Job ID = 14521429 SRX = SRX10828582 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 6490804 spots for SRR14480407/SRR14480407.sra Written 6490804 spots for SRR14480407/SRR14480407.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:41 6490804 reads; of these: 6490804 (100.00%) were paired; of these: 556271 (8.57%) aligned concordantly 0 times 5278976 (81.33%) aligned concordantly exactly 1 time 655557 (10.10%) aligned concordantly >1 times ---- 556271 pairs aligned concordantly 0 times; of these: 91928 (16.53%) aligned discordantly 1 time ---- 464343 pairs aligned 0 times concordantly or discordantly; of these: 928686 mates make up the pairs; of these: 768037 (82.70%) aligned 0 times 121286 (13.06%) aligned exactly 1 time 39363 (4.24%) aligned >1 times 94.08% overall alignment rate Time searching: 00:03:41 Overall time: 00:03:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 714260 / 5983991 = 0.1194 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:04:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:04:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:04:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:04:55: 1000000 INFO @ Sat, 15 Jan 2022 21:05:00: 2000000 INFO @ Sat, 15 Jan 2022 21:05:04: 3000000 INFO @ Sat, 15 Jan 2022 21:05:08: 4000000 INFO @ Sat, 15 Jan 2022 21:05:13: 5000000 INFO @ Sat, 15 Jan 2022 21:05:17: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:05:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:05:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:05:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:05:21: 7000000 INFO @ Sat, 15 Jan 2022 21:05:26: 8000000 INFO @ Sat, 15 Jan 2022 21:05:27: 1000000 INFO @ Sat, 15 Jan 2022 21:05:31: 9000000 INFO @ Sat, 15 Jan 2022 21:05:32: 2000000 INFO @ Sat, 15 Jan 2022 21:05:36: 10000000 INFO @ Sat, 15 Jan 2022 21:05:38: 3000000 INFO @ Sat, 15 Jan 2022 21:05:40: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:05:40: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:05:40: #1 total tags in treatment: 5223395 INFO @ Sat, 15 Jan 2022 21:05:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:05:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:05:40: #1 tags after filtering in treatment: 3410191 INFO @ Sat, 15 Jan 2022 21:05:40: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 21:05:40: #1 finished! INFO @ Sat, 15 Jan 2022 21:05:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:05:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:05:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:05:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:05:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:05:43: 4000000 INFO @ Sat, 15 Jan 2022 21:05:48: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:05:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:05:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:05:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:05:54: 6000000 INFO @ Sat, 15 Jan 2022 21:05:56: 1000000 INFO @ Sat, 15 Jan 2022 21:05:59: 7000000 INFO @ Sat, 15 Jan 2022 21:06:01: 2000000 INFO @ Sat, 15 Jan 2022 21:06:04: 8000000 INFO @ Sat, 15 Jan 2022 21:06:06: 3000000 INFO @ Sat, 15 Jan 2022 21:06:10: 9000000 INFO @ Sat, 15 Jan 2022 21:06:10: 4000000 INFO @ Sat, 15 Jan 2022 21:06:15: 5000000 INFO @ Sat, 15 Jan 2022 21:06:15: 10000000 INFO @ Sat, 15 Jan 2022 21:06:20: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:06:20: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:06:20: #1 total tags in treatment: 5223395 INFO @ Sat, 15 Jan 2022 21:06:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:06:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:06:20: #1 tags after filtering in treatment: 3410191 INFO @ Sat, 15 Jan 2022 21:06:20: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 21:06:20: #1 finished! INFO @ Sat, 15 Jan 2022 21:06:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:06:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:06:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:06:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:06:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.10_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 21:06:20: 6000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:06:25: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:06:29: 8000000 INFO @ Sat, 15 Jan 2022 21:06:34: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:06:38: 10000000 INFO @ Sat, 15 Jan 2022 21:06:42: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:06:42: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:06:42: #1 total tags in treatment: 5223395 INFO @ Sat, 15 Jan 2022 21:06:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:06:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:06:42: #1 tags after filtering in treatment: 3410191 INFO @ Sat, 15 Jan 2022 21:06:42: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 21:06:42: #1 finished! INFO @ Sat, 15 Jan 2022 21:06:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:06:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:06:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:06:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:06:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828582/SRX10828582.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling