Job ID = 14521407 SRX = SRX10828579 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8131210 spots for SRR14480404/SRR14480404.sra Written 8131210 spots for SRR14480404/SRR14480404.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:40 8131210 reads; of these: 8131210 (100.00%) were paired; of these: 1245090 (15.31%) aligned concordantly 0 times 6251262 (76.88%) aligned concordantly exactly 1 time 634858 (7.81%) aligned concordantly >1 times ---- 1245090 pairs aligned concordantly 0 times; of these: 253130 (20.33%) aligned discordantly 1 time ---- 991960 pairs aligned 0 times concordantly or discordantly; of these: 1983920 mates make up the pairs; of these: 1717954 (86.59%) aligned 0 times 195855 (9.87%) aligned exactly 1 time 70111 (3.53%) aligned >1 times 89.44% overall alignment rate Time searching: 00:06:40 Overall time: 00:06:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 926160 / 7032012 = 0.1317 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:08:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:08:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:08:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:08:46: 1000000 INFO @ Sat, 15 Jan 2022 21:08:55: 2000000 INFO @ Sat, 15 Jan 2022 21:09:03: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:09:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:09:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:09:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:09:12: 4000000 INFO @ Sat, 15 Jan 2022 21:09:17: 1000000 INFO @ Sat, 15 Jan 2022 21:09:21: 5000000 INFO @ Sat, 15 Jan 2022 21:09:26: 2000000 INFO @ Sat, 15 Jan 2022 21:09:31: 6000000 INFO @ Sat, 15 Jan 2022 21:09:35: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:09:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:09:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:09:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:09:40: 7000000 INFO @ Sat, 15 Jan 2022 21:09:44: 4000000 INFO @ Sat, 15 Jan 2022 21:09:48: 1000000 INFO @ Sat, 15 Jan 2022 21:09:50: 8000000 INFO @ Sat, 15 Jan 2022 21:09:53: 5000000 INFO @ Sat, 15 Jan 2022 21:09:57: 2000000 INFO @ Sat, 15 Jan 2022 21:10:01: 9000000 INFO @ Sat, 15 Jan 2022 21:10:03: 6000000 INFO @ Sat, 15 Jan 2022 21:10:07: 3000000 INFO @ Sat, 15 Jan 2022 21:10:11: 10000000 INFO @ Sat, 15 Jan 2022 21:10:12: 7000000 INFO @ Sat, 15 Jan 2022 21:10:17: 4000000 INFO @ Sat, 15 Jan 2022 21:10:20: 11000000 INFO @ Sat, 15 Jan 2022 21:10:21: 8000000 INFO @ Sat, 15 Jan 2022 21:10:27: 5000000 INFO @ Sat, 15 Jan 2022 21:10:30: 9000000 INFO @ Sat, 15 Jan 2022 21:10:30: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:10:36: 6000000 INFO @ Sat, 15 Jan 2022 21:10:37: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:10:37: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:10:37: #1 total tags in treatment: 5972383 INFO @ Sat, 15 Jan 2022 21:10:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:10:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:10:37: #1 tags after filtering in treatment: 3664289 INFO @ Sat, 15 Jan 2022 21:10:37: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 21:10:37: #1 finished! INFO @ Sat, 15 Jan 2022 21:10:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:10:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:10:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:10:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:10:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:10:40: 10000000 INFO @ Sat, 15 Jan 2022 21:10:46: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:10:49: 11000000 INFO @ Sat, 15 Jan 2022 21:10:56: 8000000 INFO @ Sat, 15 Jan 2022 21:10:58: 12000000 INFO @ Sat, 15 Jan 2022 21:11:04: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:11:04: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:11:04: #1 total tags in treatment: 5972383 INFO @ Sat, 15 Jan 2022 21:11:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:11:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:11:05: #1 tags after filtering in treatment: 3664289 INFO @ Sat, 15 Jan 2022 21:11:05: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 21:11:05: #1 finished! INFO @ Sat, 15 Jan 2022 21:11:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:11:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:11:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:11:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:11:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:11:06: 9000000 INFO @ Sat, 15 Jan 2022 21:11:15: 10000000 INFO @ Sat, 15 Jan 2022 21:11:24: 11000000 INFO @ Sat, 15 Jan 2022 21:11:34: 12000000 INFO @ Sat, 15 Jan 2022 21:11:40: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:11:40: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:11:40: #1 total tags in treatment: 5972383 INFO @ Sat, 15 Jan 2022 21:11:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:11:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:11:40: #1 tags after filtering in treatment: 3664289 INFO @ Sat, 15 Jan 2022 21:11:40: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 21:11:40: #1 finished! INFO @ Sat, 15 Jan 2022 21:11:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:11:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:11:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:11:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:11:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828579/SRX10828579.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling