Job ID = 14521428 SRX = SRX10828581 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3192182 spots for SRR14480406/SRR14480406.sra Written 3192182 spots for SRR14480406/SRR14480406.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:39 3192182 reads; of these: 3192182 (100.00%) were paired; of these: 496565 (15.56%) aligned concordantly 0 times 2395752 (75.05%) aligned concordantly exactly 1 time 299865 (9.39%) aligned concordantly >1 times ---- 496565 pairs aligned concordantly 0 times; of these: 74695 (15.04%) aligned discordantly 1 time ---- 421870 pairs aligned 0 times concordantly or discordantly; of these: 843740 mates make up the pairs; of these: 665617 (78.89%) aligned 0 times 139595 (16.54%) aligned exactly 1 time 38528 (4.57%) aligned >1 times 89.57% overall alignment rate Time searching: 00:01:39 Overall time: 00:01:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 279486 / 2728373 = 0.1024 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:01:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:01:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:01:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:01:14: 1000000 INFO @ Sat, 15 Jan 2022 21:01:19: 2000000 INFO @ Sat, 15 Jan 2022 21:01:23: 3000000 INFO @ Sat, 15 Jan 2022 21:01:28: 4000000 INFO @ Sat, 15 Jan 2022 21:01:32: 5000000 INFO @ Sat, 15 Jan 2022 21:01:33: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:01:33: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:01:33: #1 total tags in treatment: 2418422 INFO @ Sat, 15 Jan 2022 21:01:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:01:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:01:33: #1 tags after filtering in treatment: 1901812 INFO @ Sat, 15 Jan 2022 21:01:33: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 21:01:33: #1 finished! INFO @ Sat, 15 Jan 2022 21:01:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:01:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:01:33: #2 number of paired peaks: 50 WARNING @ Sat, 15 Jan 2022 21:01:33: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:01:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:01:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:01:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:01:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:01:44: 1000000 INFO @ Sat, 15 Jan 2022 21:01:49: 2000000 INFO @ Sat, 15 Jan 2022 21:01:54: 3000000 INFO @ Sat, 15 Jan 2022 21:01:58: 4000000 INFO @ Sat, 15 Jan 2022 21:02:03: 5000000 INFO @ Sat, 15 Jan 2022 21:02:03: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:02:03: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:02:03: #1 total tags in treatment: 2418422 INFO @ Sat, 15 Jan 2022 21:02:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:02:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:02:03: #1 tags after filtering in treatment: 1901812 INFO @ Sat, 15 Jan 2022 21:02:03: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 21:02:03: #1 finished! INFO @ Sat, 15 Jan 2022 21:02:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:02:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:02:03: #2 number of paired peaks: 50 WARNING @ Sat, 15 Jan 2022 21:02:03: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:02:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:02:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:02:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:02:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:02:14: 1000000 INFO @ Sat, 15 Jan 2022 21:02:19: 2000000 INFO @ Sat, 15 Jan 2022 21:02:23: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:02:28: 4000000 INFO @ Sat, 15 Jan 2022 21:02:32: 5000000 INFO @ Sat, 15 Jan 2022 21:02:33: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:02:33: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:02:33: #1 total tags in treatment: 2418422 INFO @ Sat, 15 Jan 2022 21:02:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:02:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:02:33: #1 tags after filtering in treatment: 1901812 INFO @ Sat, 15 Jan 2022 21:02:33: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 21:02:33: #1 finished! INFO @ Sat, 15 Jan 2022 21:02:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:02:33: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:02:33: #2 number of paired peaks: 50 WARNING @ Sat, 15 Jan 2022 21:02:33: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:02:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828581/SRX10828581.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling