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:08:33: 10000000 INFO @ Sat, 15 Jan 2022 21:08:39: 11000000 INFO @ Sat, 15 Jan 2022 21:08:44: 12000000 INFO @ Sat, 15 Jan 2022 21:08:48: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:08:48: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:08:48: #1 total tags in treatment: 5798889 INFO @ Sat, 15 Jan 2022 21:08:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:08:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:08:48: #1 tags after filtering in treatment: 3686354 INFO @ Sat, 15 Jan 2022 21:08:48: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 21:08:48: #1 finished! INFO @ Sat, 15 Jan 2022 21:08:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:08:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:08:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:08:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:08:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828585/SRX10828585.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling