Job ID = 14521406 SRX = SRX10828578 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 6994867 spots for SRR14480403/SRR14480403.sra Written 6994867 spots for SRR14480403/SRR14480403.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:30 6994867 reads; of these: 6994867 (100.00%) were paired; of these: 989233 (14.14%) aligned concordantly 0 times 5420610 (77.49%) aligned concordantly exactly 1 time 585024 (8.36%) aligned concordantly >1 times ---- 989233 pairs aligned concordantly 0 times; of these: 251491 (25.42%) aligned discordantly 1 time ---- 737742 pairs aligned 0 times concordantly or discordantly; of these: 1475484 mates make up the pairs; of these: 1224753 (83.01%) aligned 0 times 178055 (12.07%) aligned exactly 1 time 72676 (4.93%) aligned >1 times 91.25% overall alignment rate Time searching: 00:05:31 Overall time: 00:05:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 750655 / 6116289 = 0.1227 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:06:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:06:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:06:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:06:33: 1000000 INFO @ Sat, 15 Jan 2022 21:06:39: 2000000 INFO @ Sat, 15 Jan 2022 21:06:46: 3000000 INFO @ Sat, 15 Jan 2022 21:06:52: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:06:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:06:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:06:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:06:58: 5000000 INFO @ Sat, 15 Jan 2022 21:07:03: 6000000 INFO @ Sat, 15 Jan 2022 21:07:04: 1000000 INFO @ Sat, 15 Jan 2022 21:07:09: 7000000 INFO @ Sat, 15 Jan 2022 21:07:10: 2000000 INFO @ Sat, 15 Jan 2022 21:07:15: 8000000 INFO @ Sat, 15 Jan 2022 21:07:16: 3000000 INFO @ Sat, 15 Jan 2022 21:07:21: 9000000 INFO @ Sat, 15 Jan 2022 21:07:22: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:07:27: 10000000 INFO @ Sat, 15 Jan 2022 21:07:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:07:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:07:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:07:28: 5000000 INFO @ Sat, 15 Jan 2022 21:07:33: 11000000 INFO @ Sat, 15 Jan 2022 21:07:34: 1000000 INFO @ Sat, 15 Jan 2022 21:07:34: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:07:34: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:07:34: #1 total tags in treatment: 5263230 INFO @ Sat, 15 Jan 2022 21:07:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:07:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:07:34: 6000000 INFO @ Sat, 15 Jan 2022 21:07:35: #1 tags after filtering in treatment: 3351547 INFO @ Sat, 15 Jan 2022 21:07:35: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 21:07:35: #1 finished! INFO @ Sat, 15 Jan 2022 21:07:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:07:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:07:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:07:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:07:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:07:40: 2000000 INFO @ Sat, 15 Jan 2022 21:07:41: 7000000 INFO @ Sat, 15 Jan 2022 21:07:47: 3000000 INFO @ Sat, 15 Jan 2022 21:07:47: 8000000 INFO @ Sat, 15 Jan 2022 21:07:53: 4000000 INFO @ Sat, 15 Jan 2022 21:07:54: 9000000 INFO @ Sat, 15 Jan 2022 21:07:59: 5000000 INFO @ Sat, 15 Jan 2022 21:08:01: 10000000 INFO @ Sat, 15 Jan 2022 21:08:05: 6000000 INFO @ Sat, 15 Jan 2022 21:08:07: 11000000 INFO @ Sat, 15 Jan 2022 21:08:09: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:08:09: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:08:09: #1 total tags in treatment: 5263230 INFO @ Sat, 15 Jan 2022 21:08:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:08:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:08:09: #1 tags after filtering in treatment: 3351547 INFO @ Sat, 15 Jan 2022 21:08:09: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 21:08:09: #1 finished! INFO @ Sat, 15 Jan 2022 21:08:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:08:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:08:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:08:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:08:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:08:11: 7000000 INFO @ Sat, 15 Jan 2022 21:08:17: 8000000 INFO @ Sat, 15 Jan 2022 21:08:23: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:08:29: 10000000 INFO @ Sat, 15 Jan 2022 21:08:35: 11000000 INFO @ Sat, 15 Jan 2022 21:08:37: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:08:37: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:08:37: #1 total tags in treatment: 5263230 INFO @ Sat, 15 Jan 2022 21:08:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:08:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:08:37: #1 tags after filtering in treatment: 3351547 INFO @ Sat, 15 Jan 2022 21:08:37: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 21:08:37: #1 finished! INFO @ Sat, 15 Jan 2022 21:08:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:08:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:08:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:08:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:08:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10828578/SRX10828578.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling